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Assay of galactose-1-phosphate uridyl transferase in cultured amniotic cells for prenatal diagnosis of galactosaemia.
Clin Chim Acta. 1975 Jul 23; 62(2):189-94.CC

Abstract

We report studies designed to establish optimal conditions for the assay of amniotic cell galactose 1-phosphate uridyl transferase (Gal-PUT) for early prenatal diagnosis of galactosaemia. Methods based on linkage of the reaction to cause of non-specific reactions occurring even in the absence of Gal-1-P. In the final method, sonicates of confluent cultures are incubated with (14-C) Gal-1-P is degraded by treatment with alkaline phosphatase. Gal-PUT specific activities of both control and galactosaemic amniotic cells are higher in non-confluent that confluent cultures.

Authors

Fensom AH
No affiliation info available
Benson PF
No affiliation info available

MeSH

AmniocentesisAmniotic FluidCells, CulturedFemaleGalactosemiasHumansKineticsMethodsNucleotidyltransferasesPregnancyPrenatal DiagnosisTime FactorsUTP-Hexose-1-Phosphate Uridylyltransferase

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1149286

Citation

Fensom, A H., and P F. Benson. "Assay of Galactose-1-phosphate Uridyl Transferase in Cultured Amniotic Cells for Prenatal Diagnosis of Galactosaemia." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 62, no. 2, 1975, pp. 189-94.
Fensom AH, Benson PF. Assay of galactose-1-phosphate uridyl transferase in cultured amniotic cells for prenatal diagnosis of galactosaemia. Clin Chim Acta. 1975;62(2):189-94.
Fensom, A. H., & Benson, P. F. (1975). Assay of galactose-1-phosphate uridyl transferase in cultured amniotic cells for prenatal diagnosis of galactosaemia. Clinica Chimica Acta; International Journal of Clinical Chemistry, 62(2), 189-94.
Fensom AH, Benson PF. Assay of Galactose-1-phosphate Uridyl Transferase in Cultured Amniotic Cells for Prenatal Diagnosis of Galactosaemia. Clin Chim Acta. 1975 Jul 23;62(2):189-94. PubMed PMID: 1149286.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assay of galactose-1-phosphate uridyl transferase in cultured amniotic cells for prenatal diagnosis of galactosaemia. AU - Fensom,A H, AU - Benson,P F, PY - 1975/7/23/pubmed PY - 1975/7/23/medline PY - 1975/7/23/entrez SP - 189 EP - 94 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin Chim Acta VL - 62 IS - 2 N2 - We report studies designed to establish optimal conditions for the assay of amniotic cell galactose 1-phosphate uridyl transferase (Gal-PUT) for early prenatal diagnosis of galactosaemia. Methods based on linkage of the reaction to cause of non-specific reactions occurring even in the absence of Gal-1-P. In the final method, sonicates of confluent cultures are incubated with (14-C) Gal-1-P is degraded by treatment with alkaline phosphatase. Gal-PUT specific activities of both control and galactosaemic amniotic cells are higher in non-confluent that confluent cultures. SN - 0009-8981 UR - https://www.unboundmedicine.com/medline/citation/1149286/Assay_of_galactose_1_phosphate_uridyl_transferase_in_cultured_amniotic_cells_for_prenatal_diagnosis_of_galactosaemia_ DB - PRIME DP - Unbound Medicine ER -