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Effects of phytoestrogens on growth and DNA integrity in human prostate tumor cell lines: PC-3 and LNCaP.
Nutr Cancer 2000; 38(2):223-8NC

Abstract

Prostate cancer is one of the most common male cancers in Western countries, yet the incidence of this fatal disease remains low in Asian populations. Environmental factors such as diet play an important role in hormone-dependent cancer etiology, and a high phytoestrogen intake may be one factor contributing to the low prostate cancer mortality in Eastern populations. In this study, we investigated the effects of the phytoestrogens genistein, daidzein, coumestrol, and equol on cell growth and DNA damage (strand breakage) in two human prostate tumor cell lines: androgen receptor-positive LNCaP and androgen receptor-negative PC-3. Each compound caused growth inhibition at physiologically relevant concentrations (<10 microM). Genistein induced DNA damage in both cell lines at <10 microM. Daidzein inhibited cell growth at 10-100 microM yet had no effect on DNA damage at up to 500 microM. Thus, despite their structural similarities, different phytoestrogens inhibit prostate tumor cell growth by independent mechanisms.

Authors+Show Affiliations

Division of Cellular Integrity, Rowett Research Institute, Bucksburn, Aberdeen, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11525601

Citation

Mitchell, J H., et al. "Effects of Phytoestrogens On Growth and DNA Integrity in Human Prostate Tumor Cell Lines: PC-3 and LNCaP." Nutrition and Cancer, vol. 38, no. 2, 2000, pp. 223-8.
Mitchell JH, Duthie SJ, Collins AR. Effects of phytoestrogens on growth and DNA integrity in human prostate tumor cell lines: PC-3 and LNCaP. Nutr Cancer. 2000;38(2):223-8.
Mitchell, J. H., Duthie, S. J., & Collins, A. R. (2000). Effects of phytoestrogens on growth and DNA integrity in human prostate tumor cell lines: PC-3 and LNCaP. Nutrition and Cancer, 38(2), pp. 223-8.
Mitchell JH, Duthie SJ, Collins AR. Effects of Phytoestrogens On Growth and DNA Integrity in Human Prostate Tumor Cell Lines: PC-3 and LNCaP. Nutr Cancer. 2000;38(2):223-8. PubMed PMID: 11525601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of phytoestrogens on growth and DNA integrity in human prostate tumor cell lines: PC-3 and LNCaP. AU - Mitchell,J H, AU - Duthie,S J, AU - Collins,A R, PY - 2001/8/30/pubmed PY - 2002/1/5/medline PY - 2001/8/30/entrez SP - 223 EP - 8 JF - Nutrition and cancer JO - Nutr Cancer VL - 38 IS - 2 N2 - Prostate cancer is one of the most common male cancers in Western countries, yet the incidence of this fatal disease remains low in Asian populations. Environmental factors such as diet play an important role in hormone-dependent cancer etiology, and a high phytoestrogen intake may be one factor contributing to the low prostate cancer mortality in Eastern populations. In this study, we investigated the effects of the phytoestrogens genistein, daidzein, coumestrol, and equol on cell growth and DNA damage (strand breakage) in two human prostate tumor cell lines: androgen receptor-positive LNCaP and androgen receptor-negative PC-3. Each compound caused growth inhibition at physiologically relevant concentrations (<10 microM). Genistein induced DNA damage in both cell lines at <10 microM. Daidzein inhibited cell growth at 10-100 microM yet had no effect on DNA damage at up to 500 microM. Thus, despite their structural similarities, different phytoestrogens inhibit prostate tumor cell growth by independent mechanisms. SN - 0163-5581 UR - https://www.unboundmedicine.com/medline/citation/11525601/Effects_of_phytoestrogens_on_growth_and_DNA_integrity_in_human_prostate_tumor_cell_lines:_PC_3_and_LNCaP_ L2 - http://www.tandfonline.com/doi/full/10.1207/S15327914NC382_12 DB - PRIME DP - Unbound Medicine ER -