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The specific isolation of complete 5S rDNA units from chromosome 1A of hexaploid, tetraploid, and diploid wheat species using PCR with head-to-head oriented primers.
Genome. 2001 Aug; 44(4):529-38.G

Abstract

The presence of 5S rDNA units on chromosome 1A of Triticum aestivum was shown by the development of a specific PCR test, using head-to-head oriented primers. This primer set allowed the amplification of complete 5S DNA units and was used to isolate SS-Rrna-A1 sequences from polyploid and diploid wheat species. Multiple-alignment and parsimony analyses of the 132 sequences divided the sequences into four types. The isolates from T. aestivum and the tetraploid species (T. dicoccoides, T. dicoccum, T durum, T. araraticum, and T timopheevi) were all of one type, which was shown to be closely related to the type mainly characteristic for T. urartu. The other two types were isolated exclusively from the diploid species T. monococcum, T aegilopoides, T. thaoudar, and T. sinskajae and the hexaploid species T. zhukovski. Triticum monococcum was the only species for which representatives of each of the four sequence types were found to be present. Further, we discuss the possible multicluster arrangement of the 5S-Rrna-A1 array.

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Authors+Show Affiliations

Van Campenhout S
Laboratory of Gene Technology, Katholieke Universiteit Leuven, Belgium. steven.vancampenhout@agr.kuleuven.ac.be
Stappen JV
No affiliation info available
Volckaert G
No affiliation info available

MeSH

Base SequenceChromosomesCloning, MolecularDNADNA PrimersDNA, RibosomalGenes, PlantGenotypeModels, GeneticMolecular Sequence DataPloidiesPolymerase Chain ReactionSequence Homology, Nucleic AcidSpecies SpecificityTriticum

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11550886

Citation

Van Campenhout, S, et al. "The Specific Isolation of Complete 5S rDNA Units From Chromosome 1A of Hexaploid, Tetraploid, and Diploid Wheat Species Using PCR With Head-to-head Oriented Primers." Genome, vol. 44, no. 4, 2001, pp. 529-38.
Van Campenhout S, Stappen JV, Volckaert G. The specific isolation of complete 5S rDNA units from chromosome 1A of hexaploid, tetraploid, and diploid wheat species using PCR with head-to-head oriented primers. Genome. 2001;44(4):529-38.
Van Campenhout, S., Stappen, J. V., & Volckaert, G. (2001). The specific isolation of complete 5S rDNA units from chromosome 1A of hexaploid, tetraploid, and diploid wheat species using PCR with head-to-head oriented primers. Genome, 44(4), 529-38.
Van Campenhout S, Stappen JV, Volckaert G. The Specific Isolation of Complete 5S rDNA Units From Chromosome 1A of Hexaploid, Tetraploid, and Diploid Wheat Species Using PCR With Head-to-head Oriented Primers. Genome. 2001;44(4):529-38. PubMed PMID: 11550886.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The specific isolation of complete 5S rDNA units from chromosome 1A of hexaploid, tetraploid, and diploid wheat species using PCR with head-to-head oriented primers. AU - Van Campenhout,S, AU - Stappen,J V, AU - Volckaert,G, PY - 2001/9/12/pubmed PY - 2002/3/15/medline PY - 2001/9/12/entrez SP - 529 EP - 38 JF - Genome JO - Genome VL - 44 IS - 4 N2 - The presence of 5S rDNA units on chromosome 1A of Triticum aestivum was shown by the development of a specific PCR test, using head-to-head oriented primers. This primer set allowed the amplification of complete 5S DNA units and was used to isolate SS-Rrna-A1 sequences from polyploid and diploid wheat species. Multiple-alignment and parsimony analyses of the 132 sequences divided the sequences into four types. The isolates from T. aestivum and the tetraploid species (T. dicoccoides, T. dicoccum, T durum, T. araraticum, and T timopheevi) were all of one type, which was shown to be closely related to the type mainly characteristic for T. urartu. The other two types were isolated exclusively from the diploid species T. monococcum, T aegilopoides, T. thaoudar, and T. sinskajae and the hexaploid species T. zhukovski. Triticum monococcum was the only species for which representatives of each of the four sequence types were found to be present. Further, we discuss the possible multicluster arrangement of the 5S-Rrna-A1 array. SN - 0831-2796 UR - https://www.unboundmedicine.com/medline/citation/11550886/The_specific_isolation_of_complete_5S_rDNA_units_from_chromosome_1A_of_hexaploid_tetraploid_and_diploid_wheat_species_using_PCR_with_head_to_head_oriented_primers_ L2 - https://www.ingentaconnect.com/openurl?genre=article&issn=0831-2796&volume=44&issue=4&spage=529&aulast=Van Campenhout DB - PRIME DP - Unbound Medicine ER -