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Fine mapping of a tumour suppressor candidate gene region in 1p36.2-3, commonly deleted in neuroblastomas and germ cell tumours.
Med Pediatr Oncol. 2001 Jan; 36(1):61-6.MP

Abstract

BACKGROUND

A common genetic feature of neuroblastomas, which is also an important prognostic factor, is deletion of chromosome region 1p. The deletion of 1p often involves a deletion of varying size, with a consensus region within the most distal bands 1p36.2-3. The neuroblastoma SRO (shortest region of overlap of (deletions) presented earlier by our group was defined distally by the cluster of loci D1S80/ D1Z2/CDC2L1 and proximally by loci D1S244, i.e., approximately 25 cM. The 1p deletions are, however, not restricted to neuroblastoma tumours. In fact, a large spectrum of tumour types display deletions to varying degrees of 1p.

PROCEDURE

We have exploited the possibility of using deletions of other tumour types, preferentially that of germ cell tumours, and combining the deletions with that of the neuroblastoma SRO. Also in germ cell tumours, distal 1p-deletions have been shown to have prognostic significance.

RESULTS

We found in our germ cell tumours a SRO ranging from D1S508 to D1S200. Interestingly, this region only partially overlapped (approximately 5 cm) with our neuroblastoma SRO in region D1S508 to D1S244. We have thus focused on analysing this smaller region in the search for genes involved in the genesis of different cancers. We have performed radiation hybrid mapping of a large number of markers, STSs, ESTs, and others known to reside in 1p. We have also initiated the development of a BAC contig of the region. FISH, and fibre-FISH mapping of BACs were also performed.

CONCLUSIONS

The data presented here constitute an ongoing work with the aim of identifying and cloning gene(s) important for development of germ cell tumours, neuroblastomas, and possibly other tumours.

Authors+Show Affiliations

Department of Clinical Genetics, Sahlgrenska University Hospital/East, Gothenburg, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11464908

Citation

Ejeskär, K, et al. "Fine Mapping of a Tumour Suppressor Candidate Gene Region in 1p36.2-3, Commonly Deleted in Neuroblastomas and Germ Cell Tumours." Medical and Pediatric Oncology, vol. 36, no. 1, 2001, pp. 61-6.
Ejeskär K, Sjöberg RM, Abel F, et al. Fine mapping of a tumour suppressor candidate gene region in 1p36.2-3, commonly deleted in neuroblastomas and germ cell tumours. Med Pediatr Oncol. 2001;36(1):61-6.
Ejeskär, K., Sjöberg, R. M., Abel, F., Kogner, P., Ambros, P. F., & Martinsson, T. (2001). Fine mapping of a tumour suppressor candidate gene region in 1p36.2-3, commonly deleted in neuroblastomas and germ cell tumours. Medical and Pediatric Oncology, 36(1), 61-6.
Ejeskär K, et al. Fine Mapping of a Tumour Suppressor Candidate Gene Region in 1p36.2-3, Commonly Deleted in Neuroblastomas and Germ Cell Tumours. Med Pediatr Oncol. 2001;36(1):61-6. PubMed PMID: 11464908.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fine mapping of a tumour suppressor candidate gene region in 1p36.2-3, commonly deleted in neuroblastomas and germ cell tumours. AU - Ejeskär,K, AU - Sjöberg,R M, AU - Abel,F, AU - Kogner,P, AU - Ambros,P F, AU - Martinsson,T, PY - 2001/7/24/pubmed PY - 2001/8/17/medline PY - 2001/7/24/entrez SP - 61 EP - 6 JF - Medical and pediatric oncology JO - Med Pediatr Oncol VL - 36 IS - 1 N2 - BACKGROUND: A common genetic feature of neuroblastomas, which is also an important prognostic factor, is deletion of chromosome region 1p. The deletion of 1p often involves a deletion of varying size, with a consensus region within the most distal bands 1p36.2-3. The neuroblastoma SRO (shortest region of overlap of (deletions) presented earlier by our group was defined distally by the cluster of loci D1S80/ D1Z2/CDC2L1 and proximally by loci D1S244, i.e., approximately 25 cM. The 1p deletions are, however, not restricted to neuroblastoma tumours. In fact, a large spectrum of tumour types display deletions to varying degrees of 1p. PROCEDURE: We have exploited the possibility of using deletions of other tumour types, preferentially that of germ cell tumours, and combining the deletions with that of the neuroblastoma SRO. Also in germ cell tumours, distal 1p-deletions have been shown to have prognostic significance. RESULTS: We found in our germ cell tumours a SRO ranging from D1S508 to D1S200. Interestingly, this region only partially overlapped (approximately 5 cm) with our neuroblastoma SRO in region D1S508 to D1S244. We have thus focused on analysing this smaller region in the search for genes involved in the genesis of different cancers. We have performed radiation hybrid mapping of a large number of markers, STSs, ESTs, and others known to reside in 1p. We have also initiated the development of a BAC contig of the region. FISH, and fibre-FISH mapping of BACs were also performed. CONCLUSIONS: The data presented here constitute an ongoing work with the aim of identifying and cloning gene(s) important for development of germ cell tumours, neuroblastomas, and possibly other tumours. SN - 0098-1532 UR - https://www.unboundmedicine.com/medline/citation/11464908/Fine_mapping_of_a_tumour_suppressor_candidate_gene_region_in_1p36_2_3_commonly_deleted_in_neuroblastomas_and_germ_cell_tumours_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0098-1532&date=2001&volume=36&issue=1&spage=61 DB - PRIME DP - Unbound Medicine ER -