Tags

Type your tag names separated by a space and hit enter

Antitumor beta glucan from the cultured fruit body of Agaricus blazei.
Biol Pharm Bull. 2001 Jul; 24(7):820-8.BP

Abstract

Agaricus blazei is a medically important mushroom widely eaten and prescribed in Japan. Polysaccharide fractions were prepared from cultured A. blazei by repeated extraction with hot water (AgHWE), cold NaOH (AgCA), and then hot NaOH (AgHA). By chemical, enzymic, and NMR analyses, the primary structures of AgHWE, AgCA, and AgHA were mainly composed of 1,6-beta-glucan. Among these fractions, the NaOH extracts showed antitumor activity against the solid form of Sarcoma 180 in ICR mice. To demonstrate the active component in these fractions, several chemical and enzymic treatments were applied. These fractions were found to be i) neutral beta-glucan passing DEAE-Sephadex A-25, ii) resistant to periodate oxidation (I/B) and subsequent partial acid hydrolysis (I/B/H), iii) resistant to a 1,3-beta-glucanase, zymolyase, before I/B, but sensitive after I/B/H. In addition, after I/B/H treatment of the neutral fraction of AgCAE, a signal around 86 ppm attributable to 1,3-beta glucosidic linkage was detectable in the 13C-NMR spectrum. These facts strongly suggest that a highly branched 1,3-beta-glucan segment forms the active center of the antitumor activity.

Authors+Show Affiliations

Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy & Life Science, Hachioji, Japan. ohnonao@ps.toyaku.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11456124

Citation

Ohno, N, et al. "Antitumor Beta Glucan From the Cultured Fruit Body of Agaricus Blazei." Biological & Pharmaceutical Bulletin, vol. 24, no. 7, 2001, pp. 820-8.
Ohno N, Furukawa M, Miura NN, et al. Antitumor beta glucan from the cultured fruit body of Agaricus blazei. Biol Pharm Bull. 2001;24(7):820-8.
Ohno, N., Furukawa, M., Miura, N. N., Adachi, Y., Motoi, M., & Yadomae, T. (2001). Antitumor beta glucan from the cultured fruit body of Agaricus blazei. Biological & Pharmaceutical Bulletin, 24(7), 820-8.
Ohno N, et al. Antitumor Beta Glucan From the Cultured Fruit Body of Agaricus Blazei. Biol Pharm Bull. 2001;24(7):820-8. PubMed PMID: 11456124.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antitumor beta glucan from the cultured fruit body of Agaricus blazei. AU - Ohno,N, AU - Furukawa,M, AU - Miura,N N, AU - Adachi,Y, AU - Motoi,M, AU - Yadomae,T, PY - 2001/7/18/pubmed PY - 2002/1/5/medline PY - 2001/7/18/entrez SP - 820 EP - 8 JF - Biological & pharmaceutical bulletin JO - Biol Pharm Bull VL - 24 IS - 7 N2 - Agaricus blazei is a medically important mushroom widely eaten and prescribed in Japan. Polysaccharide fractions were prepared from cultured A. blazei by repeated extraction with hot water (AgHWE), cold NaOH (AgCA), and then hot NaOH (AgHA). By chemical, enzymic, and NMR analyses, the primary structures of AgHWE, AgCA, and AgHA were mainly composed of 1,6-beta-glucan. Among these fractions, the NaOH extracts showed antitumor activity against the solid form of Sarcoma 180 in ICR mice. To demonstrate the active component in these fractions, several chemical and enzymic treatments were applied. These fractions were found to be i) neutral beta-glucan passing DEAE-Sephadex A-25, ii) resistant to periodate oxidation (I/B) and subsequent partial acid hydrolysis (I/B/H), iii) resistant to a 1,3-beta-glucanase, zymolyase, before I/B, but sensitive after I/B/H. In addition, after I/B/H treatment of the neutral fraction of AgCAE, a signal around 86 ppm attributable to 1,3-beta glucosidic linkage was detectable in the 13C-NMR spectrum. These facts strongly suggest that a highly branched 1,3-beta-glucan segment forms the active center of the antitumor activity. SN - 0918-6158 UR - https://www.unboundmedicine.com/medline/citation/11456124/Antitumor_beta_glucan_from_the_cultured_fruit_body_of_Agaricus_blazei_ L2 - http://joi.jlc.jst.go.jp/JST.JSTAGE/bpb/24.820?from=PubMed DB - PRIME DP - Unbound Medicine ER -