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Differentiation of monoclonal gammopathy of undetermined significance and multiple myeloma using flow cytometric characteristics of plasma cells.
Haematologica. 2001 Aug; 86(8):837-43.H

Abstract

BACKGROUND AND OBJECTIVES

The differential diagnosis between multiple myeloma (MM) and monoclonal gammopathy of undetermined significance (MGUS) may be uncertain in some cases; this problem is reflected by discrepancies between different classification systems with an accordance in only 2/3 of cases. We studied whether flow-cytometric characteristics of plasma cells (PC) can be used for the differentiation between MGUS and MM.

DESIGN AND METHODS

Patients were divided into 3 groups: Group A included 13 myeloma patients with a plasma cell infiltration of the bone marrow of 10-30%, serum M-protein < or = 3.5 g/dL (IgG) or < or = 2 g/dl (IgA) and without bone lesions in conventional radiography. Group B consisted of 53 patients who fulfilled the Durie and Salmon diagnostic criteria including at least one major criterion, and group C individuals with MGUS (n=17). The ratio of immunophenotypically normal (i.e. CD19(+)/CD56(-)) to all bone marrow plasma cells (BMPC), the number of peripheral blood PC (PBPC), the percentage of BMPC in S-phase and the DNA content of BMPC were analyzed.

RESULTS

All individuals with MGUS and no patient with MM in group A or group B had a ratio of phenotypically normal to all BMPC > or = 20%. The median of monoclonal PBPC was 0/microL (range 0-2/ microL) in MGUS, 1/microL (range 0-30/microL) in MM group A and 2.4/microL (range 0-211/microL) in MM group B. The median percentage of BMPC in S-phase was 1.6% both in MGUS and in group A and 3% in group B. Aneuploidy was found in 12%, 11% and 41% in MGUS, group A and group B, respectively.

INTERPRETATION AND CONCLUSIONS

The ratio of immunophenotypically normal to all BMPC was the only flow-cytometric parameter for the differentiation of MGUS and MM group A (p<0.0005). The other parameters were significantly different between MGUS and MM group B, but not group A.

Authors+Show Affiliations

Department of Hematology and Oncology, Universitätsklinikum Charité, Humboldt-Universität Berlin, Germany. sezer@charite.deNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11522540

Citation

Sezer, O, et al. "Differentiation of Monoclonal Gammopathy of Undetermined Significance and Multiple Myeloma Using Flow Cytometric Characteristics of Plasma Cells." Haematologica, vol. 86, no. 8, 2001, pp. 837-43.
Sezer O, Heider U, Zavrski I, et al. Differentiation of monoclonal gammopathy of undetermined significance and multiple myeloma using flow cytometric characteristics of plasma cells. Haematologica. 2001;86(8):837-43.
Sezer, O., Heider, U., Zavrski, I., & Possinger, K. (2001). Differentiation of monoclonal gammopathy of undetermined significance and multiple myeloma using flow cytometric characteristics of plasma cells. Haematologica, 86(8), 837-43.
Sezer O, et al. Differentiation of Monoclonal Gammopathy of Undetermined Significance and Multiple Myeloma Using Flow Cytometric Characteristics of Plasma Cells. Haematologica. 2001;86(8):837-43. PubMed PMID: 11522540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differentiation of monoclonal gammopathy of undetermined significance and multiple myeloma using flow cytometric characteristics of plasma cells. AU - Sezer,O, AU - Heider,U, AU - Zavrski,I, AU - Possinger,K, PY - 2001/8/28/pubmed PY - 2002/9/26/medline PY - 2001/8/28/entrez SP - 837 EP - 43 JF - Haematologica JO - Haematologica VL - 86 IS - 8 N2 - BACKGROUND AND OBJECTIVES: The differential diagnosis between multiple myeloma (MM) and monoclonal gammopathy of undetermined significance (MGUS) may be uncertain in some cases; this problem is reflected by discrepancies between different classification systems with an accordance in only 2/3 of cases. We studied whether flow-cytometric characteristics of plasma cells (PC) can be used for the differentiation between MGUS and MM. DESIGN AND METHODS: Patients were divided into 3 groups: Group A included 13 myeloma patients with a plasma cell infiltration of the bone marrow of 10-30%, serum M-protein < or = 3.5 g/dL (IgG) or < or = 2 g/dl (IgA) and without bone lesions in conventional radiography. Group B consisted of 53 patients who fulfilled the Durie and Salmon diagnostic criteria including at least one major criterion, and group C individuals with MGUS (n=17). The ratio of immunophenotypically normal (i.e. CD19(+)/CD56(-)) to all bone marrow plasma cells (BMPC), the number of peripheral blood PC (PBPC), the percentage of BMPC in S-phase and the DNA content of BMPC were analyzed. RESULTS: All individuals with MGUS and no patient with MM in group A or group B had a ratio of phenotypically normal to all BMPC > or = 20%. The median of monoclonal PBPC was 0/microL (range 0-2/ microL) in MGUS, 1/microL (range 0-30/microL) in MM group A and 2.4/microL (range 0-211/microL) in MM group B. The median percentage of BMPC in S-phase was 1.6% both in MGUS and in group A and 3% in group B. Aneuploidy was found in 12%, 11% and 41% in MGUS, group A and group B, respectively. INTERPRETATION AND CONCLUSIONS: The ratio of immunophenotypically normal to all BMPC was the only flow-cytometric parameter for the differentiation of MGUS and MM group A (p<0.0005). The other parameters were significantly different between MGUS and MM group B, but not group A. SN - 0390-6078 UR - https://www.unboundmedicine.com/medline/citation/11522540/Differentiation_of_monoclonal_gammopathy_of_undetermined_significance_and_multiple_myeloma_using_flow_cytometric_characteristics_of_plasma_cells_ L2 - http://www.diseaseinfosearch.org/result/4962 DB - PRIME DP - Unbound Medicine ER -