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Synergistic induction of t-PA by vascular endothelial growth factor and basic fibroblast growth factor and localization of t-PA to Weibel-Palade bodies in bovine microvascular endothelial cells.
Thromb Haemost. 2001 Aug; 86(2):702-9.TH

Abstract

Endothelial cell migration is stimulated by members of the vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) families, and is dependent on extracellular proteolytic activity provided by enzymes of the plasminogen activator (PA) system. Here we report that in bovine microvascular endothelial cells (BME cells), bFGF principally increased urokinase-type PA (u-PA) while tissue-type PA (t-PA) was increased mainly by VEGF. In bovine aortic endothelial cells (BAE cells), bFGF increased u-PA, whereas VEGF had no effect. Co-added bFGF and VEGF increased t-PA mRNA levels and enzyme activity in both cell types in a synergistic manner. Tissue-type plasminogen activator (t-PA) immunoreactivity colocalized with von Willebrand factor, a marker for Weibel-Palade bodies. Co-added bFGF and VEGF increased the number of t-PA-positive cells as well as the number of t-PA-positive granules per cell. Localization of t-PA in regulated storage granules endows endothelial cells with the potential to rapidly increase proteolytic activity in the pericellular environment.

Authors+Show Affiliations

Department of Morphology, University Medical Center, Geneva, Switzerland. michael.pepper@medecine.unige.chNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11522025

Citation

Pepper, M S., et al. "Synergistic Induction of t-PA By Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor and Localization of t-PA to Weibel-Palade Bodies in Bovine Microvascular Endothelial Cells." Thrombosis and Haemostasis, vol. 86, no. 2, 2001, pp. 702-9.
Pepper MS, Rosnoblet C, Di Sanza C, et al. Synergistic induction of t-PA by vascular endothelial growth factor and basic fibroblast growth factor and localization of t-PA to Weibel-Palade bodies in bovine microvascular endothelial cells. Thromb Haemost. 2001;86(2):702-9.
Pepper, M. S., Rosnoblet, C., Di Sanza, C., & Kruithof, E. K. (2001). Synergistic induction of t-PA by vascular endothelial growth factor and basic fibroblast growth factor and localization of t-PA to Weibel-Palade bodies in bovine microvascular endothelial cells. Thrombosis and Haemostasis, 86(2), 702-9.
Pepper MS, et al. Synergistic Induction of t-PA By Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor and Localization of t-PA to Weibel-Palade Bodies in Bovine Microvascular Endothelial Cells. Thromb Haemost. 2001;86(2):702-9. PubMed PMID: 11522025.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synergistic induction of t-PA by vascular endothelial growth factor and basic fibroblast growth factor and localization of t-PA to Weibel-Palade bodies in bovine microvascular endothelial cells. AU - Pepper,M S, AU - Rosnoblet,C, AU - Di Sanza,C, AU - Kruithof,E K, PY - 2001/8/28/pubmed PY - 2002/8/3/medline PY - 2001/8/28/entrez SP - 702 EP - 9 JF - Thrombosis and haemostasis JO - Thromb Haemost VL - 86 IS - 2 N2 - Endothelial cell migration is stimulated by members of the vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) families, and is dependent on extracellular proteolytic activity provided by enzymes of the plasminogen activator (PA) system. Here we report that in bovine microvascular endothelial cells (BME cells), bFGF principally increased urokinase-type PA (u-PA) while tissue-type PA (t-PA) was increased mainly by VEGF. In bovine aortic endothelial cells (BAE cells), bFGF increased u-PA, whereas VEGF had no effect. Co-added bFGF and VEGF increased t-PA mRNA levels and enzyme activity in both cell types in a synergistic manner. Tissue-type plasminogen activator (t-PA) immunoreactivity colocalized with von Willebrand factor, a marker for Weibel-Palade bodies. Co-added bFGF and VEGF increased the number of t-PA-positive cells as well as the number of t-PA-positive granules per cell. Localization of t-PA in regulated storage granules endows endothelial cells with the potential to rapidly increase proteolytic activity in the pericellular environment. SN - 0340-6245 UR - https://www.unboundmedicine.com/medline/citation/11522025/Synergistic_induction_of_t_PA_by_vascular_endothelial_growth_factor_and_basic_fibroblast_growth_factor_and_localization_of_t_PA_to_Weibel_Palade_bodies_in_bovine_microvascular_endothelial_cells_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=11522025.ui DB - PRIME DP - Unbound Medicine ER -