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Effects of copper and cadmium exposure on functional responses of hemocytes in the clam, Tapes philippinarum.
Arch Environ Contam Toxicol. 2001 Aug; 41(2):163-70.AE

Abstract

The effects of Cu and Cd on functional responses of hemocytes in Tapes philippinarum, a Veneridae clam widespread in the lagoon of Venice, were evaluated. Bivalves were exposed for 7 days to various concentrations of CuCl(2) x 2H(2)O (0, 10, 60, and 110 microg/L) and CdCl(2) x H(2)O (0, 150, 300, and 450 microg/L). Hemocytes were collected from exposed clams, and the effects of Cu and Cd on phagocytosis, Neutral Red retention capacity, superoxide dismutase, and cytochrome oxidase activities were investigated. Hemocytes from animals exposed to Cu showed a significant decrease in phagocytic activity, whereas no inhibition was observed in cells from Cd-exposed animals. Diffusion of Neutral Red into the cytosol, an index of lysosomal membrane alteration, was enhanced by high concentrations of both metals. Different results in biochemical parameters were observed. Exposure to 60 and 110 microg Cu/L caused a significant reduction in hemocyte superoxide dismutase activity, whereas no changes resulted after Cd exposure. Increased cytochrome oxidase activity was observed in hemocytes from mollusks exposed to 60 microg Cu/L and 300 microg Cd/L; no significant differences were found in cells from bivalves exposed to 110 microg Cu/L and 450 microg Cd/L. These results suggest a relationship between heavy metal exposure and alterations in functional responses of hemocytes in T. philippinarum and indicate that the type of observed effects vary with the nature and concentration of heavy metals. Moreover, the data obtained in the analyzed clam support the possibility of using it as sentinel organism in biomonitoring studies, even if used biomarkers will be further evaluated in field conditions.

Authors+Show Affiliations

Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35121 Padova, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11462140

Citation

Matozzo, V, et al. "Effects of Copper and Cadmium Exposure On Functional Responses of Hemocytes in the Clam, Tapes Philippinarum." Archives of Environmental Contamination and Toxicology, vol. 41, no. 2, 2001, pp. 163-70.
Matozzo V, Ballarin L, Pampanin DM, et al. Effects of copper and cadmium exposure on functional responses of hemocytes in the clam, Tapes philippinarum. Arch Environ Contam Toxicol. 2001;41(2):163-70.
Matozzo, V., Ballarin, L., Pampanin, D. M., & Marin, M. G. (2001). Effects of copper and cadmium exposure on functional responses of hemocytes in the clam, Tapes philippinarum. Archives of Environmental Contamination and Toxicology, 41(2), 163-70.
Matozzo V, et al. Effects of Copper and Cadmium Exposure On Functional Responses of Hemocytes in the Clam, Tapes Philippinarum. Arch Environ Contam Toxicol. 2001;41(2):163-70. PubMed PMID: 11462140.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of copper and cadmium exposure on functional responses of hemocytes in the clam, Tapes philippinarum. AU - Matozzo,V, AU - Ballarin,L, AU - Pampanin,D M, AU - Marin,M G, PY - 2000/11/06/received PY - 2001/03/06/accepted PY - 2001/7/20/pubmed PY - 2001/9/8/medline PY - 2001/7/20/entrez SP - 163 EP - 70 JF - Archives of environmental contamination and toxicology JO - Arch Environ Contam Toxicol VL - 41 IS - 2 N2 - The effects of Cu and Cd on functional responses of hemocytes in Tapes philippinarum, a Veneridae clam widespread in the lagoon of Venice, were evaluated. Bivalves were exposed for 7 days to various concentrations of CuCl(2) x 2H(2)O (0, 10, 60, and 110 microg/L) and CdCl(2) x H(2)O (0, 150, 300, and 450 microg/L). Hemocytes were collected from exposed clams, and the effects of Cu and Cd on phagocytosis, Neutral Red retention capacity, superoxide dismutase, and cytochrome oxidase activities were investigated. Hemocytes from animals exposed to Cu showed a significant decrease in phagocytic activity, whereas no inhibition was observed in cells from Cd-exposed animals. Diffusion of Neutral Red into the cytosol, an index of lysosomal membrane alteration, was enhanced by high concentrations of both metals. Different results in biochemical parameters were observed. Exposure to 60 and 110 microg Cu/L caused a significant reduction in hemocyte superoxide dismutase activity, whereas no changes resulted after Cd exposure. Increased cytochrome oxidase activity was observed in hemocytes from mollusks exposed to 60 microg Cu/L and 300 microg Cd/L; no significant differences were found in cells from bivalves exposed to 110 microg Cu/L and 450 microg Cd/L. These results suggest a relationship between heavy metal exposure and alterations in functional responses of hemocytes in T. philippinarum and indicate that the type of observed effects vary with the nature and concentration of heavy metals. Moreover, the data obtained in the analyzed clam support the possibility of using it as sentinel organism in biomonitoring studies, even if used biomarkers will be further evaluated in field conditions. SN - 0090-4341 UR - https://www.unboundmedicine.com/medline/citation/11462140/Effects_of_copper_and_cadmium_exposure_on_functional_responses_of_hemocytes_in_the_clam_Tapes_philippinarum_ L2 - https://dx.doi.org/10.1007/s002440010234 DB - PRIME DP - Unbound Medicine ER -