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Capillaries modified by noncovalent anionic polymer adsorption for capillary zone electrophoresis, micellar electrokinetic capillary chromatography and capillary electrophoresis mass spectrometry.
Electrophoresis. 2001 Aug; 22(12):2565-73.E

Abstract

A simple coating procedure for generation of a high and pH-independent electroosmotic flow in capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MEKC) is described. The bilayer coating was formed by noncovalent adsorption of the ionic polymers Polybrene and poly(vinylsulfonate) (PVS). A stable dynamic coating was formed when PVS was added to the background electrolyte. Thus, when the PVS concentration in the background electrolyte was optimized for CZE (0.01%), the EOF differed less than 0.3% after 54 runs. The electroosmotic mobility in the coated capillaries was (4.9+/-0.1) x 10(-4) cm2V(-1)s(-1) in a pH-range of 2-10 (ionic strength = 30 mM). When alkaline compounds were used as test substances intracapillary and intercapillary migration time variations (n = 6) were less than 1% relative standard deviation (RSD) and 2% RSD, respectively in the entire pH range. The coating was fairly stable in the presence of sodium dodecyl sulfate, and this made it possible to perform fast MEKC separations at low pH. When neutral compounds were used as test substances, the intracapillary migration time variations (n = 6) were less than 2% RSD in a pH range of 2-9. In addition to fast CZE and MEKC separations at low pH, analysis of the alkaline compounds by CE-MS was also possible.

Authors+Show Affiliations

Bendahl L
Department of Analytical and Pharmaceutical Chemistry, Royal Danish School of Pharmacy, Copenhagen, Denmark. labe@mail.dfh.dk
Hansen SH
No affiliation info available
Gammelgaard B
No affiliation info available

MeSH

AdsorptionAnionsChromatography, Micellar Electrokinetic CapillaryElectrolytesElectrophoresis, CapillaryHexadimethrine BromideHydrogen-Ion ConcentrationMass SpectrometryPolymersPolyvinylsReproducibility of ResultsSodium Dodecyl SulfateSulfonic AcidsTime Factors

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

11519960

Citation

Bendahl, L, et al. "Capillaries Modified By Noncovalent Anionic Polymer Adsorption for Capillary Zone Electrophoresis, Micellar Electrokinetic Capillary Chromatography and Capillary Electrophoresis Mass Spectrometry." Electrophoresis, vol. 22, no. 12, 2001, pp. 2565-73.
Bendahl L, Hansen SH, Gammelgaard B. Capillaries modified by noncovalent anionic polymer adsorption for capillary zone electrophoresis, micellar electrokinetic capillary chromatography and capillary electrophoresis mass spectrometry. Electrophoresis. 2001;22(12):2565-73.
Bendahl, L., Hansen, S. H., & Gammelgaard, B. (2001). Capillaries modified by noncovalent anionic polymer adsorption for capillary zone electrophoresis, micellar electrokinetic capillary chromatography and capillary electrophoresis mass spectrometry. Electrophoresis, 22(12), 2565-73.
Bendahl L, Hansen SH, Gammelgaard B. Capillaries Modified By Noncovalent Anionic Polymer Adsorption for Capillary Zone Electrophoresis, Micellar Electrokinetic Capillary Chromatography and Capillary Electrophoresis Mass Spectrometry. Electrophoresis. 2001;22(12):2565-73. PubMed PMID: 11519960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillaries modified by noncovalent anionic polymer adsorption for capillary zone electrophoresis, micellar electrokinetic capillary chromatography and capillary electrophoresis mass spectrometry. AU - Bendahl,L, AU - Hansen,S H, AU - Gammelgaard,B, PY - 2001/8/25/pubmed PY - 2002/2/8/medline PY - 2001/8/25/entrez SP - 2565 EP - 73 JF - Electrophoresis JO - Electrophoresis VL - 22 IS - 12 N2 - A simple coating procedure for generation of a high and pH-independent electroosmotic flow in capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MEKC) is described. The bilayer coating was formed by noncovalent adsorption of the ionic polymers Polybrene and poly(vinylsulfonate) (PVS). A stable dynamic coating was formed when PVS was added to the background electrolyte. Thus, when the PVS concentration in the background electrolyte was optimized for CZE (0.01%), the EOF differed less than 0.3% after 54 runs. The electroosmotic mobility in the coated capillaries was (4.9+/-0.1) x 10(-4) cm2V(-1)s(-1) in a pH-range of 2-10 (ionic strength = 30 mM). When alkaline compounds were used as test substances intracapillary and intercapillary migration time variations (n = 6) were less than 1% relative standard deviation (RSD) and 2% RSD, respectively in the entire pH range. The coating was fairly stable in the presence of sodium dodecyl sulfate, and this made it possible to perform fast MEKC separations at low pH. When neutral compounds were used as test substances, the intracapillary migration time variations (n = 6) were less than 2% RSD in a pH range of 2-9. In addition to fast CZE and MEKC separations at low pH, analysis of the alkaline compounds by CE-MS was also possible. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/11519960/Capillaries_modified_by_noncovalent_anionic_polymer_adsorption_for_capillary_zone_electrophoresis_micellar_electrokinetic_capillary_chromatography_and_capillary_electrophoresis_mass_spectrometry_ L2 - https://doi.org/10.1002/1522-2683(200107)22:12<2565::AID-ELPS2565>3.0.CO;2-I DB - PRIME DP - Unbound Medicine ER -