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Spatially focused deposition of capillary electrophoresis effluent onto surface-enhanced Raman-active substrates for off-column spectroscopy.
Electrophoresis. 2001 Jul; 22(11):2303-11.E

Abstract

Surface-enhanced Raman scattering (SERS) is employed to obtain distinctive spectra of compounds that are efficiently separated by capillary electrophoresis (CE) and deposited onto planar SERS-active substrates. A simple method is described that explains how to prepare SERS-active substrates by depositing a silver-colloid solution onto frosted-glass microscope slides, using a high-efficiency nebulizer. Scanning electron micrographs reveal a layered coating of fairly uniform-sized, 100-nm silver nanoparticles with interstitial spaces ranging from a few to tens of nanometers. The on-column separation is monitored by laser-induced fluorescence, while electrofilament depositing the CE effluent onto a moving SERS-substrate. Subsequently, the SERS spectra and off-column electropherograms are obtained with a simple confocal Raman spectrometer. The test compounds used to demonstrate this technique include compounds of biological significance: benzyloxyresorufin, riboflavin, and resorufin. CE and Raman conditions are evaluated to determine their affects on the SERS signals. An average off-column efficiency of 100,000 plates/m and a signal reproducibility of 11% relative standard deviation were achieved. Characteristic spectra with major Raman bands exhibiting signal-to-noise ratios of greater than 3 were obtained for a 3.2-nL injection of 10(-6) M (706 fg) resorufin. Forming a self-assembled monolayer (SAM) on the substrate increases the sensitivity of the SERS technique and decreases the on-substrate broadening. Calibration plots for both plain- and SAM-SERS substrates are demonstrated.

Authors+Show Affiliations

Department of Chemistry, University of Tennessee, Knoxville 37996-1600, USA.No affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

11504066

Citation

DeVault, G L., and M J. Sepaniak. "Spatially Focused Deposition of Capillary Electrophoresis Effluent Onto Surface-enhanced Raman-active Substrates for Off-column Spectroscopy." Electrophoresis, vol. 22, no. 11, 2001, pp. 2303-11.
DeVault GL, Sepaniak MJ. Spatially focused deposition of capillary electrophoresis effluent onto surface-enhanced Raman-active substrates for off-column spectroscopy. Electrophoresis. 2001;22(11):2303-11.
DeVault, G. L., & Sepaniak, M. J. (2001). Spatially focused deposition of capillary electrophoresis effluent onto surface-enhanced Raman-active substrates for off-column spectroscopy. Electrophoresis, 22(11), 2303-11.
DeVault GL, Sepaniak MJ. Spatially Focused Deposition of Capillary Electrophoresis Effluent Onto Surface-enhanced Raman-active Substrates for Off-column Spectroscopy. Electrophoresis. 2001;22(11):2303-11. PubMed PMID: 11504066.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spatially focused deposition of capillary electrophoresis effluent onto surface-enhanced Raman-active substrates for off-column spectroscopy. AU - DeVault,G L, AU - Sepaniak,M J, PY - 2001/8/16/pubmed PY - 2002/2/1/medline PY - 2001/8/16/entrez SP - 2303 EP - 11 JF - Electrophoresis JO - Electrophoresis VL - 22 IS - 11 N2 - Surface-enhanced Raman scattering (SERS) is employed to obtain distinctive spectra of compounds that are efficiently separated by capillary electrophoresis (CE) and deposited onto planar SERS-active substrates. A simple method is described that explains how to prepare SERS-active substrates by depositing a silver-colloid solution onto frosted-glass microscope slides, using a high-efficiency nebulizer. Scanning electron micrographs reveal a layered coating of fairly uniform-sized, 100-nm silver nanoparticles with interstitial spaces ranging from a few to tens of nanometers. The on-column separation is monitored by laser-induced fluorescence, while electrofilament depositing the CE effluent onto a moving SERS-substrate. Subsequently, the SERS spectra and off-column electropherograms are obtained with a simple confocal Raman spectrometer. The test compounds used to demonstrate this technique include compounds of biological significance: benzyloxyresorufin, riboflavin, and resorufin. CE and Raman conditions are evaluated to determine their affects on the SERS signals. An average off-column efficiency of 100,000 plates/m and a signal reproducibility of 11% relative standard deviation were achieved. Characteristic spectra with major Raman bands exhibiting signal-to-noise ratios of greater than 3 were obtained for a 3.2-nL injection of 10(-6) M (706 fg) resorufin. Forming a self-assembled monolayer (SAM) on the substrate increases the sensitivity of the SERS technique and decreases the on-substrate broadening. Calibration plots for both plain- and SAM-SERS substrates are demonstrated. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/11504066/Spatially_focused_deposition_of_capillary_electrophoresis_effluent_onto_surface_enhanced_Raman_active_substrates_for_off_column_spectroscopy_ L2 - https://doi.org/10.1002/1522-2683(20017)22:11<2303::AID-ELPS2303>3.0.CO;2-A DB - PRIME DP - Unbound Medicine ER -