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Venom from Anemesia species of spider modulates high voltage-activated Ca(2+) currents from rat cultured sensory neurones and excitatory post synaptic currents from rat hippocampal slices.
Cell Calcium. 2001 Sep; 30(3):212-21.CC

Abstract

The actions of crude venom from Anemesia species of spider were investigated in cultured dorsal root ganglion neurones from neonatal rats and hippocampal slices. Using mass spectrometry (MALDI-TOF MS), 10-12 distinct peptides with masses between about 3 and 10kDa were identified in the crude spider venom. At a concentration of 5 microg/ml crude Anemesia venom transiently enhanced the mean peak whole cell voltage-activated Ca(2+) current in a voltage-dependent manner and potentiated transient increases in intracellular Ca(2+) triggered by 30mM KCI as measured using Fura-2 fluorescence imaging. Additionally, 5-8 microg/ml Anemesia venom increased the amplitude of glutamatergic excitatory postsynaptic currents evoked in hippocampal slices. Omega-Conotoxin GVIA (1 microM) prevented the increase in voltage-activated Ca(2+) currents produced by Anemesia venom. This attenuation occurred when the cone shell toxin was applied before or after the spider venom. Anemesia venom (5 microg/ml) created no significant change in evoked action potentials but produced modest but significant inhibition of voltage-activated K(+) currents. At a concentration of 50 microg/ml Anemesia venom only produced reversible inhibitory effects, decreasing voltage-activated Ca(2+) currents. However, no significant effects on Ca(2+) currents were observed with a concentration of 0.5 microg/ml. The toxin(s) in the venom that enhanced Ca(2+) influx into sensory neurones was heat-sensitive and was made inactive by boiling or repetitive freeze-thawing. Boiled venom (5 microg/ml) produced significant inhibition of voltage-activated Ca(2+) currents and freeze-thawed venom inhibited Ca(2+) transients measured using Fura-2 fluorescence. Our data suggest that crude Anemesia venom contains components, which increased neuronal excitability and neurotransmission, at least in part this was mediated by enhancing Ca(2+) influx through N-type voltage-activated Ca(2+) channels.

Authors+Show Affiliations

Uzbek Academy of Science, Institute of Physiology & Biophysics, Tashkent, Uzbekistan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11509000

Citation

Kalikulov, D, et al. "Venom From Anemesia Species of Spider Modulates High Voltage-activated Ca(2+) Currents From Rat Cultured Sensory Neurones and Excitatory Post Synaptic Currents From Rat Hippocampal Slices." Cell Calcium, vol. 30, no. 3, 2001, pp. 212-21.
Kalikulov D, Ayar A, Nuritova F, et al. Venom from Anemesia species of spider modulates high voltage-activated Ca(2+) currents from rat cultured sensory neurones and excitatory post synaptic currents from rat hippocampal slices. Cell Calcium. 2001;30(3):212-21.
Kalikulov, D., Ayar, A., Nuritova, F., Frenguelli, B. G., McClelland, D., Martin, D. J., Davidson, I., & Scott, R. H. (2001). Venom from Anemesia species of spider modulates high voltage-activated Ca(2+) currents from rat cultured sensory neurones and excitatory post synaptic currents from rat hippocampal slices. Cell Calcium, 30(3), 212-21.
Kalikulov D, et al. Venom From Anemesia Species of Spider Modulates High Voltage-activated Ca(2+) Currents From Rat Cultured Sensory Neurones and Excitatory Post Synaptic Currents From Rat Hippocampal Slices. Cell Calcium. 2001;30(3):212-21. PubMed PMID: 11509000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Venom from Anemesia species of spider modulates high voltage-activated Ca(2+) currents from rat cultured sensory neurones and excitatory post synaptic currents from rat hippocampal slices. AU - Kalikulov,D, AU - Ayar,A, AU - Nuritova,F, AU - Frenguelli,B G, AU - McClelland,D, AU - Martin,D J, AU - Davidson,I, AU - Scott,R H, PY - 2001/8/18/pubmed PY - 2001/10/12/medline PY - 2001/8/18/entrez SP - 212 EP - 21 JF - Cell calcium JO - Cell Calcium VL - 30 IS - 3 N2 - The actions of crude venom from Anemesia species of spider were investigated in cultured dorsal root ganglion neurones from neonatal rats and hippocampal slices. Using mass spectrometry (MALDI-TOF MS), 10-12 distinct peptides with masses between about 3 and 10kDa were identified in the crude spider venom. At a concentration of 5 microg/ml crude Anemesia venom transiently enhanced the mean peak whole cell voltage-activated Ca(2+) current in a voltage-dependent manner and potentiated transient increases in intracellular Ca(2+) triggered by 30mM KCI as measured using Fura-2 fluorescence imaging. Additionally, 5-8 microg/ml Anemesia venom increased the amplitude of glutamatergic excitatory postsynaptic currents evoked in hippocampal slices. Omega-Conotoxin GVIA (1 microM) prevented the increase in voltage-activated Ca(2+) currents produced by Anemesia venom. This attenuation occurred when the cone shell toxin was applied before or after the spider venom. Anemesia venom (5 microg/ml) created no significant change in evoked action potentials but produced modest but significant inhibition of voltage-activated K(+) currents. At a concentration of 50 microg/ml Anemesia venom only produced reversible inhibitory effects, decreasing voltage-activated Ca(2+) currents. However, no significant effects on Ca(2+) currents were observed with a concentration of 0.5 microg/ml. The toxin(s) in the venom that enhanced Ca(2+) influx into sensory neurones was heat-sensitive and was made inactive by boiling or repetitive freeze-thawing. Boiled venom (5 microg/ml) produced significant inhibition of voltage-activated Ca(2+) currents and freeze-thawed venom inhibited Ca(2+) transients measured using Fura-2 fluorescence. Our data suggest that crude Anemesia venom contains components, which increased neuronal excitability and neurotransmission, at least in part this was mediated by enhancing Ca(2+) influx through N-type voltage-activated Ca(2+) channels. SN - 0143-4160 UR - https://www.unboundmedicine.com/medline/citation/11509000/Venom_from_Anemesia_species_of_spider_modulates_high_voltage_activated_Ca_2+__currents_from_rat_cultured_sensory_neurones_and_excitatory_post_synaptic_currents_from_rat_hippocampal_slices_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0143-4160(01)90228-3 DB - PRIME DP - Unbound Medicine ER -