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Global regulators ExpA (GacA) and KdgR modulate extracellular enzyme gene expression through the RsmA-rsmB system in Erwinia carotovora subsp. carotovora.
Mol Plant Microbe Interact. 2001 Aug; 14(8):931-8.MP

Abstract

The production of the main virulence determinants, the extracellular plant cell wall-degrading enzymes, and hence virulence of Erwinia carotovora subsp. carotovora is controlled by a complex regulatory network. One of the global regulators, the response regulator ExpA, a GacA homolog, is required for transcriptional activation of the extracellular enzyme genes of this soft-rot pathogen. To elucidate the mechanism of ExpA control as well as interactions with other regulatory systems, we isolated second-site transposon mutants that would suppress the enzyme-negative phenotype of an expA (gacA) mutant. Inactivation of kdgR resulted in partial restoration of extracellular enzyme production and virulence to the expA mutant, suggesting an interaction between the two regulatory pathways. This interaction was mediated by the RsmA-rsmB system. Northern analysis was used to show that the regulatory rsmB RNA was under positive control of ExpA. Conversely, the expression of rsmA encoding a global repressor was under negative control of ExpA and positive control of KdgR. This study indicates a central role for the RsmA-rsmB regulatory system during pathogenesis, integrating signals from the ExpA (GacA) and KdgR global regulators of extracellular enzyme production in E. carotovora subsp. carotovora.

Authors+Show Affiliations

Department of Biosciences, University of Helsinki, Finland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11497464

Citation

Hyytiäinen, H, et al. "Global Regulators ExpA (GacA) and KdgR Modulate Extracellular Enzyme Gene Expression Through the RsmA-rsmB System in Erwinia Carotovora Subsp. Carotovora." Molecular Plant-microbe Interactions : MPMI, vol. 14, no. 8, 2001, pp. 931-8.
Hyytiäinen H, Montesano M, Palva ET. Global regulators ExpA (GacA) and KdgR modulate extracellular enzyme gene expression through the RsmA-rsmB system in Erwinia carotovora subsp. carotovora. Mol Plant Microbe Interact. 2001;14(8):931-8.
Hyytiäinen, H., Montesano, M., & Palva, E. T. (2001). Global regulators ExpA (GacA) and KdgR modulate extracellular enzyme gene expression through the RsmA-rsmB system in Erwinia carotovora subsp. carotovora. Molecular Plant-microbe Interactions : MPMI, 14(8), 931-8.
Hyytiäinen H, Montesano M, Palva ET. Global Regulators ExpA (GacA) and KdgR Modulate Extracellular Enzyme Gene Expression Through the RsmA-rsmB System in Erwinia Carotovora Subsp. Carotovora. Mol Plant Microbe Interact. 2001;14(8):931-8. PubMed PMID: 11497464.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Global regulators ExpA (GacA) and KdgR modulate extracellular enzyme gene expression through the RsmA-rsmB system in Erwinia carotovora subsp. carotovora. AU - Hyytiäinen,H, AU - Montesano,M, AU - Palva,E T, PY - 2001/8/11/pubmed PY - 2002/1/5/medline PY - 2001/8/11/entrez SP - 931 EP - 8 JF - Molecular plant-microbe interactions : MPMI JO - Mol Plant Microbe Interact VL - 14 IS - 8 N2 - The production of the main virulence determinants, the extracellular plant cell wall-degrading enzymes, and hence virulence of Erwinia carotovora subsp. carotovora is controlled by a complex regulatory network. One of the global regulators, the response regulator ExpA, a GacA homolog, is required for transcriptional activation of the extracellular enzyme genes of this soft-rot pathogen. To elucidate the mechanism of ExpA control as well as interactions with other regulatory systems, we isolated second-site transposon mutants that would suppress the enzyme-negative phenotype of an expA (gacA) mutant. Inactivation of kdgR resulted in partial restoration of extracellular enzyme production and virulence to the expA mutant, suggesting an interaction between the two regulatory pathways. This interaction was mediated by the RsmA-rsmB system. Northern analysis was used to show that the regulatory rsmB RNA was under positive control of ExpA. Conversely, the expression of rsmA encoding a global repressor was under negative control of ExpA and positive control of KdgR. This study indicates a central role for the RsmA-rsmB regulatory system during pathogenesis, integrating signals from the ExpA (GacA) and KdgR global regulators of extracellular enzyme production in E. carotovora subsp. carotovora. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/11497464/Global_regulators_ExpA__GacA__and_KdgR_modulate_extracellular_enzyme_gene_expression_through_the_RsmA_rsmB_system_in_Erwinia_carotovora_subsp__carotovora_ L2 - https://apsjournals.apsnet.org/doi/abs/10.1094/MPMI.2001.14.8.931?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -