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Electrically driven microseparation methods for pesticides and metabolites: VI. Surfactant-mediated electrokinetic capillary chromatography of aniline pesticidic metabolites derivatized with 9-fluoroenylmethyl chloroformate and their detection by laser-induced fluorescence.
Electrophoresis. 2001 Jul; 22(11):2320-6.E

Abstract

In this report, we describe a surfactant-mediated electrokinetic capillary chromatography (SM-EKC) system for the separation of 9-fluoroenylmethyl chloroformate (FMOC)-derivatized anilines by capillary electrophoresis (CE). The SM-EKC system consisted of dioctyl sulfosuccinate (DOSS)/acetonitrile mixtures and was suited for the CE separation of the relatively hydrophobic FMOC-aniline analytes and other neutral compounds, e.g. alkylphenyl ketones. While the organic modifier acetonitrile (ACN) allowed the solubilization of the hydrophobic solutes and maintained the DOSS surfactant in its monomeric form by inhibiting micellization, the DOSS surfactant associated with the FMOC anilines to a varying degree thus leading to their differential migration and separation. Under these conditions, the FMOC-anilines were readily detected at the 10(-6) M level by UV at 214 nm and at the 10(-8) M level by laser-induced fluorescence (LIF) using a solid-state UV laser operating at 266 nm line as the excitation wavelength. The FMOC precolumn derivatization was also readily performed in lake water spiked with anilines at near the limit of detection (LOD) level. The lake water matrix showed no significant effects on the extent of derivatization at the LOD level as well as on the detection of the analytes due to the selectivity of the FMOC derivatization. The derivatization and detection of spiked lake water necessitated only the removal of microparticles by microfiltration prior to derivatization and detection.

Authors+Show Affiliations

Department of Chemistry, Oklahoma State University, Stillwater 74078-3071, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

11504068

Citation

Wall, W, et al. "Electrically Driven Microseparation Methods for Pesticides and Metabolites: VI. Surfactant-mediated Electrokinetic Capillary Chromatography of Aniline Pesticidic Metabolites Derivatized With 9-fluoroenylmethyl Chloroformate and Their Detection By Laser-induced Fluorescence." Electrophoresis, vol. 22, no. 11, 2001, pp. 2320-6.
Wall W, Chan K, El Rassi Z. Electrically driven microseparation methods for pesticides and metabolites: VI. Surfactant-mediated electrokinetic capillary chromatography of aniline pesticidic metabolites derivatized with 9-fluoroenylmethyl chloroformate and their detection by laser-induced fluorescence. Electrophoresis. 2001;22(11):2320-6.
Wall, W., Chan, K., & El Rassi, Z. (2001). Electrically driven microseparation methods for pesticides and metabolites: VI. Surfactant-mediated electrokinetic capillary chromatography of aniline pesticidic metabolites derivatized with 9-fluoroenylmethyl chloroformate and their detection by laser-induced fluorescence. Electrophoresis, 22(11), 2320-6.
Wall W, Chan K, El Rassi Z. Electrically Driven Microseparation Methods for Pesticides and Metabolites: VI. Surfactant-mediated Electrokinetic Capillary Chromatography of Aniline Pesticidic Metabolites Derivatized With 9-fluoroenylmethyl Chloroformate and Their Detection By Laser-induced Fluorescence. Electrophoresis. 2001;22(11):2320-6. PubMed PMID: 11504068.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrically driven microseparation methods for pesticides and metabolites: VI. Surfactant-mediated electrokinetic capillary chromatography of aniline pesticidic metabolites derivatized with 9-fluoroenylmethyl chloroformate and their detection by laser-induced fluorescence. AU - Wall,W, AU - Chan,K, AU - El Rassi,Z, PY - 2001/8/16/pubmed PY - 2002/2/1/medline PY - 2001/8/16/entrez SP - 2320 EP - 6 JF - Electrophoresis JO - Electrophoresis VL - 22 IS - 11 N2 - In this report, we describe a surfactant-mediated electrokinetic capillary chromatography (SM-EKC) system for the separation of 9-fluoroenylmethyl chloroformate (FMOC)-derivatized anilines by capillary electrophoresis (CE). The SM-EKC system consisted of dioctyl sulfosuccinate (DOSS)/acetonitrile mixtures and was suited for the CE separation of the relatively hydrophobic FMOC-aniline analytes and other neutral compounds, e.g. alkylphenyl ketones. While the organic modifier acetonitrile (ACN) allowed the solubilization of the hydrophobic solutes and maintained the DOSS surfactant in its monomeric form by inhibiting micellization, the DOSS surfactant associated with the FMOC anilines to a varying degree thus leading to their differential migration and separation. Under these conditions, the FMOC-anilines were readily detected at the 10(-6) M level by UV at 214 nm and at the 10(-8) M level by laser-induced fluorescence (LIF) using a solid-state UV laser operating at 266 nm line as the excitation wavelength. The FMOC precolumn derivatization was also readily performed in lake water spiked with anilines at near the limit of detection (LOD) level. The lake water matrix showed no significant effects on the extent of derivatization at the LOD level as well as on the detection of the analytes due to the selectivity of the FMOC derivatization. The derivatization and detection of spiked lake water necessitated only the removal of microparticles by microfiltration prior to derivatization and detection. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/11504068/Electrically_driven_microseparation_methods_for_pesticides_and_metabolites:_VI__Surfactant_mediated_electrokinetic_capillary_chromatography_of_aniline_pesticidic_metabolites_derivatized_with_9_fluoroenylmethyl_chloroformate_and_their_detection_by_laser_induced_fluorescence_ L2 - https://doi.org/10.1002/1522-2683(20017)22:11<2320::AID-ELPS2320>3.0.CO;2-O DB - PRIME DP - Unbound Medicine ER -