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Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction.
J Virol Methods. 2001 Sep; 97(1-2):59-66.JV

Abstract

An RT-PCR-hybridization was developed that amplified genetic material from the M protein gene of HCoV-229E and HCoV-OC43. The analytic sensitivity of these original primers were compared with primers defined in the N gene and described previously. The results show that 0.05 TCID50 of HCoV-229E and 0.01 TCID50 of HCoV-OC43 can be detected by this molecular method using the original method. Detection of HCoV-229E and HCoV-OC43 in clinical specimens is possible using this method: 348 respiratory specimens (202 sputum and 146 nasal aspirates) were tested with this RT-PCR-hybridization and 12 human coronavirus are detected (3%). The method could provide a useful tool for demonstrating the role of human coronavirus in infections of the respiratory tract.

Authors+Show Affiliations

Laboratory of Human and Molecular Virology, University Hopital, av. Georges Clemenceau, 14 033, Caen, France. vabret-a@chu-caen.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11483217

Citation

Vabret, A, et al. "Direct Diagnosis of Human Respiratory Coronaviruses 229E and OC43 By the Polymerase Chain Reaction." Journal of Virological Methods, vol. 97, no. 1-2, 2001, pp. 59-66.
Vabret A, Mouthon F, Mourez T, et al. Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction. J Virol Methods. 2001;97(1-2):59-66.
Vabret, A., Mouthon, F., Mourez, T., Gouarin, S., Petitjean, J., & Freymuth, F. (2001). Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction. Journal of Virological Methods, 97(1-2), 59-66.
Vabret A, et al. Direct Diagnosis of Human Respiratory Coronaviruses 229E and OC43 By the Polymerase Chain Reaction. J Virol Methods. 2001;97(1-2):59-66. PubMed PMID: 11483217.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction. AU - Vabret,A, AU - Mouthon,F, AU - Mourez,T, AU - Gouarin,S, AU - Petitjean,J, AU - Freymuth,F, PY - 2001/8/3/pubmed PY - 2002/5/16/medline PY - 2001/8/3/entrez SP - 59 EP - 66 JF - Journal of virological methods JO - J Virol Methods VL - 97 IS - 1-2 N2 - An RT-PCR-hybridization was developed that amplified genetic material from the M protein gene of HCoV-229E and HCoV-OC43. The analytic sensitivity of these original primers were compared with primers defined in the N gene and described previously. The results show that 0.05 TCID50 of HCoV-229E and 0.01 TCID50 of HCoV-OC43 can be detected by this molecular method using the original method. Detection of HCoV-229E and HCoV-OC43 in clinical specimens is possible using this method: 348 respiratory specimens (202 sputum and 146 nasal aspirates) were tested with this RT-PCR-hybridization and 12 human coronavirus are detected (3%). The method could provide a useful tool for demonstrating the role of human coronavirus in infections of the respiratory tract. SN - 0166-0934 UR - https://www.unboundmedicine.com/medline/citation/11483217/Direct_diagnosis_of_human_respiratory_coronaviruses_229E_and_OC43_by_the_polymerase_chain_reaction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166093401003433 DB - PRIME DP - Unbound Medicine ER -