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Expression profile of nine novel genes differentially expressed in hepatitis B virus-associated hepatocellular carcinomas.
Oncogene. 2001 Jul 27; 20(33):4568-75.O

Abstract

Chronic hepatitis B virus (HBV) infection is known to be one of the major causes in the development of hepatocellular carcinoma (HCC), although the biomolecular mechanism(s) involved remain unclear. To identify the cellular gene(s) involved in HBV-associated hepatocarcinogenesis, we used the mRNA differential display method and examined three paired tumor and nontumor tissues, all of which had chromosomally integrated HBV-DNA through chronic infection. Using 240 different combinations of three one-base anchored oligo-dT primers and 80 arbitrary 13-mers, genes decreased or increased in expression more than twofold between each tumor tissue and its paired nontumor tissue were identified. Twenty-nine known genes and four novel genes were differentially over-expressed in the HCC tumor tissues. In contrast, 27 known genes and five novel genes were under-expressed in those tumor tissues. The nucleotide sequences of the nine novel gene fragments were determined and their expression patterns were examined in 40 HCC samples. HA61T2, PT18, HG63T1, and HG57T1 were preferentially over-expressed in 32 cases (80%, P<0.001), 24 cases (60%), 23 cases (57.5%) and 22 cases (55%) of the 40 tumor tissues, respectively. There was an increased frequency of HG57T1 over-expression in HCC patients with HBV-positive serology and low serum alpha-feto protein (AFP) levels (P<0.05). DNT10, PT8, PT19, ENT25 and HA6T4 were under-expressed in 26 cases (65%), 23 cases (57.5%), 21 cases (53%), 20 cases (50%) and 18 cases (45%) of the 40 tumor samples, respectively. There was a strong correlation of DNT10 under-expression with high serum AFP level in HCC patients, irrespective of HBV serology (P<0.01). HA6T4 was preferentially under-expressed in HCC tumors in patients with HBV-positive serology and high serum AFP levels (P<0.05). Thus, the functional analyses of the known and novel genes identified in this study should prove valuable to further understand the mechanism(s) of hepatocarcinogenesis.

Authors+Show Affiliations

Department of Internal Medicine, Division of GI and Hepatology, Research Institute of Clinical Medicine, Chonbuk National University Medical School and Hospital, Chonju, Chonbuk 561-712, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11494152

Citation

Kim, M Y., et al. "Expression Profile of Nine Novel Genes Differentially Expressed in Hepatitis B Virus-associated Hepatocellular Carcinomas." Oncogene, vol. 20, no. 33, 2001, pp. 4568-75.
Kim MY, Park E, Park JH, et al. Expression profile of nine novel genes differentially expressed in hepatitis B virus-associated hepatocellular carcinomas. Oncogene. 2001;20(33):4568-75.
Kim, M. Y., Park, E., Park, J. H., Park, D. H., Moon, W. S., Cho, B. H., Shin, H. S., & Kim, D. G. (2001). Expression profile of nine novel genes differentially expressed in hepatitis B virus-associated hepatocellular carcinomas. Oncogene, 20(33), 4568-75.
Kim MY, et al. Expression Profile of Nine Novel Genes Differentially Expressed in Hepatitis B Virus-associated Hepatocellular Carcinomas. Oncogene. 2001 Jul 27;20(33):4568-75. PubMed PMID: 11494152.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression profile of nine novel genes differentially expressed in hepatitis B virus-associated hepatocellular carcinomas. AU - Kim,M Y, AU - Park,E, AU - Park,J H, AU - Park,D H, AU - Moon,W S, AU - Cho,B H, AU - Shin,H S, AU - Kim,D G, PY - 2001/03/19/received PY - 2001/04/26/revised PY - 2001/05/08/accepted PY - 2001/8/9/pubmed PY - 2001/9/8/medline PY - 2001/8/9/entrez SP - 4568 EP - 75 JF - Oncogene JO - Oncogene VL - 20 IS - 33 N2 - Chronic hepatitis B virus (HBV) infection is known to be one of the major causes in the development of hepatocellular carcinoma (HCC), although the biomolecular mechanism(s) involved remain unclear. To identify the cellular gene(s) involved in HBV-associated hepatocarcinogenesis, we used the mRNA differential display method and examined three paired tumor and nontumor tissues, all of which had chromosomally integrated HBV-DNA through chronic infection. Using 240 different combinations of three one-base anchored oligo-dT primers and 80 arbitrary 13-mers, genes decreased or increased in expression more than twofold between each tumor tissue and its paired nontumor tissue were identified. Twenty-nine known genes and four novel genes were differentially over-expressed in the HCC tumor tissues. In contrast, 27 known genes and five novel genes were under-expressed in those tumor tissues. The nucleotide sequences of the nine novel gene fragments were determined and their expression patterns were examined in 40 HCC samples. HA61T2, PT18, HG63T1, and HG57T1 were preferentially over-expressed in 32 cases (80%, P<0.001), 24 cases (60%), 23 cases (57.5%) and 22 cases (55%) of the 40 tumor tissues, respectively. There was an increased frequency of HG57T1 over-expression in HCC patients with HBV-positive serology and low serum alpha-feto protein (AFP) levels (P<0.05). DNT10, PT8, PT19, ENT25 and HA6T4 were under-expressed in 26 cases (65%), 23 cases (57.5%), 21 cases (53%), 20 cases (50%) and 18 cases (45%) of the 40 tumor samples, respectively. There was a strong correlation of DNT10 under-expression with high serum AFP level in HCC patients, irrespective of HBV serology (P<0.01). HA6T4 was preferentially under-expressed in HCC tumors in patients with HBV-positive serology and high serum AFP levels (P<0.05). Thus, the functional analyses of the known and novel genes identified in this study should prove valuable to further understand the mechanism(s) of hepatocarcinogenesis. SN - 0950-9232 UR - https://www.unboundmedicine.com/medline/citation/11494152/Expression_profile_of_nine_novel_genes_differentially_expressed_in_hepatitis_B_virus_associated_hepatocellular_carcinomas_ L2 - http://dx.doi.org/10.1038/sj.onc.1204626 DB - PRIME DP - Unbound Medicine ER -