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[Effects of dexamethasone on apoptosis of airway inflammatory cells in asthmatic guinea-pigs].
Zhonghua Jie He He Hu Xi Za Zhi. 1998 Nov; 21(11):672-4.ZJ

Abstract

OBJECTIVE

Clearance of airway inflammatory cells is the key point of therapeutic effect in asthma. Apoptosis, a form of cell death, is thought to be critically important to promote the clearance of inflammatory cells and the resolution of inflammation. To investigate the effects of dexamethasone on apoptosis of airway inflammatory cells in asthma showed be very important.

METHOD

Dexamethasone was used for the treatment of asthma model of guinea pigs set up by inhaling ovalbumin. Apoptotic cells were labelled with TdT-mediated dUTP nick end labeling(TUNEL) technique on formalin-fixed paraffin-embedded trachea and lung tissue sections. By way of immunohistochemistry, eosinophils were stained with EG2 antibody and T lymphocytes with CD3+, CD4+ and CD8+ antibodies on sections.

RESULT

(1) The quantity of airway eosinophils and lymphocytes decreased in dexamethasone group, which could not be observed in the control one (P < 0.01). (2) Apoptotic index of lymphocytes was significantly elevated following dexamethasone treatment(P < 0.05). But no difference was found in the proportion of apoptotic eosinophils between these two groups. (3) The percentage of EG2 positive eosinophils and CD4+ positive lymphocytes decreased significantly following dexamethasone treatment. On the contrary, the number of CD8+ lymphocytes increased in dexamethasone group when compared with the control one (P < 0.05).

CONCLUSION

(1) The quantity of airway eosinophils decreases following dexamethasone treatment in guinea-pigs asthma, and its mechanism may not be ascribed mainly to the apoptosis of eosinophils. (2) The number of lymphocytes, mainly CD4+, decreased following dexamethasone treatment in guinea-pig airways, and apoptosis may represent the mechanism that promotes the clearance of lymphocytes in asthma.

Authors+Show Affiliations

Guangzhou Institute of Respiratory Disease, Guangzhou 510120.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

11477895

Citation

Ouyang, N, et al. "[Effects of Dexamethasone On Apoptosis of Airway Inflammatory Cells in Asthmatic Guinea-pigs]." Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, vol. 21, no. 11, 1998, pp. 672-4.
Ouyang N, Ding J, Chen S. [Effects of dexamethasone on apoptosis of airway inflammatory cells in asthmatic guinea-pigs]. Zhonghua Jie He He Hu Xi Za Zhi. 1998;21(11):672-4.
Ouyang, N., Ding, J., & Chen, S. (1998). [Effects of dexamethasone on apoptosis of airway inflammatory cells in asthmatic guinea-pigs]. Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, 21(11), 672-4.
Ouyang N, Ding J, Chen S. [Effects of Dexamethasone On Apoptosis of Airway Inflammatory Cells in Asthmatic Guinea-pigs]. Zhonghua Jie He He Hu Xi Za Zhi. 1998;21(11):672-4. PubMed PMID: 11477895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of dexamethasone on apoptosis of airway inflammatory cells in asthmatic guinea-pigs]. AU - Ouyang,N, AU - Ding,J, AU - Chen,S, PY - 2001/8/2/pubmed PY - 2001/9/21/medline PY - 2001/8/2/entrez SP - 672 EP - 4 JF - Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases JO - Zhonghua Jie He He Hu Xi Za Zhi VL - 21 IS - 11 N2 - OBJECTIVE: Clearance of airway inflammatory cells is the key point of therapeutic effect in asthma. Apoptosis, a form of cell death, is thought to be critically important to promote the clearance of inflammatory cells and the resolution of inflammation. To investigate the effects of dexamethasone on apoptosis of airway inflammatory cells in asthma showed be very important. METHOD: Dexamethasone was used for the treatment of asthma model of guinea pigs set up by inhaling ovalbumin. Apoptotic cells were labelled with TdT-mediated dUTP nick end labeling(TUNEL) technique on formalin-fixed paraffin-embedded trachea and lung tissue sections. By way of immunohistochemistry, eosinophils were stained with EG2 antibody and T lymphocytes with CD3+, CD4+ and CD8+ antibodies on sections. RESULT: (1) The quantity of airway eosinophils and lymphocytes decreased in dexamethasone group, which could not be observed in the control one (P < 0.01). (2) Apoptotic index of lymphocytes was significantly elevated following dexamethasone treatment(P < 0.05). But no difference was found in the proportion of apoptotic eosinophils between these two groups. (3) The percentage of EG2 positive eosinophils and CD4+ positive lymphocytes decreased significantly following dexamethasone treatment. On the contrary, the number of CD8+ lymphocytes increased in dexamethasone group when compared with the control one (P < 0.05). CONCLUSION: (1) The quantity of airway eosinophils decreases following dexamethasone treatment in guinea-pigs asthma, and its mechanism may not be ascribed mainly to the apoptosis of eosinophils. (2) The number of lymphocytes, mainly CD4+, decreased following dexamethasone treatment in guinea-pig airways, and apoptosis may represent the mechanism that promotes the clearance of lymphocytes in asthma. SN - 1001-0939 UR - https://www.unboundmedicine.com/medline/citation/11477895/[Effects_of_dexamethasone_on_apoptosis_of_airway_inflammatory_cells_in_asthmatic_guinea_pigs]_ L2 - https://medlineplus.gov/asthma.html DB - PRIME DP - Unbound Medicine ER -