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Improvement of HSV-1 necrotizing keratitis with amniotic membrane transplantation.
Invest Ophthalmol Vis Sci. 2001 Aug; 42(9):1969-74.IO

Abstract

PURPOSE

Stromal herpes simplex virus keratitis (HSK) is an immune-mediated disease. Previous studies have indicated that T cells, neutrophils, and macrophages contribute to the tissue damage in HSK. It has been shown that human amniotic membrane promotes epithelial wound healing and has diverse anti-inflammatory effects. In this study, the effect of amniotic membrane transplantation (AMT) on corneal wound healing and on inflammation in mice with necrotizing HSK was examined.

METHODS

BALB/c mice were corneally infected with 10(5) plaque-forming units (PFU) of HSV-1 (KOS strain). In 16 mice that exhibited severe ulcerating HSK, the cornea was covered with a preserved human amniotic membrane as a patch. Corneas in 16 infected mice remained uncovered and served as a control. On days 2 and 7 after surgery, the amniotic membrane was removed (eight mice in each group), the HSV-1-infected cornea was evaluated clinically, and the eye was enucleated. Tissue sections were analyzed histologically for epithelialization and cellular infiltration and immunohistochemically with anti-CD3 mAb to T cells, anti-CD11b mAb to both macrophages and neutrophils, or anti-F4/80 mAb to macrophages.

RESULTS

Profound regression of corneal inflammation and rapid closure of epithelial defects were observed clinically within 2 days in the amniotic membrane-covered eyes, whereas HSV-1 keratitis and ulceration progressed in all mice in the control group (P < 0.001). Histologically, corneal edema and inflammatory infiltration, and immunohistochemically the number of CD3(+), CD11b(+), and F4/80(+) cells in the cornea were markedly decreased at 2 and 7 days after amniotic membrane application, compared with the uncovered control corneas (P < 0.001).

CONCLUSIONS

AMT promotes rapid epithelialization and reduces stromal inflammation and ulceration in HSV-1 keratitis. AMT in mice with HSV necrotizing stromal keratitis appears to be a useful model for investigating the effect and the action mechanism of human amniotic membrane.

Authors+Show Affiliations

Department of Ophthalmology, University of Essen, Germany. arnd.heligenhaus@t-online.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11481259

Citation

Heiligenhaus, A, et al. "Improvement of HSV-1 Necrotizing Keratitis With Amniotic Membrane Transplantation." Investigative Ophthalmology & Visual Science, vol. 42, no. 9, 2001, pp. 1969-74.
Heiligenhaus A, Bauer D, Meller D, et al. Improvement of HSV-1 necrotizing keratitis with amniotic membrane transplantation. Invest Ophthalmol Vis Sci. 2001;42(9):1969-74.
Heiligenhaus, A., Bauer, D., Meller, D., Steuhl, K. P., & Tseng, S. C. (2001). Improvement of HSV-1 necrotizing keratitis with amniotic membrane transplantation. Investigative Ophthalmology & Visual Science, 42(9), 1969-74.
Heiligenhaus A, et al. Improvement of HSV-1 Necrotizing Keratitis With Amniotic Membrane Transplantation. Invest Ophthalmol Vis Sci. 2001;42(9):1969-74. PubMed PMID: 11481259.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improvement of HSV-1 necrotizing keratitis with amniotic membrane transplantation. AU - Heiligenhaus,A, AU - Bauer,D, AU - Meller,D, AU - Steuhl,K P, AU - Tseng,S C, PY - 2001/8/2/pubmed PY - 2001/8/17/medline PY - 2001/8/2/entrez SP - 1969 EP - 74 JF - Investigative ophthalmology & visual science JO - Invest. Ophthalmol. Vis. Sci. VL - 42 IS - 9 N2 - PURPOSE: Stromal herpes simplex virus keratitis (HSK) is an immune-mediated disease. Previous studies have indicated that T cells, neutrophils, and macrophages contribute to the tissue damage in HSK. It has been shown that human amniotic membrane promotes epithelial wound healing and has diverse anti-inflammatory effects. In this study, the effect of amniotic membrane transplantation (AMT) on corneal wound healing and on inflammation in mice with necrotizing HSK was examined. METHODS: BALB/c mice were corneally infected with 10(5) plaque-forming units (PFU) of HSV-1 (KOS strain). In 16 mice that exhibited severe ulcerating HSK, the cornea was covered with a preserved human amniotic membrane as a patch. Corneas in 16 infected mice remained uncovered and served as a control. On days 2 and 7 after surgery, the amniotic membrane was removed (eight mice in each group), the HSV-1-infected cornea was evaluated clinically, and the eye was enucleated. Tissue sections were analyzed histologically for epithelialization and cellular infiltration and immunohistochemically with anti-CD3 mAb to T cells, anti-CD11b mAb to both macrophages and neutrophils, or anti-F4/80 mAb to macrophages. RESULTS: Profound regression of corneal inflammation and rapid closure of epithelial defects were observed clinically within 2 days in the amniotic membrane-covered eyes, whereas HSV-1 keratitis and ulceration progressed in all mice in the control group (P < 0.001). Histologically, corneal edema and inflammatory infiltration, and immunohistochemically the number of CD3(+), CD11b(+), and F4/80(+) cells in the cornea were markedly decreased at 2 and 7 days after amniotic membrane application, compared with the uncovered control corneas (P < 0.001). CONCLUSIONS: AMT promotes rapid epithelialization and reduces stromal inflammation and ulceration in HSV-1 keratitis. AMT in mice with HSV necrotizing stromal keratitis appears to be a useful model for investigating the effect and the action mechanism of human amniotic membrane. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/11481259/Improvement_of_HSV_1_necrotizing_keratitis_with_amniotic_membrane_transplantation_ L2 - http://iovs.arvojournals.org/article.aspx?volume=42&amp;page=1969 DB - PRIME DP - Unbound Medicine ER -