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Determination of phycobiliproteins by capillary electrophoresis with laser-induced fluorescence detection.
Electrophoresis. 2001 Jul; 22(11):2327-35.E

Abstract

Phycobiliproteins are derived from the photosynthetic apparatus of cyanobacteria and eukaryotic algae. They are composed of a protein backbone to which linear tetrapyrrole chromophores are covalently bound. Furthermore, they are water-soluble highly fluorescent, and relatively stable at room temperature and neutral pH. For this reason, capillary electrophoresis-laser induced fluorescence (CE-LIF) seems the idea method for determination of these important proteins. The effects of buffer additives such as sodium dodecyl sulfate (SDS)and putrescine on the separation of the three major phycobiliprotein types, namely allophycocyanin, phycocyanin, and phycoerythrin, with excitation and emission maxima at 652/660, 615/647, and 565(494)/575 nm, respectively, are considered. Detection limits for these proteins by CE-LIF are some 60-500 times better than by absorbance detection. The development of a fast and sensitive CE-LIF assay such as this is of potential significance to our understand ing of chemical and biological oceanographic processes.

Authors+Show Affiliations

Wake Forest University, Winston-Salem, NC 27109, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

11504069

Citation

Viskari, P J., et al. "Determination of Phycobiliproteins By Capillary Electrophoresis With Laser-induced Fluorescence Detection." Electrophoresis, vol. 22, no. 11, 2001, pp. 2327-35.
Viskari PJ, Kinkade CS, Colyer CL. Determination of phycobiliproteins by capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis. 2001;22(11):2327-35.
Viskari, P. J., Kinkade, C. S., & Colyer, C. L. (2001). Determination of phycobiliproteins by capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis, 22(11), 2327-35.
Viskari PJ, Kinkade CS, Colyer CL. Determination of Phycobiliproteins By Capillary Electrophoresis With Laser-induced Fluorescence Detection. Electrophoresis. 2001;22(11):2327-35. PubMed PMID: 11504069.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of phycobiliproteins by capillary electrophoresis with laser-induced fluorescence detection. AU - Viskari,P J, AU - Kinkade,C S, AU - Colyer,C L, PY - 2001/8/16/pubmed PY - 2002/2/1/medline PY - 2001/8/16/entrez SP - 2327 EP - 35 JF - Electrophoresis JO - Electrophoresis VL - 22 IS - 11 N2 - Phycobiliproteins are derived from the photosynthetic apparatus of cyanobacteria and eukaryotic algae. They are composed of a protein backbone to which linear tetrapyrrole chromophores are covalently bound. Furthermore, they are water-soluble highly fluorescent, and relatively stable at room temperature and neutral pH. For this reason, capillary electrophoresis-laser induced fluorescence (CE-LIF) seems the idea method for determination of these important proteins. The effects of buffer additives such as sodium dodecyl sulfate (SDS)and putrescine on the separation of the three major phycobiliprotein types, namely allophycocyanin, phycocyanin, and phycoerythrin, with excitation and emission maxima at 652/660, 615/647, and 565(494)/575 nm, respectively, are considered. Detection limits for these proteins by CE-LIF are some 60-500 times better than by absorbance detection. The development of a fast and sensitive CE-LIF assay such as this is of potential significance to our understand ing of chemical and biological oceanographic processes. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/11504069/Determination_of_phycobiliproteins_by_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ L2 - https://doi.org/10.1002/1522-2683(20017)22:11<2327::AID-ELPS2327>3.0.CO;2-J DB - PRIME DP - Unbound Medicine ER -