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Determination of vigabatrin in human plasma and urine by high-performance liquid chromatography with fluorescence detection.
J Chromatogr B Biomed Sci Appl. 2001 Sep 05; 760(2):207-12.JC

Abstract

A sensitive and specific HPLC method has been developed for the assay of vigabatrin in human plasma and urine. The assay involves derivatization with 4-chloro-7-nitrobenzofurazan, solid-phase extraction on a silica column and isocratic reversed-phase chromatography with fluorescence detection. Aspartam was used as an internal standard. The assay was linear over the concentration range of 0.2-20.0 microg/ml for plasma and 1.0-15.0 microg/ml for urine with a lower limit of detection of 0.1 microg/ml using 0.1 ml of starting volume of the sample. Both the within-day and day-to-day reproducibilities and accuracies were less than 5.46% and 1.6%, respectively. After a single oral dose of 500 mg of vigabatrin, the plasma concentration and the cumulative urinary excretion of the drug were determined.

Authors+Show Affiliations

Erturk S
University of Istanbul, Faculty of Pharmacy, Department of Analytical Chemistry, Beyazit, Turkey.
Aktas ES
No affiliation info available
Atmaca S
No affiliation info available

MeSH

AdultAnticonvulsantsChromatography, High Pressure LiquidFemaleHumansMiddle AgedReproducibility of ResultsSensitivity and SpecificitySpectrometry, FluorescenceVigabatrin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11530978

Citation

Erturk, S, et al. "Determination of Vigabatrin in Human Plasma and Urine By High-performance Liquid Chromatography With Fluorescence Detection." Journal of Chromatography. B, Biomedical Sciences and Applications, vol. 760, no. 2, 2001, pp. 207-12.
Erturk S, Aktas ES, Atmaca S. Determination of vigabatrin in human plasma and urine by high-performance liquid chromatography with fluorescence detection. J Chromatogr B Biomed Sci Appl. 2001;760(2):207-12.
Erturk, S., Aktas, E. S., & Atmaca, S. (2001). Determination of vigabatrin in human plasma and urine by high-performance liquid chromatography with fluorescence detection. Journal of Chromatography. B, Biomedical Sciences and Applications, 760(2), 207-12.
Erturk S, Aktas ES, Atmaca S. Determination of Vigabatrin in Human Plasma and Urine By High-performance Liquid Chromatography With Fluorescence Detection. J Chromatogr B Biomed Sci Appl. 2001 Sep 5;760(2):207-12. PubMed PMID: 11530978.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of vigabatrin in human plasma and urine by high-performance liquid chromatography with fluorescence detection. AU - Erturk,S, AU - Aktas,E S, AU - Atmaca,S, PY - 2001/9/4/pubmed PY - 2002/1/17/medline PY - 2001/9/4/entrez SP - 207 EP - 12 JF - Journal of chromatography. B, Biomedical sciences and applications JO - J Chromatogr B Biomed Sci Appl VL - 760 IS - 2 N2 - A sensitive and specific HPLC method has been developed for the assay of vigabatrin in human plasma and urine. The assay involves derivatization with 4-chloro-7-nitrobenzofurazan, solid-phase extraction on a silica column and isocratic reversed-phase chromatography with fluorescence detection. Aspartam was used as an internal standard. The assay was linear over the concentration range of 0.2-20.0 microg/ml for plasma and 1.0-15.0 microg/ml for urine with a lower limit of detection of 0.1 microg/ml using 0.1 ml of starting volume of the sample. Both the within-day and day-to-day reproducibilities and accuracies were less than 5.46% and 1.6%, respectively. After a single oral dose of 500 mg of vigabatrin, the plasma concentration and the cumulative urinary excretion of the drug were determined. SN - 1387-2273 UR - https://www.unboundmedicine.com/medline/citation/11530978/Determination_of_vigabatrin_in_human_plasma_and_urine_by_high_performance_liquid_chromatography_with_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -