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Dietary (n-3) polyunsaturated fatty acids exert antihypertensive effects by modulating calcium signaling in T cells of rats.
J Nutr. 2001 Sep; 131(9):2364-9.JN

Abstract

After 10 wk of feeding an experimental diet enriched with (n-3) polyunsaturated fatty acids (PUFA), i.e., eicosapentaenoic acid [EPA, 20:5(n-3)] and [DHA, 22:6(n-3)] (EPAX), blood pressure in spontaneously hypertensive rats (SHR), but not in normotensive Wistar-Kyoto (WKY) rats was reduced relative to rats fed an unsupplemented control diet. Concanavalin A-stimulated T-cell proliferation was diminished in both strains of rats fed the PUFA/EPAX diet. The experimental diet lowered secretion of interleukin-2 in SHR, but not in WKY rats compared with rats fed the control diet. To determine whether there was a defect in calcium homeostasis in T cells during hypertension, we employed the following agents: caffeine, which recruits calcium from the cytosolic Ca(2+)-induced Ca(2+)-release pool; ionomycin, which at low concentrations opens calcium channels; and thapsigargin (TG), which mobilizes [Ca(2+)]i from the endoplasmic reticulum (ER) pool. Caffeine-induced increases in [Ca(2+)]i were not modified by the PUFA/EPAX diet. The ionomycin-induced increases in [Ca(2+)]i in T cells from SHR were greater than in those from WKY rats; consumption of the PUFA/EPAX diet did not modify Ca(2+) influx in cells of either strain. The TG-induced increases in [Ca(2+)]i in T cells from SHR were greater than those in cells from WKY rats. Interestingly, consumption of the experimental diet reduced TG-evoked increases in [Ca(2+)]i in T cells from SHR and increased those in T cells from WKY rats, indicating that the PUFA/EPAX diet could reverse the calcium mobilization from the ER pool in T cells. These results suggest that (n-3) PUFA exert antihypertensive effects and modulate T-cell calcium signaling during hypertension in rats.

Authors+Show Affiliations

UPRES Lipids & Nutrition, Faculty of Life Sciences, Université de Bourgogne, Dijon 21000, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11533280

Citation

Triboulot, C, et al. "Dietary (n-3) Polyunsaturated Fatty Acids Exert Antihypertensive Effects By Modulating Calcium Signaling in T Cells of Rats." The Journal of Nutrition, vol. 131, no. 9, 2001, pp. 2364-9.
Triboulot C, Hichami A, Denys A, et al. Dietary (n-3) polyunsaturated fatty acids exert antihypertensive effects by modulating calcium signaling in T cells of rats. J Nutr. 2001;131(9):2364-9.
Triboulot, C., Hichami, A., Denys, A., & Khan, N. A. (2001). Dietary (n-3) polyunsaturated fatty acids exert antihypertensive effects by modulating calcium signaling in T cells of rats. The Journal of Nutrition, 131(9), 2364-9.
Triboulot C, et al. Dietary (n-3) Polyunsaturated Fatty Acids Exert Antihypertensive Effects By Modulating Calcium Signaling in T Cells of Rats. J Nutr. 2001;131(9):2364-9. PubMed PMID: 11533280.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary (n-3) polyunsaturated fatty acids exert antihypertensive effects by modulating calcium signaling in T cells of rats. AU - Triboulot,C, AU - Hichami,A, AU - Denys,A, AU - Khan,N A, PY - 2001/9/5/pubmed PY - 2001/10/19/medline PY - 2001/9/5/entrez SP - 2364 EP - 9 JF - The Journal of nutrition JO - J Nutr VL - 131 IS - 9 N2 - After 10 wk of feeding an experimental diet enriched with (n-3) polyunsaturated fatty acids (PUFA), i.e., eicosapentaenoic acid [EPA, 20:5(n-3)] and [DHA, 22:6(n-3)] (EPAX), blood pressure in spontaneously hypertensive rats (SHR), but not in normotensive Wistar-Kyoto (WKY) rats was reduced relative to rats fed an unsupplemented control diet. Concanavalin A-stimulated T-cell proliferation was diminished in both strains of rats fed the PUFA/EPAX diet. The experimental diet lowered secretion of interleukin-2 in SHR, but not in WKY rats compared with rats fed the control diet. To determine whether there was a defect in calcium homeostasis in T cells during hypertension, we employed the following agents: caffeine, which recruits calcium from the cytosolic Ca(2+)-induced Ca(2+)-release pool; ionomycin, which at low concentrations opens calcium channels; and thapsigargin (TG), which mobilizes [Ca(2+)]i from the endoplasmic reticulum (ER) pool. Caffeine-induced increases in [Ca(2+)]i were not modified by the PUFA/EPAX diet. The ionomycin-induced increases in [Ca(2+)]i in T cells from SHR were greater than in those from WKY rats; consumption of the PUFA/EPAX diet did not modify Ca(2+) influx in cells of either strain. The TG-induced increases in [Ca(2+)]i in T cells from SHR were greater than those in cells from WKY rats. Interestingly, consumption of the experimental diet reduced TG-evoked increases in [Ca(2+)]i in T cells from SHR and increased those in T cells from WKY rats, indicating that the PUFA/EPAX diet could reverse the calcium mobilization from the ER pool in T cells. These results suggest that (n-3) PUFA exert antihypertensive effects and modulate T-cell calcium signaling during hypertension in rats. SN - 0022-3166 UR - https://www.unboundmedicine.com/medline/citation/11533280/Dietary__n_3__polyunsaturated_fatty_acids_exert_antihypertensive_effects_by_modulating_calcium_signaling_in_T_cells_of_rats_ L2 - https://academic.oup.com/jn/article-lookup/doi/10.1093/jn/131.9.2364 DB - PRIME DP - Unbound Medicine ER -