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Role of MOG-stimulated Th1 type "light up" (GFP+) CD4+ T cells for the development of experimental autoimmune encephalomyelitis (EAE).
J Autoimmun. 2001 Aug; 17(1):17-25.JA

Abstract

Experimental autoimmune encephalomyelitis (EAE) is an animal model for multiple sclerosis in humans. EAE can be passively transferred into naive syngeneic animals by administration of MOG-specific T cell clones. Lymphocytes isolated from green fluorescent protein (GFP)-transgenic (Tg) mice can light up by emitting green fluorescence, thus making it feasible to use such animals in a passive transfer model for EAE. When MOG-sensitized splenic lymphocytes from GFP-Tg mice were adoptively transferred to irradiated, syngeneic C57BL/6 and RAG-1(-/-)mice, typical symptoms of EAE developed. Analysis of the reconstituted mice with EAE revealed prominent infiltration of fluorescing (GFP+), CD4+ T cells into the central nervous system (CNS). Real-time confocal imaging revealed these cells in the spinal cords and brains of recipient mice. This infiltration was also confirmed by anti-GFP monoclonal antibodies. Furthermore, quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) evaluation indicated that the infiltrating GFP+, CD4+ T cells exclusively produced T helper type 1 (Th1) cytokines, especially interferon-gamma (IFN-gamma). These results clearly show that MOG-specific CD4+ T cells preferentially invade into the CNS and mediate the development of EAE by producing Th1-biased cytokines.

Authors+Show Affiliations

Department of Mucosal Immunology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11488634

Citation

Yura, M, et al. "Role of MOG-stimulated Th1 Type "light Up" (GFP+) CD4+ T Cells for the Development of Experimental Autoimmune Encephalomyelitis (EAE)." Journal of Autoimmunity, vol. 17, no. 1, 2001, pp. 17-25.
Yura M, Takahashi I, Serada M, et al. Role of MOG-stimulated Th1 type "light up" (GFP+) CD4+ T cells for the development of experimental autoimmune encephalomyelitis (EAE). J Autoimmun. 2001;17(1):17-25.
Yura, M., Takahashi, I., Serada, M., Koshio, T., Nakagami, K., Yuki, Y., & Kiyono, H. (2001). Role of MOG-stimulated Th1 type "light up" (GFP+) CD4+ T cells for the development of experimental autoimmune encephalomyelitis (EAE). Journal of Autoimmunity, 17(1), 17-25.
Yura M, et al. Role of MOG-stimulated Th1 Type "light Up" (GFP+) CD4+ T Cells for the Development of Experimental Autoimmune Encephalomyelitis (EAE). J Autoimmun. 2001;17(1):17-25. PubMed PMID: 11488634.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of MOG-stimulated Th1 type "light up" (GFP+) CD4+ T cells for the development of experimental autoimmune encephalomyelitis (EAE). AU - Yura,M, AU - Takahashi,I, AU - Serada,M, AU - Koshio,T, AU - Nakagami,K, AU - Yuki,Y, AU - Kiyono,H, PY - 2001/8/8/pubmed PY - 2002/1/5/medline PY - 2001/8/8/entrez SP - 17 EP - 25 JF - Journal of autoimmunity JO - J Autoimmun VL - 17 IS - 1 N2 - Experimental autoimmune encephalomyelitis (EAE) is an animal model for multiple sclerosis in humans. EAE can be passively transferred into naive syngeneic animals by administration of MOG-specific T cell clones. Lymphocytes isolated from green fluorescent protein (GFP)-transgenic (Tg) mice can light up by emitting green fluorescence, thus making it feasible to use such animals in a passive transfer model for EAE. When MOG-sensitized splenic lymphocytes from GFP-Tg mice were adoptively transferred to irradiated, syngeneic C57BL/6 and RAG-1(-/-)mice, typical symptoms of EAE developed. Analysis of the reconstituted mice with EAE revealed prominent infiltration of fluorescing (GFP+), CD4+ T cells into the central nervous system (CNS). Real-time confocal imaging revealed these cells in the spinal cords and brains of recipient mice. This infiltration was also confirmed by anti-GFP monoclonal antibodies. Furthermore, quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) evaluation indicated that the infiltrating GFP+, CD4+ T cells exclusively produced T helper type 1 (Th1) cytokines, especially interferon-gamma (IFN-gamma). These results clearly show that MOG-specific CD4+ T cells preferentially invade into the CNS and mediate the development of EAE by producing Th1-biased cytokines. SN - 0896-8411 UR - https://www.unboundmedicine.com/medline/citation/11488634/Role_of_MOG_stimulated_Th1_type_"light_up"__GFP+__CD4+_T_cells_for_the_development_of_experimental_autoimmune_encephalomyelitis__EAE__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0896-8411(01)90520-7 DB - PRIME DP - Unbound Medicine ER -