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Doenjang hexane fraction-induced G1 arrest is associated with the inhibition of pRB phosphorylation and induction of Cdk inhibitor p21 in human breast carcinoma MCF-7 cells.
Oncol Rep. 2001 Sep-Oct; 8(5):1091-6.OR

Abstract

Doenjang (fermented soy paste) is a Korean traditional fermented food. In previous studies, we demonstrated that Doenjang extracts exhibited anti-mutagenic and anti-carcinogenic activities. Here we investigated the effects of Doenjang hexane fraction (DHF) on cell cycle progression in the human breast carcinoma MCF-7 cells. Treatment of DHF to MCF-7 cells induced a G1 phase arrest of the cell cycle, which correlated with the accumulation of the hypophosphorylated form of the retinoblastoma protein (pRB) and enhanced association of pRB with the transcription factor E2F-1. After DHF treatment, the expression of D-type cyclins was decreased in a time-dependent manner, but DHF did not affect the levels of cyclin-dependent kinases (Cdks), cyclin E and cyclin A protein. However, the activity of Cdk2 and cyclin E-associated kinase was decreased in a time-dependent manner. The tumor suppressor p53 and Cdk inhibitor p21, a known downstream effector of the p53, and association of p21 with Cdk2 were markedly induced in DHF-treated cells. Taken together, the present results indicate that treatment of MCF-7 cells with DHF induces a significant inhibition of pRB phosphorylation and increase of Cdk inhibitor p21 that appear to be responsible for the observed G1 arrest.

Authors+Show Affiliations

Department of Biochemistry, College of Oriental Medicine, Dong-Eui University, Pusan 614-054, Korea. choiyh@hyomin.dongeui.ac.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11496322

Citation

Choi, Y H., et al. "Doenjang Hexane Fraction-induced G1 Arrest Is Associated With the Inhibition of pRB Phosphorylation and Induction of Cdk Inhibitor P21 in Human Breast Carcinoma MCF-7 Cells." Oncology Reports, vol. 8, no. 5, 2001, pp. 1091-6.
Choi YH, Choi BT, Lee WH, et al. Doenjang hexane fraction-induced G1 arrest is associated with the inhibition of pRB phosphorylation and induction of Cdk inhibitor p21 in human breast carcinoma MCF-7 cells. Oncol Rep. 2001;8(5):1091-6.
Choi, Y. H., Choi, B. T., Lee, W. H., Rhee, S. H., & Park, K. Y. (2001). Doenjang hexane fraction-induced G1 arrest is associated with the inhibition of pRB phosphorylation and induction of Cdk inhibitor p21 in human breast carcinoma MCF-7 cells. Oncology Reports, 8(5), 1091-6.
Choi YH, et al. Doenjang Hexane Fraction-induced G1 Arrest Is Associated With the Inhibition of pRB Phosphorylation and Induction of Cdk Inhibitor P21 in Human Breast Carcinoma MCF-7 Cells. Oncol Rep. 2001 Sep-Oct;8(5):1091-6. PubMed PMID: 11496322.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Doenjang hexane fraction-induced G1 arrest is associated with the inhibition of pRB phosphorylation and induction of Cdk inhibitor p21 in human breast carcinoma MCF-7 cells. AU - Choi,Y H, AU - Choi,B T, AU - Lee,W H, AU - Rhee,S H, AU - Park,K Y, PY - 2001/8/10/pubmed PY - 2001/10/26/medline PY - 2001/8/10/entrez SP - 1091 EP - 6 JF - Oncology reports JO - Oncol Rep VL - 8 IS - 5 N2 - Doenjang (fermented soy paste) is a Korean traditional fermented food. In previous studies, we demonstrated that Doenjang extracts exhibited anti-mutagenic and anti-carcinogenic activities. Here we investigated the effects of Doenjang hexane fraction (DHF) on cell cycle progression in the human breast carcinoma MCF-7 cells. Treatment of DHF to MCF-7 cells induced a G1 phase arrest of the cell cycle, which correlated with the accumulation of the hypophosphorylated form of the retinoblastoma protein (pRB) and enhanced association of pRB with the transcription factor E2F-1. After DHF treatment, the expression of D-type cyclins was decreased in a time-dependent manner, but DHF did not affect the levels of cyclin-dependent kinases (Cdks), cyclin E and cyclin A protein. However, the activity of Cdk2 and cyclin E-associated kinase was decreased in a time-dependent manner. The tumor suppressor p53 and Cdk inhibitor p21, a known downstream effector of the p53, and association of p21 with Cdk2 were markedly induced in DHF-treated cells. Taken together, the present results indicate that treatment of MCF-7 cells with DHF induces a significant inhibition of pRB phosphorylation and increase of Cdk inhibitor p21 that appear to be responsible for the observed G1 arrest. SN - 1021-335X UR - https://www.unboundmedicine.com/medline/citation/11496322/Doenjang_hexane_fraction_induced_G1_arrest_is_associated_with_the_inhibition_of_pRB_phosphorylation_and_induction_of_Cdk_inhibitor_p21_in_human_breast_carcinoma_MCF_7_cells_ L2 - http://www.spandidos-publications.com/or/8/5/1091 DB - PRIME DP - Unbound Medicine ER -