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Gene and protein expressions of nitric oxide synthases in ischemia-reperfused peripheral nerve of the rat.
Am J Physiol Cell Physiol. 2001 Sep; 281(3):C849-56.AJ

Abstract

This study examined mRNA and protein expressions of neuronal (nNOS), inducible (iNOS), and endothelial nitric oxide synthases (eNOS) in peripheral nerve after ischemia-reperfusion (I/R). Sixty-six rats were divided into the ischemia only and I/R groups. One sciatic nerve of each animal was used as the experimental side and the opposite untreated nerve as the control. mRNA levels in the nerve were quantitatively measured by competitive PCR, and protein was determined by Western blotting and immunohistochemical staining. The results showed that, after ischemia (2 h), both nNOS and eNOS protein expressions decreased. After I/R (2 h of ischemia followed by 3 h of reperfusion), expression of both nNOS and eNOS mRNA and protein decreased further. In contrast, iNOS mRNA significantly increased after ischemia and was further upregulated (14-fold) after I/R, while iNOS protein was not detected. The results reveal the dynamic expression of individual NOS isoforms during the course of I/R injury. An understanding of this modulation on a cellular and molecular level may lead to understanding the mechanisms of I/R injury and to methods of ameliorating peripheral nerve injury.

Authors+Show Affiliations

Orthopaedic Cell Biology Laboratory, Duke University Medical Center, Durham, North Carolina 27710, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11502562

Citation

Qi, W N., et al. "Gene and Protein Expressions of Nitric Oxide Synthases in Ischemia-reperfused Peripheral Nerve of the Rat." American Journal of Physiology. Cell Physiology, vol. 281, no. 3, 2001, pp. C849-56.
Qi WN, Yan ZQ, Whang PG, et al. Gene and protein expressions of nitric oxide synthases in ischemia-reperfused peripheral nerve of the rat. Am J Physiol Cell Physiol. 2001;281(3):C849-56.
Qi, W. N., Yan, Z. Q., Whang, P. G., Zhou, Q., Chen, L. E., Seaber, A. V., Stamler, J. S., & Urbaniak, J. R. (2001). Gene and protein expressions of nitric oxide synthases in ischemia-reperfused peripheral nerve of the rat. American Journal of Physiology. Cell Physiology, 281(3), C849-56.
Qi WN, et al. Gene and Protein Expressions of Nitric Oxide Synthases in Ischemia-reperfused Peripheral Nerve of the Rat. Am J Physiol Cell Physiol. 2001;281(3):C849-56. PubMed PMID: 11502562.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene and protein expressions of nitric oxide synthases in ischemia-reperfused peripheral nerve of the rat. AU - Qi,W N, AU - Yan,Z Q, AU - Whang,P G, AU - Zhou,Q, AU - Chen,L E, AU - Seaber,A V, AU - Stamler,J S, AU - Urbaniak,J R, PY - 2001/8/15/pubmed PY - 2001/9/14/medline PY - 2001/8/15/entrez SP - C849 EP - 56 JF - American journal of physiology. Cell physiology JO - Am J Physiol Cell Physiol VL - 281 IS - 3 N2 - This study examined mRNA and protein expressions of neuronal (nNOS), inducible (iNOS), and endothelial nitric oxide synthases (eNOS) in peripheral nerve after ischemia-reperfusion (I/R). Sixty-six rats were divided into the ischemia only and I/R groups. One sciatic nerve of each animal was used as the experimental side and the opposite untreated nerve as the control. mRNA levels in the nerve were quantitatively measured by competitive PCR, and protein was determined by Western blotting and immunohistochemical staining. The results showed that, after ischemia (2 h), both nNOS and eNOS protein expressions decreased. After I/R (2 h of ischemia followed by 3 h of reperfusion), expression of both nNOS and eNOS mRNA and protein decreased further. In contrast, iNOS mRNA significantly increased after ischemia and was further upregulated (14-fold) after I/R, while iNOS protein was not detected. The results reveal the dynamic expression of individual NOS isoforms during the course of I/R injury. An understanding of this modulation on a cellular and molecular level may lead to understanding the mechanisms of I/R injury and to methods of ameliorating peripheral nerve injury. SN - 0363-6143 UR - https://www.unboundmedicine.com/medline/citation/11502562/Gene_and_protein_expressions_of_nitric_oxide_synthases_in_ischemia_reperfused_peripheral_nerve_of_the_rat_ L2 - https://journals.physiology.org/doi/10.1152/ajpcell.2001.281.3.C849?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -