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Determination of ephedrines in urine by gas chromatography-mass spectrometry.
J Chromatogr B Biomed Sci Appl. 2001 Jul 15; 758(2):311-4.JC

Abstract

A selective gas-liquid chromatographic method with mass spectrometry (GC-MS) for the simultaneous confirmation and quantification of ephedrine, pseudo-ephedrine, nor-ephedrine, nor-pseudoephedrine, which are pairs of diastereoisomeric sympathomimetic amines, and methyl-ephedrine was developed for doping control analysis in urine samples. O-Trimethylsilylated and N-mono-trifluoroacetylated derivatives of ephedrines--one derivative was formed for each ephedrine--were prepared and analyzed by GC-MS, after alkaline extraction of urine and evaporation of the organic phase, using d3-ephedrine as internal standard. Calibration curves, with r2>0.98, ranged from 3.0 to 50 microg/ml depending on the analyte. Validation data (specificity, % RSD, accuracy, and recovery) are also presented.

Authors+Show Affiliations

Doppling Control Laboratory of Athens, Olympic Athletic Center of Athens Spiros Louis, Maroussi, Greece.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11486842

Citation

Spyridaki, M H., et al. "Determination of Ephedrines in Urine By Gas Chromatography-mass Spectrometry." Journal of Chromatography. B, Biomedical Sciences and Applications, vol. 758, no. 2, 2001, pp. 311-4.
Spyridaki MH, Tsitsimpikou CJ, Siskos PA, et al. Determination of ephedrines in urine by gas chromatography-mass spectrometry. J Chromatogr B Biomed Sci Appl. 2001;758(2):311-4.
Spyridaki, M. H., Tsitsimpikou, C. J., Siskos, P. A., & Georgakopoulos, C. G. (2001). Determination of ephedrines in urine by gas chromatography-mass spectrometry. Journal of Chromatography. B, Biomedical Sciences and Applications, 758(2), 311-4.
Spyridaki MH, et al. Determination of Ephedrines in Urine By Gas Chromatography-mass Spectrometry. J Chromatogr B Biomed Sci Appl. 2001 Jul 15;758(2):311-4. PubMed PMID: 11486842.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of ephedrines in urine by gas chromatography-mass spectrometry. AU - Spyridaki,M H, AU - Tsitsimpikou,C J, AU - Siskos,P A, AU - Georgakopoulos,C G, PY - 2001/8/7/pubmed PY - 2002/1/5/medline PY - 2001/8/7/entrez SP - 311 EP - 4 JF - Journal of chromatography. B, Biomedical sciences and applications JO - J Chromatogr B Biomed Sci Appl VL - 758 IS - 2 N2 - A selective gas-liquid chromatographic method with mass spectrometry (GC-MS) for the simultaneous confirmation and quantification of ephedrine, pseudo-ephedrine, nor-ephedrine, nor-pseudoephedrine, which are pairs of diastereoisomeric sympathomimetic amines, and methyl-ephedrine was developed for doping control analysis in urine samples. O-Trimethylsilylated and N-mono-trifluoroacetylated derivatives of ephedrines--one derivative was formed for each ephedrine--were prepared and analyzed by GC-MS, after alkaline extraction of urine and evaporation of the organic phase, using d3-ephedrine as internal standard. Calibration curves, with r2>0.98, ranged from 3.0 to 50 microg/ml depending on the analyte. Validation data (specificity, % RSD, accuracy, and recovery) are also presented. SN - 1387-2273 UR - https://www.unboundmedicine.com/medline/citation/11486842/Determination_of_ephedrines_in_urine_by_gas_chromatography_mass_spectrometry_ L2 - https://antibodies.cancer.gov/detail/CPTC-RRM2-2 DB - PRIME DP - Unbound Medicine ER -