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Functional comparison of the single-layer agarose microbeads and the developed three-layer agarose microbeads as the bioartificial pancreas: an in vitro study.
Cell Transplant. 2001; 10(4-5):403-8.CT

Abstract

In this study, the insulin secretory characteristics of the microencapsulated hamster islets were studied during long-term culture. The hamster islets were encapsulated as single-layer agarose microbeads or three-layer agarose microbeads with agarose and agarose containing poly(styrene sulfonic acid) (PSSa), respectively. The influence of PSSa on the function of the rat islets microencapsulted in three-layer microbeads was primarily monitored. The aim of this study was to examine the influence of the PSSa on the in vitro function of the islets encapsulated in the agarose/PSSa microbeads compared with single-layer agarose microbeads during long-term culture. The microbeads were cultured for 30 days in medium of Eagle's MEM at 37 degrees C in 5% CO2 and 95% air. The basal insulin secretion into the culture medium was measured daily during the first 12 days and two times per week until 30 days. The microbeads were subjected to static incubation test on the 10th, 20th, and 30th day during culture. The basal insulin secretion level of the agarose/PSSa microbeads was significantly higher than that of single-layer agarose microbeads. The static incubation tests revealed a similar pattern of insulin secretion from both microbeads when they were exposed to high glucose challenge. In the static incubation test, both could significantly increase insulin release to more than 6.61 times (stimulation index) in response to high glucose stimulation and could significantly decrease when glucose concentration returned from high glucose to low glucose on the 10th, 20th, and 30th day of culture. This study demonstrated that the hamster islets enclosed in agarose/PSSa hydrogel not only continuously secreted basal amounts of insulin, but also maintained their response to high glucose stimulation similar to the agarose microbeads. The above results together with those of our previous in vivo study suggest that the three-layer microbeads (agarose/PSSa) are well suitable for xenotransplantation of islets for the clinical application.

Authors+Show Affiliations

First Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Japan. xubaoyou@frontier.kyoto-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11549062

Citation

Xu, B, et al. "Functional Comparison of the Single-layer Agarose Microbeads and the Developed Three-layer Agarose Microbeads as the Bioartificial Pancreas: an in Vitro Study." Cell Transplantation, vol. 10, no. 4-5, 2001, pp. 403-8.
Xu B, Iwata H, Miyamoto M, et al. Functional comparison of the single-layer agarose microbeads and the developed three-layer agarose microbeads as the bioartificial pancreas: an in vitro study. Cell Transplant. 2001;10(4-5):403-8.
Xu, B., Iwata, H., Miyamoto, M., Balamurugan, A. N., Murakami, Y., Cui, W., Imamura, M., & Inoue, K. (2001). Functional comparison of the single-layer agarose microbeads and the developed three-layer agarose microbeads as the bioartificial pancreas: an in vitro study. Cell Transplantation, 10(4-5), 403-8.
Xu B, et al. Functional Comparison of the Single-layer Agarose Microbeads and the Developed Three-layer Agarose Microbeads as the Bioartificial Pancreas: an in Vitro Study. Cell Transplant. 2001;10(4-5):403-8. PubMed PMID: 11549062.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional comparison of the single-layer agarose microbeads and the developed three-layer agarose microbeads as the bioartificial pancreas: an in vitro study. AU - Xu,B, AU - Iwata,H, AU - Miyamoto,M, AU - Balamurugan,A N, AU - Murakami,Y, AU - Cui,W, AU - Imamura,M, AU - Inoue,K, PY - 2001/9/11/pubmed PY - 2002/4/18/medline PY - 2001/9/11/entrez SP - 403 EP - 8 JF - Cell transplantation JO - Cell Transplant VL - 10 IS - 4-5 N2 - In this study, the insulin secretory characteristics of the microencapsulated hamster islets were studied during long-term culture. The hamster islets were encapsulated as single-layer agarose microbeads or three-layer agarose microbeads with agarose and agarose containing poly(styrene sulfonic acid) (PSSa), respectively. The influence of PSSa on the function of the rat islets microencapsulted in three-layer microbeads was primarily monitored. The aim of this study was to examine the influence of the PSSa on the in vitro function of the islets encapsulated in the agarose/PSSa microbeads compared with single-layer agarose microbeads during long-term culture. The microbeads were cultured for 30 days in medium of Eagle's MEM at 37 degrees C in 5% CO2 and 95% air. The basal insulin secretion into the culture medium was measured daily during the first 12 days and two times per week until 30 days. The microbeads were subjected to static incubation test on the 10th, 20th, and 30th day during culture. The basal insulin secretion level of the agarose/PSSa microbeads was significantly higher than that of single-layer agarose microbeads. The static incubation tests revealed a similar pattern of insulin secretion from both microbeads when they were exposed to high glucose challenge. In the static incubation test, both could significantly increase insulin release to more than 6.61 times (stimulation index) in response to high glucose stimulation and could significantly decrease when glucose concentration returned from high glucose to low glucose on the 10th, 20th, and 30th day of culture. This study demonstrated that the hamster islets enclosed in agarose/PSSa hydrogel not only continuously secreted basal amounts of insulin, but also maintained their response to high glucose stimulation similar to the agarose microbeads. The above results together with those of our previous in vivo study suggest that the three-layer microbeads (agarose/PSSa) are well suitable for xenotransplantation of islets for the clinical application. SN - 0963-6897 UR - https://www.unboundmedicine.com/medline/citation/11549062/Functional_comparison_of_the_single_layer_agarose_microbeads_and_the_developed_three_layer_agarose_microbeads_as_the_bioartificial_pancreas:_an_in_vitro_study_ DB - PRIME DP - Unbound Medicine ER -