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Effect of melittin on potassium currents and action potential in ventricular myocytes of guinea pig.
Acta Pharmacol Sin. 2000 Sep; 21(9):841-4.AP

Abstract

AIM

To examine the effects of melittin (Mel), the major component of bee venom, on delayed rectifier K+ current (Ik), inward rectifier K+ current (Ikl) and action potential (AP) in guinea pig ventricular myocytes.

METHODS

Ik, Ikl, and AP were recorded using the whole-cell patch-clamp technique.

RESULTS

The action potential duration (APD) was shortened by Mel in a concentration-dependent manner. Mel 0.05, 0.1, 0.2 mumol.L-1 shortened APD50 from (520 +/- 55) to (459 +/- 91) (n = 5, P > 0.05), (385 +/- 102) (n = 5, P < 0.01), and (281 +/- 81) ms (n = 5, P < 0.01), respectively; and APD90 from (613 +/- 96) to (536 +/- 93) (n = 5, P > 0.05), (467 +/- 96) ms (n = 5, P < 0.01), and (354 +/- 95) ms (n = 5, P < 0.01), respectively. Mel increased the amplitude of Ik also in a concentration-dependent manner. Mel 0.05, 0.1, 0.2 mumol.L-1 increased Ik from (295 +/- 109) to (371 +/- 142) (n = 5, P < 0.05), (467 +/- 180) (n = 5, P < 0.05), (552 +/- 248) pA (n = 5, P < 0.05), respectively at testing potential of + 40 mV. But no significant effect of Mel on Ikl was observed at these concentrations.

CONCLUSION

Mel significantly increased Ik in a concentration-dependent manner which contributed to shortening of APD.

Authors+Show Affiliations

Department of Pharmacology, School of Pharmacy, State Key Laboratory of Medical Neurobiology, Shanghai Medical University, Shanghai 200032, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11501168

Citation

Zhang, X M., et al. "Effect of Melittin On Potassium Currents and Action Potential in Ventricular Myocytes of Guinea Pig." Acta Pharmacologica Sinica, vol. 21, no. 9, 2000, pp. 841-4.
Zhang XM, Yang S, He XJ, et al. Effect of melittin on potassium currents and action potential in ventricular myocytes of guinea pig. Acta Pharmacol Sin. 2000;21(9):841-4.
Zhang, X. M., Yang, S., He, X. J., Zheng, P., & Jiang, M. H. (2000). Effect of melittin on potassium currents and action potential in ventricular myocytes of guinea pig. Acta Pharmacologica Sinica, 21(9), 841-4.
Zhang XM, et al. Effect of Melittin On Potassium Currents and Action Potential in Ventricular Myocytes of Guinea Pig. Acta Pharmacol Sin. 2000;21(9):841-4. PubMed PMID: 11501168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of melittin on potassium currents and action potential in ventricular myocytes of guinea pig. AU - Zhang,X M, AU - Yang,S, AU - He,X J, AU - Zheng,P, AU - Jiang,M H, PY - 2001/8/15/pubmed PY - 2001/10/19/medline PY - 2001/8/15/entrez SP - 841 EP - 4 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 21 IS - 9 N2 - AIM: To examine the effects of melittin (Mel), the major component of bee venom, on delayed rectifier K+ current (Ik), inward rectifier K+ current (Ikl) and action potential (AP) in guinea pig ventricular myocytes. METHODS: Ik, Ikl, and AP were recorded using the whole-cell patch-clamp technique. RESULTS: The action potential duration (APD) was shortened by Mel in a concentration-dependent manner. Mel 0.05, 0.1, 0.2 mumol.L-1 shortened APD50 from (520 +/- 55) to (459 +/- 91) (n = 5, P > 0.05), (385 +/- 102) (n = 5, P < 0.01), and (281 +/- 81) ms (n = 5, P < 0.01), respectively; and APD90 from (613 +/- 96) to (536 +/- 93) (n = 5, P > 0.05), (467 +/- 96) ms (n = 5, P < 0.01), and (354 +/- 95) ms (n = 5, P < 0.01), respectively. Mel increased the amplitude of Ik also in a concentration-dependent manner. Mel 0.05, 0.1, 0.2 mumol.L-1 increased Ik from (295 +/- 109) to (371 +/- 142) (n = 5, P < 0.05), (467 +/- 180) (n = 5, P < 0.05), (552 +/- 248) pA (n = 5, P < 0.05), respectively at testing potential of + 40 mV. But no significant effect of Mel on Ikl was observed at these concentrations. CONCLUSION: Mel significantly increased Ik in a concentration-dependent manner which contributed to shortening of APD. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/11501168/Effect_of_melittin_on_potassium_currents_and_action_potential_in_ventricular_myocytes_of_guinea_pig_ L2 - http://www.chinaphar.com/1671-4083/21/841.pdf DB - PRIME DP - Unbound Medicine ER -