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Replication from oriP of Epstein-Barr virus requires human ORC and is inhibited by geminin.
Cell. 2001 Aug 10; 106(3):287-96.Cell

Abstract

A hypomorphic mutation made in the ORC2 gene of a human cancer cell line through homologous recombination decreased Orc2 protein levels by 90%. The G1 phase of the cell cycle was prolonged, but there was no effect on the utilization of either the c-Myc or beta-globin cellular origins of replication. Cells carrying this mutation failed to support the replication of a plasmid bearing the oriP replicator of Epstein Barr virus (EBV), and this defect was rescued by reintroduction of Orc2. Orc2 specifically associates with oriP in cells, most likely through its interaction with EBNA1. Geminin, an inhibitor of the mammalian replication initiation complex, inhibits replication from oriP. Therefore, ORC and the human replication initiation apparatus is required for replication from a viral origin of replication.

Authors+Show Affiliations

Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11509178

Citation

Dhar, S K., et al. "Replication From oriP of Epstein-Barr Virus Requires Human ORC and Is Inhibited By Geminin." Cell, vol. 106, no. 3, 2001, pp. 287-96.
Dhar SK, Yoshida K, Machida Y, et al. Replication from oriP of Epstein-Barr virus requires human ORC and is inhibited by geminin. Cell. 2001;106(3):287-96.
Dhar, S. K., Yoshida, K., Machida, Y., Khaira, P., Chaudhuri, B., Wohlschlegel, J. A., Leffak, M., Yates, J., & Dutta, A. (2001). Replication from oriP of Epstein-Barr virus requires human ORC and is inhibited by geminin. Cell, 106(3), 287-96.
Dhar SK, et al. Replication From oriP of Epstein-Barr Virus Requires Human ORC and Is Inhibited By Geminin. Cell. 2001 Aug 10;106(3):287-96. PubMed PMID: 11509178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Replication from oriP of Epstein-Barr virus requires human ORC and is inhibited by geminin. AU - Dhar,S K, AU - Yoshida,K, AU - Machida,Y, AU - Khaira,P, AU - Chaudhuri,B, AU - Wohlschlegel,J A, AU - Leffak,M, AU - Yates,J, AU - Dutta,A, PY - 2001/8/18/pubmed PY - 2001/8/31/medline PY - 2001/8/18/entrez SP - 287 EP - 96 JF - Cell JO - Cell VL - 106 IS - 3 N2 - A hypomorphic mutation made in the ORC2 gene of a human cancer cell line through homologous recombination decreased Orc2 protein levels by 90%. The G1 phase of the cell cycle was prolonged, but there was no effect on the utilization of either the c-Myc or beta-globin cellular origins of replication. Cells carrying this mutation failed to support the replication of a plasmid bearing the oriP replicator of Epstein Barr virus (EBV), and this defect was rescued by reintroduction of Orc2. Orc2 specifically associates with oriP in cells, most likely through its interaction with EBNA1. Geminin, an inhibitor of the mammalian replication initiation complex, inhibits replication from oriP. Therefore, ORC and the human replication initiation apparatus is required for replication from a viral origin of replication. SN - 0092-8674 UR - https://www.unboundmedicine.com/medline/citation/11509178/Replication_from_oriP_of_Epstein_Barr_virus_requires_human_ORC_and_is_inhibited_by_geminin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0092-8674(01)00458-5 DB - PRIME DP - Unbound Medicine ER -