TY - JOUR T1 - Isolation and characterization of a gene specific to lager brewing yeast that encodes a branched-chain amino acid permease. AU - Kodama,Y, AU - Omura,F, AU - Ashikari,T, PY - 2001/7/27/pubmed PY - 2001/10/26/medline PY - 2001/7/27/entrez SP - 3455 EP - 62 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 67 IS - 8 N2 - We found two types of branched-chain amino acid permease gene (BAP2) in the lager brewing yeast Saccharomyces pastorianus BH-225 and cloned one type of BAP2 gene (Lg-BAP2), which is identical to that of Saccharomyces bayanus (by-BAP2-1). The other BAP2 gene of the lager brewing yeast (cer-BAP2) is very similar to the Saccharomyces cerevisiae BAP2 gene. This result substantiates the notion that lager brewing yeast is a hybrid of S. cerevisiae and S. bayanus. The amino acid sequence homology between S. cerevisiae Bap2p and Lg-Bap2p was 88%. The transcription of Lg-BAP2 was not induced by the addition of leucine to the growth medium, while that of cer-BAP2 was induced. The transcription of Lg-BAP2 was repressed by the presence of ethanol and weak organic acid, while that of cer-BAP2 was not affected by these compounds. Furthermore, Northern analysis during beer fermentation revealed that the transcription of Lg-BAP2 was repressed at the beginning of the fermentation, while cer-BAP2 was highly expressed throughout the fermentation. These results suggest that the transcription of Lg-BAP2 is regulated differently from that of cer-BAP2 in lager brewing yeasts. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/11472919/Isolation_and_characterization_of_a_gene_specific_to_lager_brewing_yeast_that_encodes_a_branched_chain_amino_acid_permease_ L2 - https://journals.asm.org/doi/10.1128/AEM.67.8.3455-3462.2001?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -