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Enzyme-linked immunosorbent assay for detecting herpesvirus exposure in Mediterranean tortoises (spur-thighed tortoise [Testudo graeca] and Hermann's tortoise [Testudo hermanni]).
J Clin Microbiol 2001; 39(9):3156-63JC

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies to a herpesvirus associated with an upper respiratory tract disease in Mediterranean tortoises [spur-thighed tortoise (Testudo graeca) and Hermann's tortoise (Testudo hermanni)]. This serodiagnostic test was validated through a hyperimmunization study. The mean of the A(405) readings of the plasma samples collected at time zero of the hyperimmunization study plus three times the standard deviation was used as the cutoff for seropositivity in tortoises. ELISA results were compared to serum neutralization (SN) values for the same samples by using the McNemar test. The results obtained by SN and ELISA were not significantly different (P > 0.05). This new ELISA could be used as an important diagnostic tool for screening wild populations and private and zoo collections of Mediterranean tortoises.

Authors+Show Affiliations

Department of Small Animal Clinical Sciences, University of Florida, Gainesville, Florida 32610-0126, USA. origgif@mail.vetmed.ufl.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Studies
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

11526144

Citation

Origgi, F C., et al. "Enzyme-linked Immunosorbent Assay for Detecting Herpesvirus Exposure in Mediterranean Tortoises (spur-thighed Tortoise [Testudo Graeca] and Hermann's Tortoise [Testudo Hermanni])." Journal of Clinical Microbiology, vol. 39, no. 9, 2001, pp. 3156-63.
Origgi FC, Klein PA, Mathes K, et al. Enzyme-linked immunosorbent assay for detecting herpesvirus exposure in Mediterranean tortoises (spur-thighed tortoise [Testudo graeca] and Hermann's tortoise [Testudo hermanni]). J Clin Microbiol. 2001;39(9):3156-63.
Origgi, F. C., Klein, P. A., Mathes, K., Blahak, S., Marschang, R. E., Tucker, S. J., & Jacobson, E. R. (2001). Enzyme-linked immunosorbent assay for detecting herpesvirus exposure in Mediterranean tortoises (spur-thighed tortoise [Testudo graeca] and Hermann's tortoise [Testudo hermanni]). Journal of Clinical Microbiology, 39(9), pp. 3156-63.
Origgi FC, et al. Enzyme-linked Immunosorbent Assay for Detecting Herpesvirus Exposure in Mediterranean Tortoises (spur-thighed Tortoise [Testudo Graeca] and Hermann's Tortoise [Testudo Hermanni]). J Clin Microbiol. 2001;39(9):3156-63. PubMed PMID: 11526144.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enzyme-linked immunosorbent assay for detecting herpesvirus exposure in Mediterranean tortoises (spur-thighed tortoise [Testudo graeca] and Hermann's tortoise [Testudo hermanni]). AU - Origgi,F C, AU - Klein,P A, AU - Mathes,K, AU - Blahak,S, AU - Marschang,R E, AU - Tucker,S J, AU - Jacobson,E R, PY - 2001/8/30/pubmed PY - 2002/1/5/medline PY - 2001/8/30/entrez SP - 3156 EP - 63 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 39 IS - 9 N2 - An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies to a herpesvirus associated with an upper respiratory tract disease in Mediterranean tortoises [spur-thighed tortoise (Testudo graeca) and Hermann's tortoise (Testudo hermanni)]. This serodiagnostic test was validated through a hyperimmunization study. The mean of the A(405) readings of the plasma samples collected at time zero of the hyperimmunization study plus three times the standard deviation was used as the cutoff for seropositivity in tortoises. ELISA results were compared to serum neutralization (SN) values for the same samples by using the McNemar test. The results obtained by SN and ELISA were not significantly different (P > 0.05). This new ELISA could be used as an important diagnostic tool for screening wild populations and private and zoo collections of Mediterranean tortoises. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/11526144/Enzyme_linked_immunosorbent_assay_for_detecting_herpesvirus_exposure_in_Mediterranean_tortoises__spur_thighed_tortoise_[Testudo_graeca]_and_Hermann's_tortoise_[Testudo_hermanni]__ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=11526144 DB - PRIME DP - Unbound Medicine ER -