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Electrophoretic separation of tryptophan enantiomers in biological samples.
Electrophoresis. 2001 Aug; 22(13):2769-74.E

Abstract

A method for the determination of D- and L-tryptophan (Trp) in biological samples is described. The amino acid enantiomers were precolumn-derivatized with a fluorescence tagging reagent, naphthalene-2,3-dialdehyde (NDA). In the presence of hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD) as the chiral selector, NDA-tagged Trp enantiomers were well resolved by micellar electrokinetic chromatography (MEKC). Using laser induced fluorescence (LIF) detection, a detection limit of 3.3 x 10(-8) M Trp was obtained. The method was applied to the determination of Trp enantiomers in biological samples including human urine and cerebrospinal fluid (CSF), rat brain tissue, and Aplysia ganglia. No interference from other amino acids or the endogenous compounds in the sample matrices was observed. D-Trp was found at the sub-microM level in human urine samples collected from several healthy subjects. Further, the determination of DL-Trp residues in small quantities (10 microg) of peptides after acid hydrolysis is demonstrated.

Authors+Show Affiliations

Zhao S
Department of Chemistry, Jackson State University, MS, USA.
Liu YM
No affiliation info available

MeSH

Chromatography, Micellar Electrokinetic CapillaryHumansIndicators and ReagentsNaphthalenesTryptophan

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11545406

Citation

Zhao, S, and Y M. Liu. "Electrophoretic Separation of Tryptophan Enantiomers in Biological Samples." Electrophoresis, vol. 22, no. 13, 2001, pp. 2769-74.
Zhao S, Liu YM. Electrophoretic separation of tryptophan enantiomers in biological samples. Electrophoresis. 2001;22(13):2769-74.
Zhao, S., & Liu, Y. M. (2001). Electrophoretic separation of tryptophan enantiomers in biological samples. Electrophoresis, 22(13), 2769-74.
Zhao S, Liu YM. Electrophoretic Separation of Tryptophan Enantiomers in Biological Samples. Electrophoresis. 2001;22(13):2769-74. PubMed PMID: 11545406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrophoretic separation of tryptophan enantiomers in biological samples. AU - Zhao,S, AU - Liu,Y M, PY - 2001/9/8/pubmed PY - 2002/1/29/medline PY - 2001/9/8/entrez SP - 2769 EP - 74 JF - Electrophoresis JO - Electrophoresis VL - 22 IS - 13 N2 - A method for the determination of D- and L-tryptophan (Trp) in biological samples is described. The amino acid enantiomers were precolumn-derivatized with a fluorescence tagging reagent, naphthalene-2,3-dialdehyde (NDA). In the presence of hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD) as the chiral selector, NDA-tagged Trp enantiomers were well resolved by micellar electrokinetic chromatography (MEKC). Using laser induced fluorescence (LIF) detection, a detection limit of 3.3 x 10(-8) M Trp was obtained. The method was applied to the determination of Trp enantiomers in biological samples including human urine and cerebrospinal fluid (CSF), rat brain tissue, and Aplysia ganglia. No interference from other amino acids or the endogenous compounds in the sample matrices was observed. D-Trp was found at the sub-microM level in human urine samples collected from several healthy subjects. Further, the determination of DL-Trp residues in small quantities (10 microg) of peptides after acid hydrolysis is demonstrated. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/11545406/Electrophoretic_separation_of_tryptophan_enantiomers_in_biological_samples_ L2 - https://doi.org/10.1002/1522-2683(200108)22:13<2769::AID-ELPS2769>3.0.CO;2-H DB - PRIME DP - Unbound Medicine ER -