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Human DNA replication initiation factors, ORC and MCM, associate with oriP of Epstein-Barr virus.
Proc Natl Acad Sci U S A. 2001 Aug 28; 98(18):10085-9.PN

Abstract

The 165-kb chromosome of Epstein-Barr virus (EBV) is replicated by cellular enzymes only once per cell cycle in human cells that are latently infected. Here, we report that the human origin recognition complex, ORC, can be detected in association with an EBV replication origin, oriP, in cells by using antibodies against three different subunits of human ORC to precipitate crosslinked chromatin. Mcm2, a subunit of the MCM replication licensing complex, was found to associate with oriP during G(1) and to dissociate from it during S phase. The detection of ORC and Mcm2 at oriP was shown to require the presence of the 120-bp replicator of oriP. Licensing and initiation of replication at oriP of EBV thus seem to be mediated by ORC. This is an example of a virus apparently using ORC and associated factors for the propagation of its genome.

Authors+Show Affiliations

Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11517328

Citation

Chaudhuri, B, et al. "Human DNA Replication Initiation Factors, ORC and MCM, Associate With oriP of Epstein-Barr Virus." Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 18, 2001, pp. 10085-9.
Chaudhuri B, Xu H, Todorov I, et al. Human DNA replication initiation factors, ORC and MCM, associate with oriP of Epstein-Barr virus. Proc Natl Acad Sci U S A. 2001;98(18):10085-9.
Chaudhuri, B., Xu, H., Todorov, I., Dutta, A., & Yates, J. L. (2001). Human DNA replication initiation factors, ORC and MCM, associate with oriP of Epstein-Barr virus. Proceedings of the National Academy of Sciences of the United States of America, 98(18), 10085-9.
Chaudhuri B, et al. Human DNA Replication Initiation Factors, ORC and MCM, Associate With oriP of Epstein-Barr Virus. Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10085-9. PubMed PMID: 11517328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human DNA replication initiation factors, ORC and MCM, associate with oriP of Epstein-Barr virus. AU - Chaudhuri,B, AU - Xu,H, AU - Todorov,I, AU - Dutta,A, AU - Yates,J L, Y1 - 2001/08/21/ PY - 2001/8/23/pubmed PY - 2001/10/5/medline PY - 2001/8/23/entrez SP - 10085 EP - 9 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 98 IS - 18 N2 - The 165-kb chromosome of Epstein-Barr virus (EBV) is replicated by cellular enzymes only once per cell cycle in human cells that are latently infected. Here, we report that the human origin recognition complex, ORC, can be detected in association with an EBV replication origin, oriP, in cells by using antibodies against three different subunits of human ORC to precipitate crosslinked chromatin. Mcm2, a subunit of the MCM replication licensing complex, was found to associate with oriP during G(1) and to dissociate from it during S phase. The detection of ORC and Mcm2 at oriP was shown to require the presence of the 120-bp replicator of oriP. Licensing and initiation of replication at oriP of EBV thus seem to be mediated by ORC. This is an example of a virus apparently using ORC and associated factors for the propagation of its genome. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/11517328/Human_DNA_replication_initiation_factors_ORC_and_MCM_associate_with_oriP_of_Epstein_Barr_virus_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=11517328 DB - PRIME DP - Unbound Medicine ER -