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Amplification of HER-2/neu and topoisomerase IIalpha in primary and metastatic breast cancer.
Cancer Res. 2001 Jul 15; 61(14):5345-8.CR

Abstract

Amplification of the HER-2/neu oncogene and amplification of the topoisomerase IIalpha gene are important determinators of the response to chemotherapy in advanced breast cancer. Assays of these genes are usually carried out using primary tumor samples, because biopsies from metastatic lesions are not usually taken. We studied the concordance of Her-2/neu and topoisomerase IIalpha amplification in primary breast tumors and their metastases by immunostaining and DNA in situ hybridization. HER-2/neu amplification, present in 28% of the primary tumors (n = 46), was always associated with amplification in its metastasis. Conversely, no metastases with HER-2/neu amplification were seen without amplification in the primary tumor. Topoisomerase IIalpha gene copy status (amplification/deletion/unaltered) remained generally unchanged in HER-2/neu-positive tumors, but in three cases, the predominant cell population in metastatic tissue was present only as a subpopulation in the primary tumor. We conclude that amplification of HER-2/neu measured in primary tumor reflects the status of metastases. Minor discrepancies between primary and metastatic tumors in topoisomerase IIalpha gene copy status may reflect evolvement of the amplicon structure in successive cell divisions.

Authors+Show Affiliations

Laboratory of Cancer Genetics, Institute of Medical Technology, Tampere University and Tampere University Hospital, FIN-33014 Tampere, Finland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11454672

Citation

Tanner, M, et al. "Amplification of HER-2/neu and Topoisomerase IIalpha in Primary and Metastatic Breast Cancer." Cancer Research, vol. 61, no. 14, 2001, pp. 5345-8.
Tanner M, Järvinen P, Isola J. Amplification of HER-2/neu and topoisomerase IIalpha in primary and metastatic breast cancer. Cancer Res. 2001;61(14):5345-8.
Tanner, M., Järvinen, P., & Isola, J. (2001). Amplification of HER-2/neu and topoisomerase IIalpha in primary and metastatic breast cancer. Cancer Research, 61(14), 5345-8.
Tanner M, Järvinen P, Isola J. Amplification of HER-2/neu and Topoisomerase IIalpha in Primary and Metastatic Breast Cancer. Cancer Res. 2001 Jul 15;61(14):5345-8. PubMed PMID: 11454672.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amplification of HER-2/neu and topoisomerase IIalpha in primary and metastatic breast cancer. AU - Tanner,M, AU - Järvinen,P, AU - Isola,J, PY - 2001/7/17/pubmed PY - 2001/8/3/medline PY - 2001/7/17/entrez SP - 5345 EP - 8 JF - Cancer research JO - Cancer Res VL - 61 IS - 14 N2 - Amplification of the HER-2/neu oncogene and amplification of the topoisomerase IIalpha gene are important determinators of the response to chemotherapy in advanced breast cancer. Assays of these genes are usually carried out using primary tumor samples, because biopsies from metastatic lesions are not usually taken. We studied the concordance of Her-2/neu and topoisomerase IIalpha amplification in primary breast tumors and their metastases by immunostaining and DNA in situ hybridization. HER-2/neu amplification, present in 28% of the primary tumors (n = 46), was always associated with amplification in its metastasis. Conversely, no metastases with HER-2/neu amplification were seen without amplification in the primary tumor. Topoisomerase IIalpha gene copy status (amplification/deletion/unaltered) remained generally unchanged in HER-2/neu-positive tumors, but in three cases, the predominant cell population in metastatic tissue was present only as a subpopulation in the primary tumor. We conclude that amplification of HER-2/neu measured in primary tumor reflects the status of metastases. Minor discrepancies between primary and metastatic tumors in topoisomerase IIalpha gene copy status may reflect evolvement of the amplicon structure in successive cell divisions. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/11454672/Amplification_of_HER_2/neu_and_topoisomerase_IIalpha_in_primary_and_metastatic_breast_cancer_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=11454672 DB - PRIME DP - Unbound Medicine ER -