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[Study of the binding of the S7 protein with 16S rRNA fragment 926-986/1219-1393 as a key step in the assembly of the small subunit of prokaryotic ribosomes].
Mol Biol (Mosk). 2001 Jul-Aug; 35(4):617-27.MB

Abstract

Both structural and thermodynamic studies are necessary to understand the ribosome assembly. An initial step was made in studying the interaction between a 16S rRNA fragment and S7, a key protein in assembling the prokaryotic ribosome small subunit. The apparent dissociation constant was obtained for complexes of recombinant Escherichia coli and Thermus thermophilus S7 with a fragment of the 3' domain of the E. coli 16S rRNA. Both proteins showed a high rRNA-binding activity, which was not observed earlier. Since RNA and proteins are conformationally labile, their folding must be considered to correctly describe the RNA-protein interactions.

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Authors+Show Affiliations

Rassokhin TI
Biology Departament, University of Virginia, Charlottesville, VA 22904-4775, USA.
Golovin AV
No affiliation info available
Petrova EB
No affiliation info available
Spiridonova VA
No affiliation info available
Karginova OA
No affiliation info available
Rozhdestvenskiĭ TS
No affiliation info available
Brosius J
No affiliation info available
Kopylov AM
No affiliation info available

MeSH

Amino Acid SequenceBacterial ProteinsBase SequenceEscherichia coliMolecular Sequence DataNucleic Acid ConformationProtein BindingProtein ConformationRNA, BacterialRNA, Ribosomal, 16SRecombinant ProteinsRibosomal ProteinsRibosomesThermus thermophilus

Pub Type(s)

Journal Article

Language

rus

PubMed ID

11524948

Citation

Rassokhin, T I., et al. "[Study of the Binding of the S7 Protein With 16S rRNA Fragment 926-986/1219-1393 as a Key Step in the Assembly of the Small Subunit of Prokaryotic Ribosomes]." Molekuliarnaia Biologiia, vol. 35, no. 4, 2001, pp. 617-27.
Rassokhin TI, Golovin AV, Petrova EB, et al. [Study of the binding of the S7 protein with 16S rRNA fragment 926-986/1219-1393 as a key step in the assembly of the small subunit of prokaryotic ribosomes]. Mol Biol (Mosk). 2001;35(4):617-27.
Rassokhin, T. I., Golovin, A. V., Petrova, E. B., Spiridonova, V. A., Karginova, O. A., Rozhdestvenskiĭ, T. S., Brosius, J., & Kopylov, A. M. (2001). [Study of the binding of the S7 protein with 16S rRNA fragment 926-986/1219-1393 as a key step in the assembly of the small subunit of prokaryotic ribosomes]. Molekuliarnaia Biologiia, 35(4), 617-27.
Rassokhin TI, et al. [Study of the Binding of the S7 Protein With 16S rRNA Fragment 926-986/1219-1393 as a Key Step in the Assembly of the Small Subunit of Prokaryotic Ribosomes]. Mol Biol (Mosk). 2001 Jul-Aug;35(4):617-27. PubMed PMID: 11524948.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Study of the binding of the S7 protein with 16S rRNA fragment 926-986/1219-1393 as a key step in the assembly of the small subunit of prokaryotic ribosomes]. AU - Rassokhin,T I, AU - Golovin,A V, AU - Petrova,E B, AU - Spiridonova,V A, AU - Karginova,O A, AU - Rozhdestvenskiĭ,T S, AU - Brosius,J, AU - Kopylov,A M, PY - 2001/8/30/pubmed PY - 2002/1/17/medline PY - 2001/8/30/entrez SP - 617 EP - 27 JF - Molekuliarnaia biologiia JO - Mol Biol (Mosk) VL - 35 IS - 4 N2 - Both structural and thermodynamic studies are necessary to understand the ribosome assembly. An initial step was made in studying the interaction between a 16S rRNA fragment and S7, a key protein in assembling the prokaryotic ribosome small subunit. The apparent dissociation constant was obtained for complexes of recombinant Escherichia coli and Thermus thermophilus S7 with a fragment of the 3' domain of the E. coli 16S rRNA. Both proteins showed a high rRNA-binding activity, which was not observed earlier. Since RNA and proteins are conformationally labile, their folding must be considered to correctly describe the RNA-protein interactions. SN - 0026-8984 UR - https://www.unboundmedicine.com/medline/citation/11524948/[Study_of_the_binding_of_the_S7_protein_with_16S_rRNA_fragment_926_986/1219_1393_as_a_key_step_in_the_assembly_of_the_small_subunit_of_prokaryotic_ribosomes]_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=11524948.ui DB - PRIME DP - Unbound Medicine ER -