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[The Thermus thermophilus 5S rRNA-protein complex: Identifications of specific binding sites for proteins L5 and L18 in 5S rRNA].
Mol Biol (Mosk). 2001 Jul-Aug; 35(4):610-6.MB

Abstract

Three 5S rRNA-binding ribosomal proteins (L5, L18, TL5) of extremely thermophilic bacterium Thermus thermophilus have earlier been isolated. Structural analysis of their complexes with rRNA requires identification of their binding sites in the 5S rRNA. Previously, a TL5-binding site has been identified, a TL5-RNA complex crystallized, and its structure determined to 2.3 A. The sites for L5 and L18 were characterized, and two corresponding 5S rRNA fragments constructed. Of these, a 34-nt fragment specifically interacted with L5, and a 55-nt fragment interacted with L5, L18, and with both proteins. The 34-nt fragment-L5 complex was crystallized; the crystals are suitable for high-resolution X-ray analysis.

Authors+Show Affiliations

Gongadze GM
gongadze@vega.protres.ru
Perederina AA
No affiliation info available
Meshcheriakov VA
No affiliation info available
Fedorov RV
No affiliation info available
Moskalenko SE
No affiliation info available
Rak AV
No affiliation info available
Serganov AA
No affiliation info available
Shcherbakov DV
No affiliation info available
Nikonov SV
No affiliation info available
Garber MB
No affiliation info available

MeSH

Binding SitesProtein BindingRNA, BacterialRNA, Ribosomal, 5SRibosomal ProteinsThermus thermophilus

Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

11524947

Citation

Gongadze, G M., et al. "[The Thermus Thermophilus 5S rRNA-protein Complex: Identifications of Specific Binding Sites for Proteins L5 and L18 in 5S RRNA]." Molekuliarnaia Biologiia, vol. 35, no. 4, 2001, pp. 610-6.
Gongadze GM, Perederina AA, Meshcheriakov VA, et al. [The Thermus thermophilus 5S rRNA-protein complex: Identifications of specific binding sites for proteins L5 and L18 in 5S rRNA]. Mol Biol (Mosk). 2001;35(4):610-6.
Gongadze, G. M., Perederina, A. A., Meshcheriakov, V. A., Fedorov, R. V., Moskalenko, S. E., Rak, A. V., Serganov, A. A., Shcherbakov, D. V., Nikonov, S. V., & Garber, M. B. (2001). [The Thermus thermophilus 5S rRNA-protein complex: Identifications of specific binding sites for proteins L5 and L18 in 5S rRNA]. Molekuliarnaia Biologiia, 35(4), 610-6.
Gongadze GM, et al. [The Thermus Thermophilus 5S rRNA-protein Complex: Identifications of Specific Binding Sites for Proteins L5 and L18 in 5S RRNA]. Mol Biol (Mosk). 2001 Jul-Aug;35(4):610-6. PubMed PMID: 11524947.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The Thermus thermophilus 5S rRNA-protein complex: Identifications of specific binding sites for proteins L5 and L18 in 5S rRNA]. AU - Gongadze,G M, AU - Perederina,A A, AU - Meshcheriakov,V A, AU - Fedorov,R V, AU - Moskalenko,S E, AU - Rak,A V, AU - Serganov,A A, AU - Shcherbakov,D V, AU - Nikonov,S V, AU - Garber,M B, PY - 2001/8/30/pubmed PY - 2002/1/17/medline PY - 2001/8/30/entrez SP - 610 EP - 6 JF - Molekuliarnaia biologiia JO - Mol Biol (Mosk) VL - 35 IS - 4 N2 - Three 5S rRNA-binding ribosomal proteins (L5, L18, TL5) of extremely thermophilic bacterium Thermus thermophilus have earlier been isolated. Structural analysis of their complexes with rRNA requires identification of their binding sites in the 5S rRNA. Previously, a TL5-binding site has been identified, a TL5-RNA complex crystallized, and its structure determined to 2.3 A. The sites for L5 and L18 were characterized, and two corresponding 5S rRNA fragments constructed. Of these, a 34-nt fragment specifically interacted with L5, and a 55-nt fragment interacted with L5, L18, and with both proteins. The 34-nt fragment-L5 complex was crystallized; the crystals are suitable for high-resolution X-ray analysis. SN - 0026-8984 UR - https://www.unboundmedicine.com/medline/citation/11524947/[The_Thermus_thermophilus_5S_rRNA_protein_complex:_Identifications_of_specific_binding_sites_for_proteins_L5_and_L18_in_5S_rRNA]_ L2 - https://antibodies.cancer.gov/detail/CPTC-NT5E+-1 DB - PRIME DP - Unbound Medicine ER -