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Direct electrochemical determination of pyruvate in human sweat by capillary zone electrophoresis.
Electrophoresis. 2001 Aug; 22(13):2793-6.E

Abstract

Capillary zone electrophoresis was employed for the determination of pyruvate in human sweat using electrochemical detection with a carbon fiber microdisk bundle electrode at a constant potential of 1.60 V vs. saturated calomel electrode. The optimum separation conditions are 3.6 x 10(-3) mol/L Na2HPO4-1.4 x 10(-3) mol/L NaH2PO4 (pH 7.2) for the buffer solution, and 18 kV for the separation voltage. The limits of detection of pyruvate are 8.0 x 10(-6) mol/L or 24 fmol (S/N = 3) for the injection voltage of 6 kV and the injection time of 10 s. The relative standard deviation is 2.0% for the migration time and 5.7% for the electrophoretic peak current. The method was applied to determining pyruvate in human sweat.

Authors+Show Affiliations

Jin M
Laboratory of Analytical Science, School of Chemistry, Shandong University, Jinan, PR China.
Dong Q
No affiliation info available
Dong R
No affiliation info available
Jin W
No affiliation info available

MeSH

ElectrochemistryElectrophoresis, CapillaryHumansLinear ModelsPyruvic AcidReproducibility of ResultsSweat

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11545410

Citation

Jin, M, et al. "Direct Electrochemical Determination of Pyruvate in Human Sweat By Capillary Zone Electrophoresis." Electrophoresis, vol. 22, no. 13, 2001, pp. 2793-6.
Jin M, Dong Q, Dong R, et al. Direct electrochemical determination of pyruvate in human sweat by capillary zone electrophoresis. Electrophoresis. 2001;22(13):2793-6.
Jin, M., Dong, Q., Dong, R., & Jin, W. (2001). Direct electrochemical determination of pyruvate in human sweat by capillary zone electrophoresis. Electrophoresis, 22(13), 2793-6.
Jin M, et al. Direct Electrochemical Determination of Pyruvate in Human Sweat By Capillary Zone Electrophoresis. Electrophoresis. 2001;22(13):2793-6. PubMed PMID: 11545410.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct electrochemical determination of pyruvate in human sweat by capillary zone electrophoresis. AU - Jin,M, AU - Dong,Q, AU - Dong,R, AU - Jin,W, PY - 2001/9/8/pubmed PY - 2002/1/29/medline PY - 2001/9/8/entrez SP - 2793 EP - 6 JF - Electrophoresis JO - Electrophoresis VL - 22 IS - 13 N2 - Capillary zone electrophoresis was employed for the determination of pyruvate in human sweat using electrochemical detection with a carbon fiber microdisk bundle electrode at a constant potential of 1.60 V vs. saturated calomel electrode. The optimum separation conditions are 3.6 x 10(-3) mol/L Na2HPO4-1.4 x 10(-3) mol/L NaH2PO4 (pH 7.2) for the buffer solution, and 18 kV for the separation voltage. The limits of detection of pyruvate are 8.0 x 10(-6) mol/L or 24 fmol (S/N = 3) for the injection voltage of 6 kV and the injection time of 10 s. The relative standard deviation is 2.0% for the migration time and 5.7% for the electrophoretic peak current. The method was applied to determining pyruvate in human sweat. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/11545410/Direct_electrochemical_determination_of_pyruvate_in_human_sweat_by_capillary_zone_electrophoresis_ L2 - https://doi.org/10.1002/1522-2683(200108)22:13<2793::AID-ELPS2793>3.0.CO;2-A DB - PRIME DP - Unbound Medicine ER -