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Effects of branched-chain amino acids on placental amino acid transfer and insulin and glucagon release in the ovine fetus.

Abstract

OBJECTIVE

Competition for placental amino acid transporters can affect the fetal supply of amino acids. Specifically, the branched-chain amino acids-isoleucine, leucine, and valine-may inhibit the transfer of other amino acids. This study was undertaken to determine the effect of branched-chain amino acids on the umbilical uptake of amino acids.

STUDY DESIGN

Six late-gestation ewes were infused sequentially for 2 hours with 3 different mixtures of amino acids: (1) one that was comparable to commercial parenteral nutrition preparations, (2) the same solution without branched-chain amino acids, and (3) branched-chain amino acids alone. Maternal and fetal blood samples were collected simultaneously for the determination of uterine and umbilical uptake values of amino acids, and for concentrations of arterial insulin, glucagon, glucose, and lactate before (control) and during (experimental) infusion.

RESULTS

Umbilical uptake of branched-chain amino acids increased significantly when they were present in the infusates. The fetal uptake of several other amino acids could be increased by increasing their maternal concentrations. Inhibition of umbilical uptake by branched-chain amino acids could be shown for threonine and methionine. The infusion of branched-chain amino acids alone did not affect maternal and fetal insulin or glucagon concentrations.

CONCLUSIONS

In late-gestation sheep, an increase in maternal plasma concentration of branched-chain amino acids led to increased branched-chain amino acid umbilical uptake, but branched-chain amino acids can also inhibit the transport of some amino acids to the fetus. Changes in fetal plasma concentration and uptake of branched-chain amino acid appear to have no significant effect on fetal insulin or glucagon.

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  • Authors+Show Affiliations

    ,

    Department of Gynecology, Bialystok Medical University, Poland.

    , , , , ,

    Source

    MeSH

    Amino Acids
    Amino Acids, Branched-Chain
    Animals
    Arteries
    Biological Transport
    Blood Glucose
    Female
    Fetal Blood
    Glucagon
    Insulin
    Isoleucine
    Kinetics
    Lactic Acid
    Leucine
    Maternal-Fetal Exchange
    Oxygen Consumption
    Placenta
    Pregnancy
    Sheep
    Umbilical Veins
    Uterus
    Valine
    Veins

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't
    Research Support, U.S. Gov't, P.H.S.

    Language

    eng

    PubMed ID

    11518915

    Citation

    Józwik, M, et al. "Effects of Branched-chain Amino Acids On Placental Amino Acid Transfer and Insulin and Glucagon Release in the Ovine Fetus." American Journal of Obstetrics and Gynecology, vol. 185, no. 2, 2001, pp. 487-95.
    Józwik M, Teng C, Wilkening RB, et al. Effects of branched-chain amino acids on placental amino acid transfer and insulin and glucagon release in the ovine fetus. Am J Obstet Gynecol. 2001;185(2):487-95.
    Józwik, M., Teng, C., Wilkening, R. B., Meschia, G., Tooze, J., Chung, M., & Battaglia, F. C. (2001). Effects of branched-chain amino acids on placental amino acid transfer and insulin and glucagon release in the ovine fetus. American Journal of Obstetrics and Gynecology, 185(2), pp. 487-95.
    Józwik M, et al. Effects of Branched-chain Amino Acids On Placental Amino Acid Transfer and Insulin and Glucagon Release in the Ovine Fetus. Am J Obstet Gynecol. 2001;185(2):487-95. PubMed PMID: 11518915.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effects of branched-chain amino acids on placental amino acid transfer and insulin and glucagon release in the ovine fetus. AU - Józwik,M, AU - Teng,C, AU - Wilkening,R B, AU - Meschia,G, AU - Tooze,J, AU - Chung,M, AU - Battaglia,F C, PY - 2001/8/24/pubmed PY - 2001/9/14/medline PY - 2001/8/24/entrez SP - 487 EP - 95 JF - American journal of obstetrics and gynecology JO - Am. J. Obstet. Gynecol. VL - 185 IS - 2 N2 - OBJECTIVE: Competition for placental amino acid transporters can affect the fetal supply of amino acids. Specifically, the branched-chain amino acids-isoleucine, leucine, and valine-may inhibit the transfer of other amino acids. This study was undertaken to determine the effect of branched-chain amino acids on the umbilical uptake of amino acids. STUDY DESIGN: Six late-gestation ewes were infused sequentially for 2 hours with 3 different mixtures of amino acids: (1) one that was comparable to commercial parenteral nutrition preparations, (2) the same solution without branched-chain amino acids, and (3) branched-chain amino acids alone. Maternal and fetal blood samples were collected simultaneously for the determination of uterine and umbilical uptake values of amino acids, and for concentrations of arterial insulin, glucagon, glucose, and lactate before (control) and during (experimental) infusion. RESULTS: Umbilical uptake of branched-chain amino acids increased significantly when they were present in the infusates. The fetal uptake of several other amino acids could be increased by increasing their maternal concentrations. Inhibition of umbilical uptake by branched-chain amino acids could be shown for threonine and methionine. The infusion of branched-chain amino acids alone did not affect maternal and fetal insulin or glucagon concentrations. CONCLUSIONS: In late-gestation sheep, an increase in maternal plasma concentration of branched-chain amino acids led to increased branched-chain amino acid umbilical uptake, but branched-chain amino acids can also inhibit the transport of some amino acids to the fetus. Changes in fetal plasma concentration and uptake of branched-chain amino acid appear to have no significant effect on fetal insulin or glucagon. SN - 0002-9378 UR - https://www.unboundmedicine.com/medline/citation/11518915/Effects_of_branched_chain_amino_acids_on_placental_amino_acid_transfer_and_insulin_and_glucagon_release_in_the_ovine_fetus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0002-9378(01)22354-0 DB - PRIME DP - Unbound Medicine ER -