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Effects of hypothermic machine perfusion on rat liver function depending on the route of perfusion.
Transplantation 2001; 72(4):606-14T

Abstract

BACKGROUND AND METHODS

The aim of this study was to evaluate the efficacy of hypothermic machine perfusion (HMP) to preserve rat livers according to the route of perfusion, i.e., via portal vein, hepatic veins (retrograde), or hepatic artery. Livers were preserved for 24 or 48 hr by simple cold storage (SCS) or by HMP. Preservation solution was supplemented with (HMP) or without (SCS) hydroxyethyl starch. After preservation, grafts were reperfused for 2 hr with an oxygenated Krebs-Henseleit bicarbonate buffer.

RESULTS

After 24 hr of preservation, total glutathione concentrations in HMP livers were similar (1287+/-37, 1418+/-118, and 1471+/-62 nmol/g in hepatic artery, portal vein, and hepatic vein HMP livers, respectively) and higher than in the SCS (833+/-118 nmol/g, P<0.05) group. These higher total glutathione values were due to higher reduced glutathione concentrations. ATP concentrations in the liver tissue were similar in HMP groups (0.75+/-0.4, 0.64+/-0.1, and 0.77+/-0.1 micromol/g in hepatic artery, portal vein, and hepatic vein HMP livers, respectively) and higher than in SCS (0.32+/-0.06 micromol/g, P<0.05). After 2 hr of normothermic reperfusion, bile production in the HMP portal and HMP retrograde groups were similar (391+/-29 ml and 372+/-25 ml) and higher than in the HMP artery or SCS groups (275+/-25 ml and 277+/-32 ml, respectively; P<0.05). Aspartate transaminase, alanine transaminase, lactate dehydrogenase, and purine nucleoside phosphorylase release into the perfusate of HMP portal and HMP retrograde perfused livers was similar and significantly lower compared to the HMP artery and SCS groups. At the end of reperfusion, no statistical differences were found for glutathione concentration and energetic reserves in the livers of each group. After 48 hr of preservation, livers from the HMP portal and HMP retrograde groups did significantly better than livers from the HMP artery or SCS groups.

CONCLUSIONS

This study confirms the superiority of HMP over SCS to preserve the liver graft. It shows that retrograde perfusion is similar to PV perfusion and that perfusion by HA is less beneficial.

Authors+Show Affiliations

INSERM U-456, Detoxification and Tissue Repair Unit, University of Rennes I, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11544418

Citation

Compagnon, P, et al. "Effects of Hypothermic Machine Perfusion On Rat Liver Function Depending On the Route of Perfusion." Transplantation, vol. 72, no. 4, 2001, pp. 606-14.
Compagnon P, Clément B, Campion JP, et al. Effects of hypothermic machine perfusion on rat liver function depending on the route of perfusion. Transplantation. 2001;72(4):606-14.
Compagnon, P., Clément, B., Campion, J. P., & Boudjema, K. (2001). Effects of hypothermic machine perfusion on rat liver function depending on the route of perfusion. Transplantation, 72(4), pp. 606-14.
Compagnon P, et al. Effects of Hypothermic Machine Perfusion On Rat Liver Function Depending On the Route of Perfusion. Transplantation. 2001 Aug 27;72(4):606-14. PubMed PMID: 11544418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of hypothermic machine perfusion on rat liver function depending on the route of perfusion. AU - Compagnon,P, AU - Clément,B, AU - Campion,J P, AU - Boudjema,K, PY - 2001/9/7/pubmed PY - 2001/9/21/medline PY - 2001/9/7/entrez SP - 606 EP - 14 JF - Transplantation JO - Transplantation VL - 72 IS - 4 N2 - BACKGROUND AND METHODS: The aim of this study was to evaluate the efficacy of hypothermic machine perfusion (HMP) to preserve rat livers according to the route of perfusion, i.e., via portal vein, hepatic veins (retrograde), or hepatic artery. Livers were preserved for 24 or 48 hr by simple cold storage (SCS) or by HMP. Preservation solution was supplemented with (HMP) or without (SCS) hydroxyethyl starch. After preservation, grafts were reperfused for 2 hr with an oxygenated Krebs-Henseleit bicarbonate buffer. RESULTS: After 24 hr of preservation, total glutathione concentrations in HMP livers were similar (1287+/-37, 1418+/-118, and 1471+/-62 nmol/g in hepatic artery, portal vein, and hepatic vein HMP livers, respectively) and higher than in the SCS (833+/-118 nmol/g, P<0.05) group. These higher total glutathione values were due to higher reduced glutathione concentrations. ATP concentrations in the liver tissue were similar in HMP groups (0.75+/-0.4, 0.64+/-0.1, and 0.77+/-0.1 micromol/g in hepatic artery, portal vein, and hepatic vein HMP livers, respectively) and higher than in SCS (0.32+/-0.06 micromol/g, P<0.05). After 2 hr of normothermic reperfusion, bile production in the HMP portal and HMP retrograde groups were similar (391+/-29 ml and 372+/-25 ml) and higher than in the HMP artery or SCS groups (275+/-25 ml and 277+/-32 ml, respectively; P<0.05). Aspartate transaminase, alanine transaminase, lactate dehydrogenase, and purine nucleoside phosphorylase release into the perfusate of HMP portal and HMP retrograde perfused livers was similar and significantly lower compared to the HMP artery and SCS groups. At the end of reperfusion, no statistical differences were found for glutathione concentration and energetic reserves in the livers of each group. After 48 hr of preservation, livers from the HMP portal and HMP retrograde groups did significantly better than livers from the HMP artery or SCS groups. CONCLUSIONS: This study confirms the superiority of HMP over SCS to preserve the liver graft. It shows that retrograde perfusion is similar to PV perfusion and that perfusion by HA is less beneficial. SN - 0041-1337 UR - https://www.unboundmedicine.com/medline/citation/11544418/Effects_of_hypothermic_machine_perfusion_on_rat_liver_function_depending_on_the_route_of_perfusion_ L2 - http://dx.doi.org/10.1097/00007890-200108270-00008 DB - PRIME DP - Unbound Medicine ER -