Tags

Type your tag names separated by a space and hit enter

Chromosome mapping and phylogenetic analysis of the cytosolic acetyl-CoA carboxylase loci in wheat.
Mol Biol Evol. 2001 Sep; 18(9):1720-33.MB

Abstract

The cytosolic isoform of plant acetyl-CoA carboxylase is a multidomain enzyme involved in the synthesis of very-long-chain fatty acids and in secondary metabolism. Chromosome mapping of wheat identified one locus containing cytosolic acetyl-CoA carboxylase genes (Acc-2) and a related partially processed pseudogene (Psi-Acc-2) in the distal region of the long arm of wheat homoeologous group 3 chromosomes. Multiple copies of the Acc-2 genes, whose presence was suggested by sequence analysis, are likely to be arranged in tandem repeats. At least three out of five genes cloned from hexaploid wheat map to this locus. Another locus containing Acc-2--related sequences is present in the distal region of the long arm of chromosome 5D. The identity of the hybridizing DNA present at this locus remains unknown. A system based on PCR-cloning and DNA sequence analysis of acetyl-CoA carboxylase genes was developed to address various phylogenetic and systematics questions in grasses. It was applied to reconstruct the phylogeny of the Acc-2 genes from D- and S-genome Aegilops and A-genome Triticum diploid species, AABB- and AAGG-genome tetraploid wheat, and AABBDD-genome hexaploid wheat, as well as from rye and barley. The combined cytogenetic and molecular evolution approach allowed assignment of gene sequences included in phylogenetic analysis to specific loci on homoeologous chromosomes. Recurring gene duplication followed by chromosome translocation and/or possible loss of some gene copies, as well as loss of introns, occurred in the gene family in different plant lineages. Two major Acc-2 clades appeared before the divergence of barley and rye. Nucleotide substitution rates in different parts of the Acc-2 gene were assessed. This analysis of the Acc-2 loci provides detailed information regarding evolutionary events at a low--copy-number locus containing important functional genes. These events are likely to be common and to play a significant role in shaping grass genomes.

Authors+Show Affiliations

Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11504852

Citation

Faris, J, et al. "Chromosome Mapping and Phylogenetic Analysis of the Cytosolic acetyl-CoA Carboxylase Loci in Wheat." Molecular Biology and Evolution, vol. 18, no. 9, 2001, pp. 1720-33.
Faris J, Sirikhachornkit A, Haselkorn R, et al. Chromosome mapping and phylogenetic analysis of the cytosolic acetyl-CoA carboxylase loci in wheat. Mol Biol Evol. 2001;18(9):1720-33.
Faris, J., Sirikhachornkit, A., Haselkorn, R., Gill, B., & Gornicki, P. (2001). Chromosome mapping and phylogenetic analysis of the cytosolic acetyl-CoA carboxylase loci in wheat. Molecular Biology and Evolution, 18(9), 1720-33.
Faris J, et al. Chromosome Mapping and Phylogenetic Analysis of the Cytosolic acetyl-CoA Carboxylase Loci in Wheat. Mol Biol Evol. 2001;18(9):1720-33. PubMed PMID: 11504852.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chromosome mapping and phylogenetic analysis of the cytosolic acetyl-CoA carboxylase loci in wheat. AU - Faris,J, AU - Sirikhachornkit,A, AU - Haselkorn,R, AU - Gill,B, AU - Gornicki,P, PY - 2001/8/16/pubmed PY - 2001/10/5/medline PY - 2001/8/16/entrez SP - 1720 EP - 33 JF - Molecular biology and evolution JO - Mol. Biol. Evol. VL - 18 IS - 9 N2 - The cytosolic isoform of plant acetyl-CoA carboxylase is a multidomain enzyme involved in the synthesis of very-long-chain fatty acids and in secondary metabolism. Chromosome mapping of wheat identified one locus containing cytosolic acetyl-CoA carboxylase genes (Acc-2) and a related partially processed pseudogene (Psi-Acc-2) in the distal region of the long arm of wheat homoeologous group 3 chromosomes. Multiple copies of the Acc-2 genes, whose presence was suggested by sequence analysis, are likely to be arranged in tandem repeats. At least three out of five genes cloned from hexaploid wheat map to this locus. Another locus containing Acc-2--related sequences is present in the distal region of the long arm of chromosome 5D. The identity of the hybridizing DNA present at this locus remains unknown. A system based on PCR-cloning and DNA sequence analysis of acetyl-CoA carboxylase genes was developed to address various phylogenetic and systematics questions in grasses. It was applied to reconstruct the phylogeny of the Acc-2 genes from D- and S-genome Aegilops and A-genome Triticum diploid species, AABB- and AAGG-genome tetraploid wheat, and AABBDD-genome hexaploid wheat, as well as from rye and barley. The combined cytogenetic and molecular evolution approach allowed assignment of gene sequences included in phylogenetic analysis to specific loci on homoeologous chromosomes. Recurring gene duplication followed by chromosome translocation and/or possible loss of some gene copies, as well as loss of introns, occurred in the gene family in different plant lineages. Two major Acc-2 clades appeared before the divergence of barley and rye. Nucleotide substitution rates in different parts of the Acc-2 gene were assessed. This analysis of the Acc-2 loci provides detailed information regarding evolutionary events at a low--copy-number locus containing important functional genes. These events are likely to be common and to play a significant role in shaping grass genomes. SN - 0737-4038 UR - https://www.unboundmedicine.com/medline/citation/11504852/Chromosome_mapping_and_phylogenetic_analysis_of_the_cytosolic_acetyl_CoA_carboxylase_loci_in_wheat_ L2 - https://academic.oup.com/mbe/article-lookup/doi/10.1093/oxfordjournals.molbev.a003960 DB - PRIME DP - Unbound Medicine ER -