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Neuronal expression of mint1 and mint2, novel multimodular proteins, in adult murine brain.
Brain Res Mol Brain Res. 2001 Aug 15; 92(1-2):27-42.BR

Abstract

Mints are multimodular adapter proteins in functioning membrane transport and organization. Mint1 and mint2 are neuron-specific. We localized these isoforms in mouse brain. By in situ hybridization, mRNA encoding mint1 or mint2 was expressed in neurons throughout the brain. Mint1 mRNA expression was greatest in the limbic system including cingulate cortex, hippocampus, anterior thalamic nuclei, medial habenular nucleus, and mammillary body. Mint2 mRNA was rich in cerebral cortex, entorhinal cortex, and hippocampus, but less prominent in other limbic structures. Mint1 mRNA and mint2 mRNA were distributed among hippocampal pyramidal neurons, while mint2 mRNA was especially abundant in CA3. Mint1, but not Mint2 mRNA was abundant in the substantia nigra pars compacta. Immunohistochemistry visualized mint proteins in axon terminals and neuronal somata, generally following mRNA distribution. In the hippocampus, mint1 was rich in the entorhinal projections and mossy fibers of the dentate gyrus, while mint2 was rich in commisural fibers from the contralateral hippocampus and in CA1. Mint1 intensely stained catecholamine-containing neurons such as the substantia nigra pars compacta, ventral tegmental area, and locus ceruleus. Mint2 protein was ubiquitous in these regions. Mint1 and mint2 distribution also differed elsewhere in the brainstem and in the cerebellum. Central nervous system neurons, then, predominantly express either mint1 or mint2. Mints may be involved in synaptic vesicle transport toward the active zone, also participating in transport of certain membrane proteins toward the postsynaptic density. Mint1 and mint2 may divide roles either regionally or depending on neuronal functional characteristics.

Authors+Show Affiliations

Department of Internal Medicine, Hyogo College of Medicine, 1-1 Mukogawacho, Nishinomiya 663-8501, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11483239

Citation

Nakajima, Y, et al. "Neuronal Expression of Mint1 and Mint2, Novel Multimodular Proteins, in Adult Murine Brain." Brain Research. Molecular Brain Research, vol. 92, no. 1-2, 2001, pp. 27-42.
Nakajima Y, Okamoto M, Nishimura H, et al. Neuronal expression of mint1 and mint2, novel multimodular proteins, in adult murine brain. Brain Res Mol Brain Res. 2001;92(1-2):27-42.
Nakajima, Y., Okamoto, M., Nishimura, H., Obata, K., Kitano, H., Sugita, M., & Matsuyama, T. (2001). Neuronal expression of mint1 and mint2, novel multimodular proteins, in adult murine brain. Brain Research. Molecular Brain Research, 92(1-2), 27-42.
Nakajima Y, et al. Neuronal Expression of Mint1 and Mint2, Novel Multimodular Proteins, in Adult Murine Brain. Brain Res Mol Brain Res. 2001 Aug 15;92(1-2):27-42. PubMed PMID: 11483239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuronal expression of mint1 and mint2, novel multimodular proteins, in adult murine brain. AU - Nakajima,Y, AU - Okamoto,M, AU - Nishimura,H, AU - Obata,K, AU - Kitano,H, AU - Sugita,M, AU - Matsuyama,T, PY - 2001/8/3/pubmed PY - 2002/1/5/medline PY - 2001/8/3/entrez SP - 27 EP - 42 JF - Brain research. Molecular brain research JO - Brain Res. Mol. Brain Res. VL - 92 IS - 1-2 N2 - Mints are multimodular adapter proteins in functioning membrane transport and organization. Mint1 and mint2 are neuron-specific. We localized these isoforms in mouse brain. By in situ hybridization, mRNA encoding mint1 or mint2 was expressed in neurons throughout the brain. Mint1 mRNA expression was greatest in the limbic system including cingulate cortex, hippocampus, anterior thalamic nuclei, medial habenular nucleus, and mammillary body. Mint2 mRNA was rich in cerebral cortex, entorhinal cortex, and hippocampus, but less prominent in other limbic structures. Mint1 mRNA and mint2 mRNA were distributed among hippocampal pyramidal neurons, while mint2 mRNA was especially abundant in CA3. Mint1, but not Mint2 mRNA was abundant in the substantia nigra pars compacta. Immunohistochemistry visualized mint proteins in axon terminals and neuronal somata, generally following mRNA distribution. In the hippocampus, mint1 was rich in the entorhinal projections and mossy fibers of the dentate gyrus, while mint2 was rich in commisural fibers from the contralateral hippocampus and in CA1. Mint1 intensely stained catecholamine-containing neurons such as the substantia nigra pars compacta, ventral tegmental area, and locus ceruleus. Mint2 protein was ubiquitous in these regions. Mint1 and mint2 distribution also differed elsewhere in the brainstem and in the cerebellum. Central nervous system neurons, then, predominantly express either mint1 or mint2. Mints may be involved in synaptic vesicle transport toward the active zone, also participating in transport of certain membrane proteins toward the postsynaptic density. Mint1 and mint2 may divide roles either regionally or depending on neuronal functional characteristics. SN - 0169-328X UR - https://www.unboundmedicine.com/medline/citation/11483239/Neuronal_expression_of_mint1_and_mint2_novel_multimodular_proteins_in_adult_murine_brain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0169328X01001267 DB - PRIME DP - Unbound Medicine ER -