Tags

Type your tag names separated by a space and hit enter

CD4/CD8 lymphocytes in BALF during the efferent phase of lung delayed-type hypersensitivity reaction induced by single antigen inhalation.
Med Sci Monit. 2001 Sep-Oct; 7(5):878-83.MS

Abstract

BACKGROUND

The precise mechanisms involved in the pathogenesis of hypersensitivity pneumonitis (HP) have not been identified. HP is characterized by inflammatory lymphocytic alveolitis and a remarkable increase in T-lymphocytes detected in bronchoalveolar lavage fluid (BALF). It is suggested that both CD4+ and CD8+ T cells may contribute to the pathogenesis of HP. Experiments on animal models suggest that cell mediated immunity (CMI) is more important for the pathogenesis of HP than complex-mediated immunity, but the relationship between the subsets of BALF lymphocytes and humoral or cell-mediated allergic reactions is still not clear. The aim of our study was distinguish CD4+ and CD8+ T cells in BALF lymphocytes during a delayed-type hypersensitivity (DTH) reaction in the lung.

MATERIAL AND METHODS

The experiment was performed on guinea pigs sensitized with BCG vaccine and subjected to a single inhalation of tubercle bacilli antigens (tuberculin). 24 hours after tuberculin provocation (at the time of maximum lymphocyte infiltration), bronchoalveolar lavage was performed on both sensitized and non-sensitized (control) animals. The total cell count was estimated, and a differential microscopical examination of BAL-fluid cells was performed, along with the phenotyping of BALF lymphocytes (by flow cytometry).

RESULTS

In the BALF of the sensitized animals, as compared to the controls, there was a statistically significant increase in the percentage and absolute count of T-lymphocytes, CD4+ and CD8+. The CD4 / CD8 ratio in both groups did not differ significantly and was individually variable (2.94I0.72 SEM in the experimental group, vs 4.41I1.29 SEM in the control group).

CONCLUSIONS

Both CD4+ and CD8+ lymphocytes (with some predominance of helper cells) participate in the efferent phase of the delayed type hypersensitivity reaction in the lung induced by antigen inhalation.

Authors+Show Affiliations

Department and Clinic of Internal Medicine, Pulmonology and Allergology, Medical University, Warsaw, Poland. hgj@amwaw.edu.plNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11535927

Citation

Grubek-Jaworska, H, et al. "CD4/CD8 Lymphocytes in BALF During the Efferent Phase of Lung Delayed-type Hypersensitivity Reaction Induced By Single Antigen Inhalation." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 7, no. 5, 2001, pp. 878-83.
Grubek-Jaworska H, Hoser G, Droszcz P, et al. CD4/CD8 lymphocytes in BALF during the efferent phase of lung delayed-type hypersensitivity reaction induced by single antigen inhalation. Med Sci Monit. 2001;7(5):878-83.
Grubek-Jaworska, H., Hoser, G., Droszcz, P., & Chazan, R. (2001). CD4/CD8 lymphocytes in BALF during the efferent phase of lung delayed-type hypersensitivity reaction induced by single antigen inhalation. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 7(5), 878-83.
Grubek-Jaworska H, et al. CD4/CD8 Lymphocytes in BALF During the Efferent Phase of Lung Delayed-type Hypersensitivity Reaction Induced By Single Antigen Inhalation. Med Sci Monit. 2001 Sep-Oct;7(5):878-83. PubMed PMID: 11535927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CD4/CD8 lymphocytes in BALF during the efferent phase of lung delayed-type hypersensitivity reaction induced by single antigen inhalation. AU - Grubek-Jaworska,H, AU - Hoser,G, AU - Droszcz,P, AU - Chazan,R, PY - 2001/9/6/pubmed PY - 2002/1/18/medline PY - 2001/9/6/entrez SP - 878 EP - 83 JF - Medical science monitor : international medical journal of experimental and clinical research JO - Med Sci Monit VL - 7 IS - 5 N2 - BACKGROUND: The precise mechanisms involved in the pathogenesis of hypersensitivity pneumonitis (HP) have not been identified. HP is characterized by inflammatory lymphocytic alveolitis and a remarkable increase in T-lymphocytes detected in bronchoalveolar lavage fluid (BALF). It is suggested that both CD4+ and CD8+ T cells may contribute to the pathogenesis of HP. Experiments on animal models suggest that cell mediated immunity (CMI) is more important for the pathogenesis of HP than complex-mediated immunity, but the relationship between the subsets of BALF lymphocytes and humoral or cell-mediated allergic reactions is still not clear. The aim of our study was distinguish CD4+ and CD8+ T cells in BALF lymphocytes during a delayed-type hypersensitivity (DTH) reaction in the lung. MATERIAL AND METHODS: The experiment was performed on guinea pigs sensitized with BCG vaccine and subjected to a single inhalation of tubercle bacilli antigens (tuberculin). 24 hours after tuberculin provocation (at the time of maximum lymphocyte infiltration), bronchoalveolar lavage was performed on both sensitized and non-sensitized (control) animals. The total cell count was estimated, and a differential microscopical examination of BAL-fluid cells was performed, along with the phenotyping of BALF lymphocytes (by flow cytometry). RESULTS: In the BALF of the sensitized animals, as compared to the controls, there was a statistically significant increase in the percentage and absolute count of T-lymphocytes, CD4+ and CD8+. The CD4 / CD8 ratio in both groups did not differ significantly and was individually variable (2.94I0.72 SEM in the experimental group, vs 4.41I1.29 SEM in the control group). CONCLUSIONS: Both CD4+ and CD8+ lymphocytes (with some predominance of helper cells) participate in the efferent phase of the delayed type hypersensitivity reaction in the lung induced by antigen inhalation. SN - 1234-1010 UR - https://www.unboundmedicine.com/medline/citation/11535927/CD4/CD8_lymphocytes_in_BALF_during_the_efferent_phase_of_lung_delayed_type_hypersensitivity_reaction_induced_by_single_antigen_inhalation_ L2 - https://www.medscimonit.com/download/index/idArt/508553 DB - PRIME DP - Unbound Medicine ER -