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Effects of capsaicin on Ca(2+) release from the intracellular Ca(2+) stores in the dorsal root ganglion cells of adult rats.
Biochem Biophys Res Commun. 2001 Aug 03; 285(5):1114-20.BB

Abstract

We have investigated the effect of capsaicin on Ca(2+) release from the intracellular calcium stores. Intracellular calcium concentration ([Ca(2+)](i)) was measured in rat dorsal root ganglion (DRG) neurons using microfluorimetry with fura-2 indicator. Brief application of capsaicin (1 microM) elevated [Ca(2+)](i) in Ca(2+)-free solution. Capsaicin-induced [Ca(2+)](i) transient in Ca(2+)-free solution was evoked in a dose-dependent manner. Resiniferatoxin, an analogue of capsaicin, also raised [Ca(2+)](i) in Ca(2+)-free solution. Capsazepine, an antagonist of capsaicin receptor, completely blocked the capsaicin-induced [Ca(2+)](i) transient. Caffeine completely abolished capsaicin-induced [Ca(2+)](i) transient. Dantrolene sodium and ruthenium red, antagonists of the ryanodine receptor, blocked the effect of capsaicin on [Ca(2+)](i). However, capsaicin-induced [Ca(2+)](i) transient was not affected by 2-APB, a membrane-permeable IP(3) receptor antagonist. Furthermore, depletion of IP(3)-sensitive Ca(2+) stores by bradykinin and phospholipase C inhibitors, neomycin, and U-73122, did not block capsaicin-induced [Ca(2+)](i) transient. In conclusion, capsaicin increases [Ca(2+)](i) through Ca(2+) release from ryanodine-sensitive Ca(2+) stores, but not from IP(3)-sensitive Ca(2+) stores in addition to Ca(2+) entry through capsaicin-activated nonselective cation channel in rat DRG neurons.

Authors+Show Affiliations

Department of Physiology, Department of Biophysics, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul, 110-799, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11478769

Citation

Eun, S Y., et al. "Effects of Capsaicin On Ca(2+) Release From the Intracellular Ca(2+) Stores in the Dorsal Root Ganglion Cells of Adult Rats." Biochemical and Biophysical Research Communications, vol. 285, no. 5, 2001, pp. 1114-20.
Eun SY, Jung SJ, Park YK, et al. Effects of capsaicin on Ca(2+) release from the intracellular Ca(2+) stores in the dorsal root ganglion cells of adult rats. Biochem Biophys Res Commun. 2001;285(5):1114-20.
Eun, S. Y., Jung, S. J., Park, Y. K., Kwak, J., Kim, S. J., & Kim, J. (2001). Effects of capsaicin on Ca(2+) release from the intracellular Ca(2+) stores in the dorsal root ganglion cells of adult rats. Biochemical and Biophysical Research Communications, 285(5), 1114-20.
Eun SY, et al. Effects of Capsaicin On Ca(2+) Release From the Intracellular Ca(2+) Stores in the Dorsal Root Ganglion Cells of Adult Rats. Biochem Biophys Res Commun. 2001 Aug 3;285(5):1114-20. PubMed PMID: 11478769.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of capsaicin on Ca(2+) release from the intracellular Ca(2+) stores in the dorsal root ganglion cells of adult rats. AU - Eun,S Y, AU - Jung,S J, AU - Park,Y K, AU - Kwak,J, AU - Kim,S J, AU - Kim,J, PY - 2001/8/2/pubmed PY - 2001/9/14/medline PY - 2001/8/2/entrez SP - 1114 EP - 20 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 285 IS - 5 N2 - We have investigated the effect of capsaicin on Ca(2+) release from the intracellular calcium stores. Intracellular calcium concentration ([Ca(2+)](i)) was measured in rat dorsal root ganglion (DRG) neurons using microfluorimetry with fura-2 indicator. Brief application of capsaicin (1 microM) elevated [Ca(2+)](i) in Ca(2+)-free solution. Capsaicin-induced [Ca(2+)](i) transient in Ca(2+)-free solution was evoked in a dose-dependent manner. Resiniferatoxin, an analogue of capsaicin, also raised [Ca(2+)](i) in Ca(2+)-free solution. Capsazepine, an antagonist of capsaicin receptor, completely blocked the capsaicin-induced [Ca(2+)](i) transient. Caffeine completely abolished capsaicin-induced [Ca(2+)](i) transient. Dantrolene sodium and ruthenium red, antagonists of the ryanodine receptor, blocked the effect of capsaicin on [Ca(2+)](i). However, capsaicin-induced [Ca(2+)](i) transient was not affected by 2-APB, a membrane-permeable IP(3) receptor antagonist. Furthermore, depletion of IP(3)-sensitive Ca(2+) stores by bradykinin and phospholipase C inhibitors, neomycin, and U-73122, did not block capsaicin-induced [Ca(2+)](i) transient. In conclusion, capsaicin increases [Ca(2+)](i) through Ca(2+) release from ryanodine-sensitive Ca(2+) stores, but not from IP(3)-sensitive Ca(2+) stores in addition to Ca(2+) entry through capsaicin-activated nonselective cation channel in rat DRG neurons. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/11478769/Effects_of_capsaicin_on_Ca_2+__release_from_the_intracellular_Ca_2+__stores_in_the_dorsal_root_ganglion_cells_of_adult_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006291X01952726 DB - PRIME DP - Unbound Medicine ER -