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Messenger RNAs are recruited for nuclear export during transcription.
Genes Dev. 2001 Jul 15; 15(14):1771-82.GD

Abstract

Following transcription and processing, eukaryotic mRNAs are exported from the nucleus to the cytoplasm for translation. Here we present evidence that mRNAs are targeted for nuclear export cotranscriptionally. Combined mutations in the Saccharomyces cerevisiae hnRNP Npl3 and TATA-binding protein (TBP) block mRNA export, implying that cotranscriptional recruitment of Npl3 is required for efficient export of mRNA. Furthermore, Npl3 can be found in a complex with RNA Pol II, indicating that Npl3 associates with the transcription machinery. Finally, Npl3 is recruited to genes in a transcription dependent manner as determined by chromatin immunoprecipitation. Another mRNA export factor, Yra1, also associates with chromatin cotranscriptionally but appears to be recruited at a later step. Taken together, our results suggest that export factors are recruited to the sites of transcription to promote efficient mRNA export.

Authors+Show Affiliations

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11459827

Citation

Lei, E P., et al. "Messenger RNAs Are Recruited for Nuclear Export During Transcription." Genes & Development, vol. 15, no. 14, 2001, pp. 1771-82.
Lei EP, Krebber H, Silver PA. Messenger RNAs are recruited for nuclear export during transcription. Genes Dev. 2001;15(14):1771-82.
Lei, E. P., Krebber, H., & Silver, P. A. (2001). Messenger RNAs are recruited for nuclear export during transcription. Genes & Development, 15(14), 1771-82.
Lei EP, Krebber H, Silver PA. Messenger RNAs Are Recruited for Nuclear Export During Transcription. Genes Dev. 2001 Jul 15;15(14):1771-82. PubMed PMID: 11459827.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Messenger RNAs are recruited for nuclear export during transcription. AU - Lei,E P, AU - Krebber,H, AU - Silver,P A, PY - 2001/7/19/pubmed PY - 2001/8/17/medline PY - 2001/7/19/entrez SP - 1771 EP - 82 JF - Genes & development JO - Genes Dev VL - 15 IS - 14 N2 - Following transcription and processing, eukaryotic mRNAs are exported from the nucleus to the cytoplasm for translation. Here we present evidence that mRNAs are targeted for nuclear export cotranscriptionally. Combined mutations in the Saccharomyces cerevisiae hnRNP Npl3 and TATA-binding protein (TBP) block mRNA export, implying that cotranscriptional recruitment of Npl3 is required for efficient export of mRNA. Furthermore, Npl3 can be found in a complex with RNA Pol II, indicating that Npl3 associates with the transcription machinery. Finally, Npl3 is recruited to genes in a transcription dependent manner as determined by chromatin immunoprecipitation. Another mRNA export factor, Yra1, also associates with chromatin cotranscriptionally but appears to be recruited at a later step. Taken together, our results suggest that export factors are recruited to the sites of transcription to promote efficient mRNA export. SN - 0890-9369 UR - https://www.unboundmedicine.com/medline/citation/11459827/Messenger_RNAs_are_recruited_for_nuclear_export_during_transcription_ DB - PRIME DP - Unbound Medicine ER -