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Detailed molecular analysis of 1p36 in neuroblastoma.
Med Pediatr Oncol. 2001 Jan; 36(1):37-41.MP

Abstract

BACKGROUND

Several lines of evidence es tablish that chromosome band 1p36 is frequently deleted in neuroblastoma primary tumors and cell lines, suggesting that a tumor suppressor gene within this region is involved in the development of this tumor.

PROCEDURE

We analyzed the status of 1p36 in primary neuroblastomas and cell lines to define the region of consistent rearrangement.

RESULTS

Loss of heterozygosity (LOH) studies of primary neuro blastomas identified allelic loss in 135 of 503 tumors (27%), with the smallest region of overlap (SRO) defined distal to D15214 (1p36.3). No homozygous deletions were detected at 120 loci mapping to 1p36.1-p36.3 in a panel of 46 neuroblastoma cell lines. A recently identified patient with neuroblastoma was found to have a constitutional deletion within 1p36.2-p36.3, and this deletion, when combined with the LOH results, defined a smaller SRO of one megabase within 1p36.3. We constructed a comprehensive integrated map of chromosome 1 containing 11,000 markers and large-insert clones, a high-resolution radiation hybrid (RH) map of 1p36, and a P1-artificial chromosome (PAC) contig spanning the SRO, to further characterize the region of interest. Over 768 kb (75%) of the SRO has been sequenced to completion. Further analysis of distal 1p identified 113 transcripts localizing to 1p36, 21 of which were mapped within the SRO.

CONCLUSION

This analysis will identify suitable positional candidate transcripts for mutational screening and subsequent identification of the 1p36.3 neuroblastoma suppressor gene.

Authors+Show Affiliations

Division of Oncology, Children's Hospital of Philadelphia, Pennsylvania 19104-4318, USA. white@genome.chop.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Multicenter Study
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11464901

Citation

White, P S., et al. "Detailed Molecular Analysis of 1p36 in Neuroblastoma." Medical and Pediatric Oncology, vol. 36, no. 1, 2001, pp. 37-41.
White PS, Thompson PM, Seifried BA, et al. Detailed molecular analysis of 1p36 in neuroblastoma. Med Pediatr Oncol. 2001;36(1):37-41.
White, P. S., Thompson, P. M., Seifried, B. A., Sulman, E. P., Jensen, S. J., Guo, C., Maris, J. M., Hogarty, M. D., Allen, C., Biegel, J. A., Matise, T. C., Gregory, S. G., Reynolds, C. P., & Brodeur, G. M. (2001). Detailed molecular analysis of 1p36 in neuroblastoma. Medical and Pediatric Oncology, 36(1), 37-41.
White PS, et al. Detailed Molecular Analysis of 1p36 in Neuroblastoma. Med Pediatr Oncol. 2001;36(1):37-41. PubMed PMID: 11464901.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detailed molecular analysis of 1p36 in neuroblastoma. AU - White,P S, AU - Thompson,P M, AU - Seifried,B A, AU - Sulman,E P, AU - Jensen,S J, AU - Guo,C, AU - Maris,J M, AU - Hogarty,M D, AU - Allen,C, AU - Biegel,J A, AU - Matise,T C, AU - Gregory,S G, AU - Reynolds,C P, AU - Brodeur,G M, PY - 2001/7/24/pubmed PY - 2001/8/17/medline PY - 2001/7/24/entrez SP - 37 EP - 41 JF - Medical and pediatric oncology JO - Med Pediatr Oncol VL - 36 IS - 1 N2 - BACKGROUND: Several lines of evidence es tablish that chromosome band 1p36 is frequently deleted in neuroblastoma primary tumors and cell lines, suggesting that a tumor suppressor gene within this region is involved in the development of this tumor. PROCEDURE: We analyzed the status of 1p36 in primary neuroblastomas and cell lines to define the region of consistent rearrangement. RESULTS: Loss of heterozygosity (LOH) studies of primary neuro blastomas identified allelic loss in 135 of 503 tumors (27%), with the smallest region of overlap (SRO) defined distal to D15214 (1p36.3). No homozygous deletions were detected at 120 loci mapping to 1p36.1-p36.3 in a panel of 46 neuroblastoma cell lines. A recently identified patient with neuroblastoma was found to have a constitutional deletion within 1p36.2-p36.3, and this deletion, when combined with the LOH results, defined a smaller SRO of one megabase within 1p36.3. We constructed a comprehensive integrated map of chromosome 1 containing 11,000 markers and large-insert clones, a high-resolution radiation hybrid (RH) map of 1p36, and a P1-artificial chromosome (PAC) contig spanning the SRO, to further characterize the region of interest. Over 768 kb (75%) of the SRO has been sequenced to completion. Further analysis of distal 1p identified 113 transcripts localizing to 1p36, 21 of which were mapped within the SRO. CONCLUSION: This analysis will identify suitable positional candidate transcripts for mutational screening and subsequent identification of the 1p36.3 neuroblastoma suppressor gene. SN - 0098-1532 UR - https://www.unboundmedicine.com/medline/citation/11464901/Detailed_molecular_analysis_of_1p36_in_neuroblastoma_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0098-1532&date=2001&volume=36&issue=1&spage=37 DB - PRIME DP - Unbound Medicine ER -