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The expression of plasminogen activator system in a rat model of periodontal wound healing.
J Periodontol. 2001 Jul; 72(7):849-57.JP

Abstract

BACKGROUND

The plasminogen activator system has been proposed to play a role in proteolytic degradation of extracellular matrices in tissue remodeling, including wound healing. The aim of this study was to elucidate the presence of components of the plasminogen activator system during different stages of periodontal wound healing.

METHODS

Periodontal wounds were created around the molars of adult rats and healing was followed for 28 days. Immunohistochemical analyses of the healing tissues and an analysis of the periodontal wound healing fluid by ELISA were carried out for the detection of tissue-type plasminogen activator (t-PA), urokinase-type plasminogen activator (u-PA), and 2 plasminogen activator inhibitors (PAI-1 and PAI-2).

RESULTS

During the early stages (days 1 to 3) of periodontal wound healing, PAI-1 and PAI-2 were found to be closely associated with the deposition of a fibrin clot in the gingival sulcus. These components were strongly associated with the infiltrating inflammatory cells around the fibrin clot. During days 3 to 7, u-PA, PAI-1, and PAI-2 were associated with cells (particularly monocytes/macrophages, fibroblasts, and endothelial cells) in the newly formed granulation tissue. During days 7 to 14, a new attachment apparatus was formed during which PAI-1, PAI-2, and u-PA were localized in both periodontal ligament fibroblasts (PDL) and epithelial cells at sites where these cells were attaching to the root surface. In the periodontal wound healing fluid, the concentration for t-PA increased and peaked during the first week. PAI-2 had a similar expression to t-PA, but at a lower level over the entire wound-healing period.

CONCLUSIONS

These findings indicate that the plasminogen activator system is involved in the entire process of periodontal wound healing, in particular with the formation of fibrin matrix on the root surface and its replacement by granulation tissue, as well as the subsequent formation of the attachment of soft tissue to the root surface during the later stages of wound repair.

Authors+Show Affiliations

School of Dentistry, The University of Queensland, Brisbane, Australia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11495131

Citation

Xiao, Y, et al. "The Expression of Plasminogen Activator System in a Rat Model of Periodontal Wound Healing." Journal of Periodontology, vol. 72, no. 7, 2001, pp. 849-57.
Xiao Y, Li H, Bunn C, et al. The expression of plasminogen activator system in a rat model of periodontal wound healing. J Periodontol. 2001;72(7):849-57.
Xiao, Y., Li, H., Bunn, C., & Bartold, P. M. (2001). The expression of plasminogen activator system in a rat model of periodontal wound healing. Journal of Periodontology, 72(7), 849-57.
Xiao Y, et al. The Expression of Plasminogen Activator System in a Rat Model of Periodontal Wound Healing. J Periodontol. 2001;72(7):849-57. PubMed PMID: 11495131.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The expression of plasminogen activator system in a rat model of periodontal wound healing. AU - Xiao,Y, AU - Li,H, AU - Bunn,C, AU - Bartold,P M, PY - 2001/8/10/pubmed PY - 2001/10/26/medline PY - 2001/8/10/entrez SP - 849 EP - 57 JF - Journal of periodontology JO - J Periodontol VL - 72 IS - 7 N2 - BACKGROUND: The plasminogen activator system has been proposed to play a role in proteolytic degradation of extracellular matrices in tissue remodeling, including wound healing. The aim of this study was to elucidate the presence of components of the plasminogen activator system during different stages of periodontal wound healing. METHODS: Periodontal wounds were created around the molars of adult rats and healing was followed for 28 days. Immunohistochemical analyses of the healing tissues and an analysis of the periodontal wound healing fluid by ELISA were carried out for the detection of tissue-type plasminogen activator (t-PA), urokinase-type plasminogen activator (u-PA), and 2 plasminogen activator inhibitors (PAI-1 and PAI-2). RESULTS: During the early stages (days 1 to 3) of periodontal wound healing, PAI-1 and PAI-2 were found to be closely associated with the deposition of a fibrin clot in the gingival sulcus. These components were strongly associated with the infiltrating inflammatory cells around the fibrin clot. During days 3 to 7, u-PA, PAI-1, and PAI-2 were associated with cells (particularly monocytes/macrophages, fibroblasts, and endothelial cells) in the newly formed granulation tissue. During days 7 to 14, a new attachment apparatus was formed during which PAI-1, PAI-2, and u-PA were localized in both periodontal ligament fibroblasts (PDL) and epithelial cells at sites where these cells were attaching to the root surface. In the periodontal wound healing fluid, the concentration for t-PA increased and peaked during the first week. PAI-2 had a similar expression to t-PA, but at a lower level over the entire wound-healing period. CONCLUSIONS: These findings indicate that the plasminogen activator system is involved in the entire process of periodontal wound healing, in particular with the formation of fibrin matrix on the root surface and its replacement by granulation tissue, as well as the subsequent formation of the attachment of soft tissue to the root surface during the later stages of wound repair. SN - 0022-3492 UR - https://www.unboundmedicine.com/medline/citation/11495131/The_expression_of_plasminogen_activator_system_in_a_rat_model_of_periodontal_wound_healing_ L2 - https://doi.org/10.1902/jop.2001.72.7.849 DB - PRIME DP - Unbound Medicine ER -