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DNA replication meets genetic exchange: chromosomal damage and its repair by homologous recombination.
Proc Natl Acad Sci U S A. 2001 Jul 17; 98(15):8461-8.PN

Abstract

Proceedings of the National Academy of Sciences Colloquium on the roles of homologous recombination in DNA replication are summarized. Current findings in experimental systems ranging from bacteriophages to mammalian cell lines substantiate the idea that homologous recombination is a system supporting DNA replication when either the template DNA is damaged or the replication machinery malfunctions. There are several lines of supporting evidence: (i) DNA replication aggravates preexisting DNA damage, which then blocks subsequent replication; (ii) replication forks abandoned by malfunctioning replisomes become prone to breakage; (iii) mutants with malfunctioning replisomes or with elevated levels of DNA damage depend on homologous recombination; and (iv) homologous recombination primes DNA replication in vivo and can restore replication fork structures in vitro. The mechanisms of recombinational repair in bacteriophage T4, Escherichia coli, and Saccharomyces cerevisiae are compared. In vitro properties of the eukaryotic recombinases suggest a bigger role for single-strand annealing in the eukaryotic recombinational repair.

Authors+Show Affiliations

Department of Microbiology, University of Illinois, Urbana-Champaign, B103, Chemical and Life Sciences Laboratory, 601 South Goodwin Avenue, Urbana, IL 61801-3709, USA. kuzminov@life.uiuc.edu

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Review

Language

eng

PubMed ID

11459990

Citation

Kuzminov, A. "DNA Replication Meets Genetic Exchange: Chromosomal Damage and Its Repair By Homologous Recombination." Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 15, 2001, pp. 8461-8.
Kuzminov A. DNA replication meets genetic exchange: chromosomal damage and its repair by homologous recombination. Proc Natl Acad Sci U S A. 2001;98(15):8461-8.
Kuzminov, A. (2001). DNA replication meets genetic exchange: chromosomal damage and its repair by homologous recombination. Proceedings of the National Academy of Sciences of the United States of America, 98(15), 8461-8.
Kuzminov A. DNA Replication Meets Genetic Exchange: Chromosomal Damage and Its Repair By Homologous Recombination. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8461-8. PubMed PMID: 11459990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA replication meets genetic exchange: chromosomal damage and its repair by homologous recombination. A1 - Kuzminov,A, PY - 2001/7/19/pubmed PY - 2001/8/31/medline PY - 2001/7/19/entrez SP - 8461 EP - 8 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 98 IS - 15 N2 - Proceedings of the National Academy of Sciences Colloquium on the roles of homologous recombination in DNA replication are summarized. Current findings in experimental systems ranging from bacteriophages to mammalian cell lines substantiate the idea that homologous recombination is a system supporting DNA replication when either the template DNA is damaged or the replication machinery malfunctions. There are several lines of supporting evidence: (i) DNA replication aggravates preexisting DNA damage, which then blocks subsequent replication; (ii) replication forks abandoned by malfunctioning replisomes become prone to breakage; (iii) mutants with malfunctioning replisomes or with elevated levels of DNA damage depend on homologous recombination; and (iv) homologous recombination primes DNA replication in vivo and can restore replication fork structures in vitro. The mechanisms of recombinational repair in bacteriophage T4, Escherichia coli, and Saccharomyces cerevisiae are compared. In vitro properties of the eukaryotic recombinases suggest a bigger role for single-strand annealing in the eukaryotic recombinational repair. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/11459990/DNA_replication_meets_genetic_exchange:_chromosomal_damage_and_its_repair_by_homologous_recombination_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=11459990 DB - PRIME DP - Unbound Medicine ER -