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State of HBV DNA in HBsAg-negative, anti-HCV-positive hepatocellular carcinoma: existence of HBV DNA possibly as nonintegrated form with analysis by Alu-HBV DNA PCR and conventional HBV PCR.
J Med Virol 2001; 64(4):410-8JM

Abstract

The role of hepatitis B virus (HBV) in carcinogenesis of hepatitis B surface antigen (HBsAg)-negative, anti-hepatitis C virus (anti-HCV)-positive hepatocellular carcinoma (HCC) remains unknown. To investigate the state of HBV DNA in such HCC, HBV DNA was examined by polymerase chain reaction (PCR) between HBV DNA and human Alu sequence (HBV-Alu PCR), which could detect integrated form of HBV DNA only, and by conventional HBV PCR, which could detect both integrated and episomal forms of HBV DNA. In all the 17 HBsAg-positive HCC, HBV DNA was detected by both HBV-Alu PCR method and conventional HBV PCR method. By contrast, in HBsAg-negative, anti-HCV-positive cases, HBV DNA was detected in 10 of 21 (47.6%) by conventional HBV PCR and in none of 21 (0%) by HBV-Alu PCR method. Thus, integrated form of HBV DNA was not found in most HbsAg-negative, anti-HCV-positive HCC in the current study. The role of episomal form of HBV DNA requires further investigation of its involvement in the process of the development of HBsAg-negative, anti-HCV-positive HCC.

Authors+Show Affiliations

First Department of Medicine, Chiba University School of Medicine, Chiba, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

11468724

Citation

Kawai, S, et al. "State of HBV DNA in HBsAg-negative, anti-HCV-positive Hepatocellular Carcinoma: Existence of HBV DNA Possibly as Nonintegrated Form With Analysis By Alu-HBV DNA PCR and Conventional HBV PCR." Journal of Medical Virology, vol. 64, no. 4, 2001, pp. 410-8.
Kawai S, Yokosuka O, Imazeki F, et al. State of HBV DNA in HBsAg-negative, anti-HCV-positive hepatocellular carcinoma: existence of HBV DNA possibly as nonintegrated form with analysis by Alu-HBV DNA PCR and conventional HBV PCR. J Med Virol. 2001;64(4):410-8.
Kawai, S., Yokosuka, O., Imazeki, F., Maru, Y., & Saisho, H. (2001). State of HBV DNA in HBsAg-negative, anti-HCV-positive hepatocellular carcinoma: existence of HBV DNA possibly as nonintegrated form with analysis by Alu-HBV DNA PCR and conventional HBV PCR. Journal of Medical Virology, 64(4), pp. 410-8.
Kawai S, et al. State of HBV DNA in HBsAg-negative, anti-HCV-positive Hepatocellular Carcinoma: Existence of HBV DNA Possibly as Nonintegrated Form With Analysis By Alu-HBV DNA PCR and Conventional HBV PCR. J Med Virol. 2001;64(4):410-8. PubMed PMID: 11468724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - State of HBV DNA in HBsAg-negative, anti-HCV-positive hepatocellular carcinoma: existence of HBV DNA possibly as nonintegrated form with analysis by Alu-HBV DNA PCR and conventional HBV PCR. AU - Kawai,S, AU - Yokosuka,O, AU - Imazeki,F, AU - Maru,Y, AU - Saisho,H, PY - 2001/7/27/pubmed PY - 2001/8/31/medline PY - 2001/7/27/entrez SP - 410 EP - 8 JF - Journal of medical virology JO - J. Med. Virol. VL - 64 IS - 4 N2 - The role of hepatitis B virus (HBV) in carcinogenesis of hepatitis B surface antigen (HBsAg)-negative, anti-hepatitis C virus (anti-HCV)-positive hepatocellular carcinoma (HCC) remains unknown. To investigate the state of HBV DNA in such HCC, HBV DNA was examined by polymerase chain reaction (PCR) between HBV DNA and human Alu sequence (HBV-Alu PCR), which could detect integrated form of HBV DNA only, and by conventional HBV PCR, which could detect both integrated and episomal forms of HBV DNA. In all the 17 HBsAg-positive HCC, HBV DNA was detected by both HBV-Alu PCR method and conventional HBV PCR method. By contrast, in HBsAg-negative, anti-HCV-positive cases, HBV DNA was detected in 10 of 21 (47.6%) by conventional HBV PCR and in none of 21 (0%) by HBV-Alu PCR method. Thus, integrated form of HBV DNA was not found in most HbsAg-negative, anti-HCV-positive HCC in the current study. The role of episomal form of HBV DNA requires further investigation of its involvement in the process of the development of HBsAg-negative, anti-HCV-positive HCC. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/11468724/State_of_HBV_DNA_in_HBsAg_negative_anti_HCV_positive_hepatocellular_carcinoma:_existence_of_HBV_DNA_possibly_as_nonintegrated_form_with_analysis_by_Alu_HBV_DNA_PCR_and_conventional_HBV_PCR_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0146-6615&date=2001&volume=64&issue=4&spage=410 DB - PRIME DP - Unbound Medicine ER -