Tags

Type your tag names separated by a space and hit enter

Muc-1, integrin, and osteopontin expression during the implantation cascade in sheep.
Biol Reprod. 2001 Sep; 65(3):820-8.BR

Abstract

The extracellular matrix protein osteopontin (OPN) is a component of histotroph that increases in uterine flushings from pregnant ewes during the peri-implantation period and is localized on the apical surfaces of the uterine luminal epithelium (LE) and conceptus trophectoderm (Tr). The potential involvement of OPN in the implantation adhesion cascade in sheep was investigated by examining temporal, spatial, and potential functional relationships between OPN, Muc-1, and integrin subunits during the estrous cycle and early pregnancy. Immunoreactive Muc-1 was highly expressed at the apical surfaces of uterine luminal (LE) and glandular epithelium (GE) in both cycling and pregnant ewes but was decreased dramatically on LE by Day 9 and was nearly undetectable by Day 17 of pregnancy when intimate contact between LE and Tr begins. In contrast, integrin subunits alpha(v), alpha(4), alpha(5), beta(1), beta(3), and beta(5) were constitutively expressed on conceptus Tr and at the apical surface of uterine LE and GE in both cyclic and early pregnant ewes. The apical expression of these subunits could contribute to the apical assembly of several OPN receptors including the alpha(v)beta(3), alpha(v)beta(1), alpha(v)beta(5), alpha(4)beta(1), and alpha(5)beta(1) heterodimers on endometrial LE and GE, and conceptus Tr in sheep. Functional analysis of potential OPN interactions with conceptus and endometrial integrins was performed on LE and Tr cells in vitro using beads coated with OPN, poly-L-lysine, or recombinant OPN in which the Arg-Gly-Asp sequence was replaced with RGE or RAD. Transmembrane accumulation of talin or alpha-actinin at the apical surface of uterine LE and conceptus Tr cells in contact with OPN-coated beads revealed functional integrin activation and cytoskeletal reorganization in response to OPN binding. These results provide a physiological framework for the role of OPN, a potential mediator of implantation in sheep, as a bridge between integrin heterodimers expressed by Tr and uterine LE responsible for adhesion for initial conceptus attachment.

Authors+Show Affiliations

Center for Animal Biotechnology and Genomics, Texas A&M University, College Station, TX 77843-2471, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11514347

Citation

Johnson, G A., et al. "Muc-1, Integrin, and Osteopontin Expression During the Implantation Cascade in Sheep." Biology of Reproduction, vol. 65, no. 3, 2001, pp. 820-8.
Johnson GA, Bazer FW, Jaeger LA, et al. Muc-1, integrin, and osteopontin expression during the implantation cascade in sheep. Biol Reprod. 2001;65(3):820-8.
Johnson, G. A., Bazer, F. W., Jaeger, L. A., Ka, H., Garlow, J. E., Pfarrer, C., Spencer, T. E., & Burghardt, R. C. (2001). Muc-1, integrin, and osteopontin expression during the implantation cascade in sheep. Biology of Reproduction, 65(3), 820-8.
Johnson GA, et al. Muc-1, Integrin, and Osteopontin Expression During the Implantation Cascade in Sheep. Biol Reprod. 2001;65(3):820-8. PubMed PMID: 11514347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Muc-1, integrin, and osteopontin expression during the implantation cascade in sheep. AU - Johnson,G A, AU - Bazer,F W, AU - Jaeger,L A, AU - Ka,H, AU - Garlow,J E, AU - Pfarrer,C, AU - Spencer,T E, AU - Burghardt,R C, PY - 2001/8/22/pubmed PY - 2002/1/5/medline PY - 2001/8/22/entrez SP - 820 EP - 8 JF - Biology of reproduction JO - Biol Reprod VL - 65 IS - 3 N2 - The extracellular matrix protein osteopontin (OPN) is a component of histotroph that increases in uterine flushings from pregnant ewes during the peri-implantation period and is localized on the apical surfaces of the uterine luminal epithelium (LE) and conceptus trophectoderm (Tr). The potential involvement of OPN in the implantation adhesion cascade in sheep was investigated by examining temporal, spatial, and potential functional relationships between OPN, Muc-1, and integrin subunits during the estrous cycle and early pregnancy. Immunoreactive Muc-1 was highly expressed at the apical surfaces of uterine luminal (LE) and glandular epithelium (GE) in both cycling and pregnant ewes but was decreased dramatically on LE by Day 9 and was nearly undetectable by Day 17 of pregnancy when intimate contact between LE and Tr begins. In contrast, integrin subunits alpha(v), alpha(4), alpha(5), beta(1), beta(3), and beta(5) were constitutively expressed on conceptus Tr and at the apical surface of uterine LE and GE in both cyclic and early pregnant ewes. The apical expression of these subunits could contribute to the apical assembly of several OPN receptors including the alpha(v)beta(3), alpha(v)beta(1), alpha(v)beta(5), alpha(4)beta(1), and alpha(5)beta(1) heterodimers on endometrial LE and GE, and conceptus Tr in sheep. Functional analysis of potential OPN interactions with conceptus and endometrial integrins was performed on LE and Tr cells in vitro using beads coated with OPN, poly-L-lysine, or recombinant OPN in which the Arg-Gly-Asp sequence was replaced with RGE or RAD. Transmembrane accumulation of talin or alpha-actinin at the apical surface of uterine LE and conceptus Tr cells in contact with OPN-coated beads revealed functional integrin activation and cytoskeletal reorganization in response to OPN binding. These results provide a physiological framework for the role of OPN, a potential mediator of implantation in sheep, as a bridge between integrin heterodimers expressed by Tr and uterine LE responsible for adhesion for initial conceptus attachment. SN - 0006-3363 UR - https://www.unboundmedicine.com/medline/citation/11514347/Muc_1_integrin_and_osteopontin_expression_during_the_implantation_cascade_in_sheep_ L2 - https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod65.3.820 DB - PRIME DP - Unbound Medicine ER -