Tags

Type your tag names separated by a space and hit enter

Detection of specific antibodies using immunosubtraction and capillary electrophoresis instrumentation.
Electrophoresis. 2001 Aug; 22(12):2391-7.E

Abstract

Solution-phase immunoassays based on capillary electrophoresis (CE) separations have been shown to be rapid and simple to perform. The potential for sample matrix interference and incompatibility with multiplexing conditions for antibody detection when dealing with real samples, however, has prompted the development of an assay that utilizes an immunosubtraction methodology. A model assay for the detection of specific antibodies that relies on solid-phase extraction, CE and laser-induced fluorescence (LIF) detection is described. The method, called immunocapture-immunosubtraction (ICIS), incorporates an antibody capture/purification protocol using magnetic particles. The detection of specific antibodies is achieved by CE-LIF analysis of a probe solution following incubation with the captured antibodies. As an example of the ICIS assay's capabilities, the relative quantification of anti-fluorescein in serum is presented.

Authors+Show Affiliations

Paquette DM
Department of Chemistry and Biochemistry, Concordia University, Montreal, Quebec, Canada. dm_paqu@alcor.concordia.ca
Banks PR
No affiliation info available

MeSH

AnimalsAntibodiesAntibody SpecificityAntigen-Antibody ComplexCalibrationElectrophoresis, CapillaryFeasibility StudiesFluoresceinFluorescent DyesFluorometryImmunoassayImmunomagnetic SeparationImmunosorbent TechniquesLasersRabbitsSubtraction Technique

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11519941

Citation

Paquette, D M., and P R. Banks. "Detection of Specific Antibodies Using Immunosubtraction and Capillary Electrophoresis Instrumentation." Electrophoresis, vol. 22, no. 12, 2001, pp. 2391-7.
Paquette DM, Banks PR. Detection of specific antibodies using immunosubtraction and capillary electrophoresis instrumentation. Electrophoresis. 2001;22(12):2391-7.
Paquette, D. M., & Banks, P. R. (2001). Detection of specific antibodies using immunosubtraction and capillary electrophoresis instrumentation. Electrophoresis, 22(12), 2391-7.
Paquette DM, Banks PR. Detection of Specific Antibodies Using Immunosubtraction and Capillary Electrophoresis Instrumentation. Electrophoresis. 2001;22(12):2391-7. PubMed PMID: 11519941.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of specific antibodies using immunosubtraction and capillary electrophoresis instrumentation. AU - Paquette,D M, AU - Banks,P R, PY - 2001/8/25/pubmed PY - 2002/2/8/medline PY - 2001/8/25/entrez SP - 2391 EP - 7 JF - Electrophoresis JO - Electrophoresis VL - 22 IS - 12 N2 - Solution-phase immunoassays based on capillary electrophoresis (CE) separations have been shown to be rapid and simple to perform. The potential for sample matrix interference and incompatibility with multiplexing conditions for antibody detection when dealing with real samples, however, has prompted the development of an assay that utilizes an immunosubtraction methodology. A model assay for the detection of specific antibodies that relies on solid-phase extraction, CE and laser-induced fluorescence (LIF) detection is described. The method, called immunocapture-immunosubtraction (ICIS), incorporates an antibody capture/purification protocol using magnetic particles. The detection of specific antibodies is achieved by CE-LIF analysis of a probe solution following incubation with the captured antibodies. As an example of the ICIS assay's capabilities, the relative quantification of anti-fluorescein in serum is presented. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/11519941/Detection_of_specific_antibodies_using_immunosubtraction_and_capillary_electrophoresis_instrumentation_ L2 - https://doi.org/10.1002/1522-2683(200107)22:12<2391::AID-ELPS2391>3.0.CO;2-B DB - PRIME DP - Unbound Medicine ER -