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Malaria rapid diagnostic devices: performance characteristics of the ParaSight F device determined in a multisite field study.
J Clin Microbiol. 2001 Aug; 39(8):2884-90.JC

Abstract

Microscopic detection of parasites has been the reference standard for malaria diagnosis for decades. However, difficulty in maintaining required technical skills and infrastructure has spurred the development of several nonmicroscopic malaria rapid diagnostic devices based on the detection of malaria parasite antigen in whole blood. The ParaSight F test is one such device. It detects the presence of Plasmodium falciparum-specific histidine-rich protein 2 by using an antigen-capture immunochromatographic strip format. The present study was conducted at outpatient malaria clinics in Iquitos, Peru, and Maesod, Thailand. Duplicate, blinded, expert microscopy was employed as the reference standard for evaluating device performance. Of 2,988 eligible patients, microscopy showed that 547 (18%) had P. falciparum, 658 (22%) had P. vivax, 2 (0.07%) had P. malariae, and 1,750 (59%) were negative for Plasmodium. Mixed infections (P. falciparum and P. vivax) were identified in 31 patients (1%). The overall sensitivity of ParaSight F for P. falciparum was 95%. When stratified by magnitude of parasitemia (no. of asexual parasites per microliter of whole blood), sensitivities were 83% (>0 to 500 parasites/microl), 87% (501 to 1,000/microl), 98% (1,001 to 5,000/microl), and 98% (>5,000/microl). Device specificity was 86%.

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Authors+Show Affiliations

Forney JR
Walter Reed Army Institute of Research, Washington, D.C., USA. mj9173@usma.edu
Magill AJ
No affiliation info available
Wongsrichanalai C
No affiliation info available
Sirichaisinthop J
No affiliation info available
Bautista CT
No affiliation info available
Heppner DG
No affiliation info available
Miller RS
No affiliation info available
Ockenhouse CF
No affiliation info available
Gubanov A
No affiliation info available
Shafer R
No affiliation info available
DeWitt CC
No affiliation info available
Quino-Ascurra HA
No affiliation info available
Kester KE
No affiliation info available
Kain KC
No affiliation info available
Walsh DS
No affiliation info available
Ballou WR
No affiliation info available
Gasser RA
No affiliation info available

MeSH

AnimalsAntigens, ProtozoanHumansImmunoassayMalaria, FalciparumParasitemiaPlasmodium falciparumProteinsReagent Kits, DiagnosticReagent StripsSensitivity and SpecificityTime Factors

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

11474008

Citation

Forney, J R., et al. "Malaria Rapid Diagnostic Devices: Performance Characteristics of the ParaSight F Device Determined in a Multisite Field Study." Journal of Clinical Microbiology, vol. 39, no. 8, 2001, pp. 2884-90.
Forney JR, Magill AJ, Wongsrichanalai C, et al. Malaria rapid diagnostic devices: performance characteristics of the ParaSight F device determined in a multisite field study. J Clin Microbiol. 2001;39(8):2884-90.
Forney, J. R., Magill, A. J., Wongsrichanalai, C., Sirichaisinthop, J., Bautista, C. T., Heppner, D. G., Miller, R. S., Ockenhouse, C. F., Gubanov, A., Shafer, R., DeWitt, C. C., Quino-Ascurra, H. A., Kester, K. E., Kain, K. C., Walsh, D. S., Ballou, W. R., & Gasser, R. A. (2001). Malaria rapid diagnostic devices: performance characteristics of the ParaSight F device determined in a multisite field study. Journal of Clinical Microbiology, 39(8), 2884-90.
Forney JR, et al. Malaria Rapid Diagnostic Devices: Performance Characteristics of the ParaSight F Device Determined in a Multisite Field Study. J Clin Microbiol. 2001;39(8):2884-90. PubMed PMID: 11474008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Malaria rapid diagnostic devices: performance characteristics of the ParaSight F device determined in a multisite field study. AU - Forney,J R, AU - Magill,A J, AU - Wongsrichanalai,C, AU - Sirichaisinthop,J, AU - Bautista,C T, AU - Heppner,D G, AU - Miller,R S, AU - Ockenhouse,C F, AU - Gubanov,A, AU - Shafer,R, AU - DeWitt,C C, AU - Quino-Ascurra,H A, AU - Kester,K E, AU - Kain,K C, AU - Walsh,D S, AU - Ballou,W R, AU - Gasser,R A,Jr PY - 2001/7/28/pubmed PY - 2002/1/5/medline PY - 2001/7/28/entrez SP - 2884 EP - 90 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 39 IS - 8 N2 - Microscopic detection of parasites has been the reference standard for malaria diagnosis for decades. However, difficulty in maintaining required technical skills and infrastructure has spurred the development of several nonmicroscopic malaria rapid diagnostic devices based on the detection of malaria parasite antigen in whole blood. The ParaSight F test is one such device. It detects the presence of Plasmodium falciparum-specific histidine-rich protein 2 by using an antigen-capture immunochromatographic strip format. The present study was conducted at outpatient malaria clinics in Iquitos, Peru, and Maesod, Thailand. Duplicate, blinded, expert microscopy was employed as the reference standard for evaluating device performance. Of 2,988 eligible patients, microscopy showed that 547 (18%) had P. falciparum, 658 (22%) had P. vivax, 2 (0.07%) had P. malariae, and 1,750 (59%) were negative for Plasmodium. Mixed infections (P. falciparum and P. vivax) were identified in 31 patients (1%). The overall sensitivity of ParaSight F for P. falciparum was 95%. When stratified by magnitude of parasitemia (no. of asexual parasites per microliter of whole blood), sensitivities were 83% (>0 to 500 parasites/microl), 87% (501 to 1,000/microl), 98% (1,001 to 5,000/microl), and 98% (>5,000/microl). Device specificity was 86%. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/11474008/Malaria_rapid_diagnostic_devices:_performance_characteristics_of_the_ParaSight_F_device_determined_in_a_multisite_field_study_ L2 - https://journals.asm.org/doi/10.1128/JCM.39.8.2884-2890.2001?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -