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Biosynthesis and structural characterization of medium-chain-length poly(3-hydroxyalkanoates) produced by Pseudomonas aeruginosa from fatty acids.
Int J Biol Macromol. 2001 Aug 20; 29(2):107-14.IJ

Abstract

In this study, we investigated the ability of Pseudomonas aeruginosa ATCC 27853 to grow and synthesize poly(3-hydroxyalkanoates) (PHAs) from saturated fatty acids with an even number of carbon atoms, from eight to 22, and from oleic acid. In a non-limiting medium, all carbon sources but docosanoic acid supported cell growth and PHA production, with eicosanoic acid giving the highest yield. In magnesium-limiting conditions, higher yields were obtained from sources with up to 16 carbon atoms. Composition was estimated by gas chromatography of methanolyzed samples and (13)C nuclear magnetic resonance. The 3-hydroxyalkanoate units extended from hexanoate to tetradecanoate or tetradecenoate, with octanoate and decanoate as the predominant components. Weight average molecular weights ranged from 78,000 to 316,000. Fast atom bombardment mass spectrometry of partially pyrolyzed samples, coupled to statistical analysis, showed that these PHAs are random copolymers.

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Authors+Show Affiliations

Ballistreri A
Dipartimento di Scienze Chimiche, Università di Catania, 95125 Catania, Italy.
Giuffrida M
No affiliation info available
Guglielmino SP
No affiliation info available
Carnazza S
No affiliation info available
Ferreri A
No affiliation info available
Impallomeni G
No affiliation info available

MeSH

Chromatography, GasFatty AcidsMacromolecular SubstancesMagnetic Resonance SpectroscopyMolecular StructureMolecular WeightPolyestersPseudomonas aeruginosaSpectrometry, Mass, Fast Atom Bombardment

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11518582

Citation

Ballistreri, A, et al. "Biosynthesis and Structural Characterization of Medium-chain-length Poly(3-hydroxyalkanoates) Produced By Pseudomonas Aeruginosa From Fatty Acids." International Journal of Biological Macromolecules, vol. 29, no. 2, 2001, pp. 107-14.
Ballistreri A, Giuffrida M, Guglielmino SP, et al. Biosynthesis and structural characterization of medium-chain-length poly(3-hydroxyalkanoates) produced by Pseudomonas aeruginosa from fatty acids. Int J Biol Macromol. 2001;29(2):107-14.
Ballistreri, A., Giuffrida, M., Guglielmino, S. P., Carnazza, S., Ferreri, A., & Impallomeni, G. (2001). Biosynthesis and structural characterization of medium-chain-length poly(3-hydroxyalkanoates) produced by Pseudomonas aeruginosa from fatty acids. International Journal of Biological Macromolecules, 29(2), 107-14.
Ballistreri A, et al. Biosynthesis and Structural Characterization of Medium-chain-length Poly(3-hydroxyalkanoates) Produced By Pseudomonas Aeruginosa From Fatty Acids. Int J Biol Macromol. 2001 Aug 20;29(2):107-14. PubMed PMID: 11518582.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biosynthesis and structural characterization of medium-chain-length poly(3-hydroxyalkanoates) produced by Pseudomonas aeruginosa from fatty acids. AU - Ballistreri,A, AU - Giuffrida,M, AU - Guglielmino,S P, AU - Carnazza,S, AU - Ferreri,A, AU - Impallomeni,G, PY - 2001/8/24/pubmed PY - 2002/1/5/medline PY - 2001/8/24/entrez SP - 107 EP - 14 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 29 IS - 2 N2 - In this study, we investigated the ability of Pseudomonas aeruginosa ATCC 27853 to grow and synthesize poly(3-hydroxyalkanoates) (PHAs) from saturated fatty acids with an even number of carbon atoms, from eight to 22, and from oleic acid. In a non-limiting medium, all carbon sources but docosanoic acid supported cell growth and PHA production, with eicosanoic acid giving the highest yield. In magnesium-limiting conditions, higher yields were obtained from sources with up to 16 carbon atoms. Composition was estimated by gas chromatography of methanolyzed samples and (13)C nuclear magnetic resonance. The 3-hydroxyalkanoate units extended from hexanoate to tetradecanoate or tetradecenoate, with octanoate and decanoate as the predominant components. Weight average molecular weights ranged from 78,000 to 316,000. Fast atom bombardment mass spectrometry of partially pyrolyzed samples, coupled to statistical analysis, showed that these PHAs are random copolymers. SN - 0141-8130 UR - https://www.unboundmedicine.com/medline/citation/11518582/Biosynthesis_and_structural_characterization_of_medium_chain_length_poly_3_hydroxyalkanoates__produced_by_Pseudomonas_aeruginosa_from_fatty_acids_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(01)00154-4 DB - PRIME DP - Unbound Medicine ER -